A divalent FHA/BRCT-binding mechanism couples the MRE11-RAD50-NBS1 complex to damaged chromatin

EMBO Reports

Volumn 11, Issue 5, 2010, Pages 387-392

  Hari, Flurina J ^a   Spycher, Christoph ^a   Jungmichel, Stephanie ^a   Pavic, Lucijana ^a   Stucki, Manuel ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

Chromatin; DNA double strand breaks; G2 M checkpoint; MDC1; NBS1

Indexed keywords

HISTONE H2AX; MEDIATOR OF THE DNA DAMAGE CHECKPOINT 1; MRE11 PROTEIN; NIBRIN; PROTEIN SERINE THREONINE KINASE; RAD50 PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CELL CYCLE G2 PHASE; CHROMATIN; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; GENE MUTATION; HUMAN; HUMAN CELL; MUTATION; POINT MUTATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; SEQUENCE ANALYSIS; WILD TYPE;

AMINO ACID SEQUENCE; CELL CYCLE PROTEINS; CELL LINE; CHROMATIN; DNA BREAKS, DOUBLE-STRANDED; DNA DAMAGE; DNA REPAIR ENZYMES; DNA-BINDING PROTEINS; G2 PHASE; HUMANS; MITOSIS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR PROTEINS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; STRUCTURE-ACTIVITY RELATIONSHIP; TRANS-ACTIVATORS;

EID: 77951879405     PISSN: 1469221X     EISSN: 14693178     Source Type: Journal    
DOI: 10.1038/embor.2010.30     Document Type: Article

References (22)

1. Becker E, Meyer V, Madaoui H, Guerois R (2006) Detection of a tandem BRCT in Nbs1 and Xrs2 with functional implications in the DNA damage response. Bioinformatics 22: 1289-1292 (Pubitemid 43985262)
2. Cerosaletti KM, Concannon P (2003) Nibrin forkhead-associated domain and breast cancer C-terminal domain are both required for nuclear focus formation and phosphorylation. J Biol Chem 278: 21944-21951 (Pubitemid 36792602)
3. Chapman J, Jackson S (2008) Phospho-dependent interactions between NBS1 and MDC1 mediate chromatin retention of the MRN complex at sites of DNA damage. EMBO Rep 9: 795-801
4. D'Amours D, Jackson SP (2002) The Mre11 complex: at the crossroads of DNA repair and checkpoint signalling. Nat Rev Mol Cell Biol 3: 317-327 (Pubitemid 34619266)
5. Difilippantonio S, Nussenzweig A (2007) The NBS1-ATM connection revisited. Cell Cycle 6: 2366-2370 (Pubitemid 350058689)
6. Difilippantonio S et al (2005) Role of Nbs1 in the activation of the Atm kinase revealed in humanized mouse models. Nat Cell Biol 7: 675-685 (Pubitemid 40975748)
7. Difilippantonio S, Celeste A, Kruhlak MJ, Lee Y, Difilippantonio MJ, Feigenbaum L, Jackson SP, McKinnon PJ, Nussenzweig A (2007) Distinct domains in Nbs1 regulate irradiation-induced checkpoints and apoptosis. J Exp Med 204: 1003-1011 (Pubitemid 46798145)
8. Glover JN, Williams RS, Lee MS (2004) Interactions between BRCT repeats and phosphoproteins: tangled up in two. Trends Biochem Sci 29: 579-585
9. Lou Z, Chini CCS, Minter-Dykhouse K, Chen J (2003) Mediator of DNA damage checkpoint protein 1 regulates BRCA1 localization and phosphorylation in DNA damage checkpoint control. J Biol Chem 278: 13599-13602 (Pubitemid 36799890)
10. Lou Z et al (2006) MDC1 maintains genomic stability by participating in the amplification of ATM-dependent DNA damage signals. Mol Cell 21: 187-200 (Pubitemid 43099935)
11. Lloyd J, Chapman R, Clapperton JA, Haire LF, Hartsuiker E, Li J, Carr AM, Jackson SP, Smerdon SJ (2009) A supramodular FHA/BRCT-repeat architecture mediates Nbs1 adaptor function in response to DNA damage. Cell 139: 100-111
12. Lukas C, Melander F, Stucki M, Falck J, Bekker-Jensen S, Goldberg M, Lerenthal Y, Jackson SP, Bartek J, Lukas J (2004) Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention. EMBO J 23: 2674-2683 (Pubitemid 38988238)
13. Melander F, Bekker-Jensen S, Falck J, Bartek J, Mailand N, Lukas J (2008) Phosphorylation of SDT repeats in the MDC1 N terminus triggers retention of NBS1 at the DNA damage-modified chromatin. J Cell Biol 181: 213-226 (Pubitemid 351574548)
14. Spycher C, Miller ES, Townsend K, Pavic L, Morrice NA, Janscak P, Stewart GS, Stucki M (2008) Constitutive phosphorylation of MDC1 physically links the MRE11-RAD50-NBS1 complex to damaged chromatin. J Cell Biol 181: 227-240 (Pubitemid 351574549)
15. Stewart GS, Wang B, Bignell CR, Taylor AMR, Elledge SJ (2003) MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature 421: 961-966 (Pubitemid 36288029)
16. Stracker TH, Theunissen JW, Morales M, Petrini JH (2004) The Mre11 complex and the metabolism of chromosome breaks: the importance of communicating and holding things together. DNA Repair (Amst) 3: 845-854 (Pubitemid 38997926)
17. Stucki M, Clapperton JA, Mohammad D, Yaffe MB, Smerdon SJ, Jackson SP (2005) MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. Cell 123: 1213-1226 (Pubitemid 41821780)
18. van der Burgt I, Chrzanowska KH, Smeets D, Weemaes C (1996) Nijmegen breakage syndrome. J Med Genet 33: 153-156 (Pubitemid 26056471)
19. Williams RS et al (2009) Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair. Cell 139: 87-99
20. Wu L, Luo K, Lou Z, Chen J (2008) MDC1 regulates intra-S-phase checkpoint by targeting NBS1 to DNA double-strand breaks. Proc Natl Acad Sci USA 105: 11200-11205
21. Xu C, Wu L, Cui G, Botuyan MV, Chen J, Mer G (2008) Structure of a second BRCT domain identified in the Nijmegen breakage syndrome protein Nbs1 and its function in an MDC1-dependent localization of Nbs1 to DNA damage sites. J Mol Biol 381: 361-372
22. Yu X, Chen J (2004) DNA damage-induced cell cycle checkpoint control requires CtIP, a phosphorylation-dependent binding partner of BRCA1 C-terminal domains. Mol Cell Biol 24: 9478-9486 (Pubitemid 39391684)