Mammalian genomes contain active recombinase recognition sites

Gene

Volumn 244, Issue 1-2, 2000, Pages 47-54

  Thyagarajan, B ^a   Guimaraes M J ^a   Groth, A C ^a   Calos, M P ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cre; Genetic engineering; LoxP; Site specific integration

Indexed keywords

RECOMBINASE;

ARTICLE; ENZYME ANALYSIS; GENE INSERTION; GENE SEQUENCE; GENE THERAPY; GENETIC ENGINEERING; GENOME; HUMAN; HUMAN CELL; MAMMAL; MOLECULAR RECOGNITION; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

ANIMALS; BASE SEQUENCE; BINDING SITES; CELL LINE; DATABASES, FACTUAL; ESCHERICHIA COLI; GENOME; GENOME, HUMAN; HUMANS; INTEGRASES; MICE; RECOMBINANT FUSION PROTEINS; TRANSFECTION; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 0343415768     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(00)00008-1     Document Type: Article

References (24)

1. Abremski K., Hoess R. Phage P1 Cre-loxP site-specific recombination: Effects of DNA supercoiling on catenation and knotting of recombinant products. J. Biol. Chem. 184:1985;211-220.
2. Abremski K., Frommer B., Wierzbicki A., Hoess R.H. Properties of a mutant Cre protein that alters topological linkage of recombination products. J. Mol. Biol. 202:1988;59-66.
3. Albert H., Dale E., Lee E., Ow D.W. Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J. 7:1995;649-659.
4. Baubonis W., Sauer B. Genomic targeting with purified Cre recombinase. Nucleic Acids Res. 21:1993;2025-2029.
5. Benson D.A., Boguski M.S., Lipman D.J., Ostell J., Francis B.F. GenBank. Nucleic Acids Res. 26:1998;1-7.
6. Buchholz F., Angrand P.O., Stewart A.F. A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs. Nucleic Acids Res. 24:1996;3118-3119.
7. Feil R., Brocard J., Mascrez B., LeMeur M., Metzger D., Chambon P. Ligand-activated site-specific recombination in mice. Proc. Natl. Acad. Sci. USA. 93:1996;10887-10890.
8. Graham F.L., Smiley J., Russell W.C., Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36:1977;59-72.
9. Guo F., Gopaul D.N., Van Duyne G.D. Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse. Nature. 389:1997;40-46.
10. Hirt B. Selective extraction of polyoma DNA from infected mouse cultures. J. Mol. Biol. 26:1967;365-369.
11. Hoess R., Ziese M., Sternberg N. P1 site-specific recombination: nucleotide sequence of the recombining sites. Proc. Natl. Acad. Sci. USA. 79:1982;3398-3402.
12. Hoess R.H., Wierzbicki A., Abremski K. The role of the loxP spacer region in P1 site-specific recombination. Nucleic Acids Res. 14:1986;2287-2300.
13. Hoess R., Abremski K., Irwin S., Kendall M., Mack A. DNA specificity of the Cre recombinase resides in the 25 kDa carboxyl domain of the protein. J. Mol. Biol. 216:1990;873-882.
14. Kistner A., Gossen M., Zimmermann F., Jerecic J., Ullmer C., Lubbert H., Bujard H. Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc. Natl. Acad. Sci. USA. 93:1996;10933-10938.
15. Lee G., Saito I. Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination. Gene. 216:1998;55-65.
16. O'Gorman S., Fox D.T., Wahl G.M. Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science. 251:1991;1351-1355.
17. Sauer B., Henderson N. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. Proc. Natl. Acad. Sci. USA. 85:1988;5166-5170.
18. Sauer B. Multiplex Cre/lox recombination permits selective site-specific DNA targeting to both a natural and an engineered site in the yeast genome. Nucleic Acids Res. 24:1996;4608-4613.
19. Sauer B., Henderson N. Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biol. 2:1990;441-449.
20. Sauer B. Identification of cryptic lox sites in the yeast genome by seletion for Cre-mediated chromosome translocations that confer multiple drug resistance. J. Mol. Biol. 223:1992;911-928.
21. Sauer B. Site-specific recombination: developments and applications. Curr. Opin. Biotechnol. 5:1994;521-527.
22. Sternberg N., Hamilton D., Hoess R. Bacteriophage P1 site-specific recombination. II. Recombination between loxP and the bacterial chromosome. J. Mol. Biol. 150:1981;487-507.
23. Vega M.A. Prospects for homologous recombination in human gene therapy. Hum. Genet. 87:1991;245-253.
24. Verma I.M., Somia N. Gene therapy - promises, problems and prospects. Nature. 389:1997;239-242.