Expression and purification of ataxin-1 protein

Journal of Neuroscience Methods

Volumn 189, Issue 1, 2010, Pages 30-35

  Husain Ponnampalam, Rhonda ^a   Turnbull, Victor ^a   Tarlac, Volga ^a   Storey, Elsdon ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

Dual affinity purification; Escherichia coli expression; Monoclonal antibody production; Recombinant ataxin 1 protein

Indexed keywords

ATAXIN 1; BUFFER; GLUTAMINE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MONOCLONAL ANTIBODY; POLYGLUTAMINE; PROTEINASE; SOLVENT;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DEGENERATIVE DISEASE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WESTERN BLOTTING; WILD TYPE;

AFFINITY LABELS; AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BIOCHEMISTRY; BIOLOGICAL ASSAY; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ESCHERICHIA COLI; HUMANS; NERVE TISSUE PROTEINS; NUCLEAR PROTEINS; PROTEOMICS; RECOMBINANT FUSION PROTEINS;

EID: 77952547245     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2010.03.008     Document Type: Article

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