Comprehensive bioinformatics analysis of cell-free protein synthesis: Identification of multiple protein properties that correlate with successful expression

FASEB Journal

Volumn 24, Issue 4, 2010, Pages 1095-1104

  Kurotani, Atsushi ^a,b   Takagi, Tetsuo ^a   Toyama, Mitsutoshi ^a   Shirouzu, Mikako ^a   Yokoyama, Shigeyuki ^a,c   Fukami, Yasuo ^d   Tokmakov, Alexander A ^a,d  

^a RIKEN   (Japan)

^b Plant Science Center   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

^d KOBE UNIVERSITY   (Japan)

Author keywords

Heterologous expression; Physicochemical protein properties; Rationalization; Recombinant proteins; Structural protein properties

Indexed keywords

CYSTEINE; RECOMBINANT PROTEIN;

ALPHA HELIX; ARTICLE; BETA SHEET; BIOINFORMATICS; CELL FREE SYSTEM; DISULFIDE BOND; HUMAN; HYDROPHOBICITY; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; BIOLOGY; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN MOTIF; PROTEIN TERTIARY STRUCTURE; THEORETICAL MODEL;

AMINO ACID MOTIFS; CELL-FREE SYSTEM; COMPUTATIONAL BIOLOGY; ESCHERICHIA COLI; HUMANS; MODELS, THEORETICAL; PROTEIN BIOSYNTHESIS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 77951633563     PISSN: 08926638     EISSN: 15306860     Source Type: Journal    
DOI: 10.1096/fj.09-139527     Document Type: Article

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