An efficient homologous recombination vector pTV(I) contains a hot spot for increased recombinant protein expression in Chinese hamster ovary cells

Gene

Volumn 280, Issue 1-2, 2001, Pages 87-95

  Koduri, Raju K ^a,b   Miller, John T ^a   Thammana, Pallaiah ^a  

^a BRISTOL MYERS SQUIBB COMPANY   (United States)

^b Nuvelo Inc   (United States)

Author keywords

Hot spot for increased recombinant protein expression; Inverted duplication; Matrix attachment regions; Recombinant protein expression

Indexed keywords

CYTOTOXIC T LYMPHOCYTE ANTIGEN 4; DNA; DNA FRAGMENT; PLASMID VECTOR; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CHO CELL; DNA SEQUENCE; FEMALE; GENE EXPRESSION; GENE LOCUS; GENE TARGETING; GENETIC TRANSFECTION; MAMMAL; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECOMBINANT GENE; SEQUENCE ANALYSIS; TECHNIQUE;

ANIMALS; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION; BASE SEQUENCE; BLOTTING, SOUTHERN; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; DNA; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION REGULATION; GENETIC VECTORS; HUMANS; IMMUNOCONJUGATES; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; REPETITIVE SEQUENCES, NUCLEIC ACID; SEQUENCE ANALYSIS, DNA; TRANSFECTION;

CRICETINAE; CRICETULUS GRISEUS; MAMMALIA;

EID: 0035667541     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(01)00772-7     Document Type: Article

References (33)

1. Targeted homologous recombination at the endogenous adenine phosphoribosyltransferase locus in Chinese hamster cells
2. Chromosomal insertion of foreign DNA
3. Biological significance of unwinding capability of nuclear matrix associating DNAs
4. Isolation of nucleotide sequence of an autonomously replicating sequence (ARS) elements functional in Candida albicans and Saccharomyces cerevisiae
5. Modification of DNA ends can decrease end joining relative to homologous recombination in mammalian cells
6. Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites
7. Matrix attachment regions and transcription units in a polygenic mammalian locus overlapping two isochores
8. Genomic targeting with a positive-selection in Lox integration vector allows highly reproducible gene expression in mammalian cells
9. The variability in activity of the universally expressed human cytomegalovirus immediate early gene 1 enhancer/promoter in transgenic mice
10. Position-independent, high-level expression of the human beta-globin gene in transgenic mice
11. Amplified inverted duplications within and adjacent to heterologous selectable DNA
12. Low molecular weight RNAs transcribed in vitro by RNA polymerase III from Alu-type dispersed repeats in Chinese hamster DNA are also found in vivo
13. A hot spot for novel amplification joints in a mosaic of Alu-like repeats and palindromic A + T-rich DNA
14. The multicopy appearance of a large inverted duplication and the sequence at the inversion joint suggest a new model for gene amplification
15. Co-amplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells
16. A position-effect assay for boundaries of higher order chromosomal domains
17. Genetics. Small and perfectly formed
18. A foreign beta-globin gene in transgenic mice: Integration at abnormal chromosomal positions and expression in inappropriate tissues
19. CTLA4 is a second receptor for the B cell activation antigen B7
20. Elements which stimulate gene amplification in mammalian cells: Role of recombinogenic sequences/structures and transcriptional activation
21. Severe position effects imposed on a 1 kb mouse whey acidic protein gene promoter are overcome by heterologous matrix attachment regions
22. Dissection of the ability of the chicken lysozyme gene 5′ matrix attachment region to stimulate transgene expression and to dampen position effects
23. Nonrandom distribution of repeated DNA sequences with respect to supercoiled loops and the nuclear matrix
24. Homologous recombination between defective neo genes in mouse 3T6 cells
25. Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination
26. A nuclear DNA attachment element mediates elevated and position-independent gene activity
27. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors
28. Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs
29. High frequency targeting of genes to specific sites in the mammalian genome
30. Partial characterization of nuclear matrix attachment regions from human fibroblast DNA using Alu-Polymerase chain reaction
31. Evaluation of the function of the human apolipoprotein B gene nuclear matrix association regions in transgenic mice
32. Enhancers increase the probability but not the level of gene expression
33. Distinct mouse DNA sequences enable establishment and persistence of plasmid DNA polymers in mouse cells