Using an Escherichia coli cell-free extract to screen for soluble expression of recombinant proteins

Journal of Structural and Functional Genomics

Volumn 5, Issue 1-2, 2004, Pages 69-74

  Busso, Didier ^a,c   Kim, Rosalind ^b   Kim, Sung Hou ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c INSTITUT DE GÉNÉTIQUE ET DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

Author keywords

Cell free; Expression; High throughput; Recombinant protein; Structural genomics

Indexed keywords

BACTERIAL PROTEIN; HISTIDINE; MICROORGANISM PROTEIN; PROTEIN ANTIBODY; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL FREE SYSTEM; CONTROLLED STUDY; DOT HYBRIDIZATION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENETIC RECOMBINATION; GENOMICS; HIGH THROUGHPUT SCREENING; IN VIVO STUDY; MICROBIAL DIVERSITY; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; SOLUBILITY; STRUCTURAL GENE; TEMPERATURE DEPENDENCE;

DNA, RECOMBINANT; ESCHERICHIA COLI; GENE EXPRESSION; GENOMICS; OPEN READING FRAMES; PROTEOMICS; RECOMBINANT PROTEINS; SOLUBILITY; TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 3543091016     PISSN: 1345711X     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:JSFG.0000029197.44728.c5     Document Type: Article

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