Antigenotoxic potential of gymnema montanum leaves on DNA damage in human peripheral blood lymphocytes and HL-60 cell line

Environmental and Molecular Mutagenesis

Volumn 51, Issue 4, 2010, Pages 285-293

  Ramkumar, K M ^a   Sankar, L ^a   Manjula, C ^a   Krishnamurthi, K ^b   Saravana Devi, S ^b   Chakrabarti, T ^b   Kalaiselvi, K ^c   Palanivel, M ^c   Rajaguru, P ^a  

^a ANNA UNIVERSITY   (India)

^b NATIONAL ENVIRONMENTAL ENGINEERING RESEARCH INSTITUTE   (India)

^c PSG COLLEGE OF ARTS AND SCIENCE   (India)

Author keywords

Comet assay; Genotoxicity; Gymnema montanum; HL 60 cell line; Human lymphocytes; Total phenolics

Indexed keywords

ASCORBIC ACID; GYMNEMA MONTANUM EXTRACT; HYDROGEN PEROXIDE; LIPOCORTIN 5; MESYLIC ACID METHYL ESTER; PLANT EXTRACT; PLANT MEDICINAL PRODUCT; UNCLASSIFIED DRUG; ANTIMUTAGENIC AGENT; ANTIOXIDANT; HYDROXYBENZOIC ACID DERIVATIVE; PHENOLIC ACID; PROTECTIVE AGENT;

ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; BINDING ASSAY; CELL CYCLE; CELL STRAIN HL 60; COMET ASSAY; DNA DAMAGE; DOSE RESPONSE; DRUG EFFECT; GYMNEMA MONTANUM; HUMAN; HUMAN CELL; IN VITRO STUDY; MEDICINAL PLANT; OXIDATIVE STRESS; PERIPHERAL LYMPHOCYTE; PLANT LEAF; CHEMISTRY; GYMNEMA; LYMPHOCYTE; METABOLISM; MUTAGENESIS;

GYMNEMA MONTANUM;

ANTIMUTAGENIC AGENTS; ANTIOXIDANTS; DNA DAMAGE; GYMNEMA; HL-60 CELLS; HUMANS; HYDROXYBENZOIC ACIDS; LYMPHOCYTES; MUTAGENESIS; PLANT EXTRACTS; PLANT LEAVES; PROTECTIVE AGENTS;

EID: 77951630513     PISSN: 08936692     EISSN: 10982280     Source Type: Journal    
DOI: 10.1002/em.20543     Document Type: Article

References (42)

1. Abaza L, Talorete TPN, Yamada P, Kurita Y, Zarrouk M, Isoda H. 2007. Induction of growth inhibition and differentiation of human leukemia HL-60 cells by a Tunisian Gerboui olive leaf extract. Biosci Biotechnol Biochem 71:60716-1-7. (Pubitemid 47164252)
2. Bandyopadhyay U, Das D, Banerjee KR. 1999. Reactive oxygen species: Oxidative damage and pathogenesis. Curr Sci 77:658-666. (Pubitemid 129558409)
3. Blokhina O, Virolainen E, Fagerstedt KV. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: A review. Ann Bot 91:179-194. (Pubitemid 36117177)
4. Ceriello A. 2006. Oxidative stress and diabetes-associated complications. Endocr Pract 12:60-62.
5. Chakraborty S, Roy M, Bhattacharya RK. 2004. Prevention and repair of DNA damage by selected phytochemicals as measured by single cell gel electrophoresis. J Environ Pathol Toxicol Oncol 23:215-226. (Pubitemid 41737150)
6. Chattopadhyay RR. 1998. Possible mechanism of antihyperglycemic effect of Gymnema sylvestre leaf extract, Part I. Gen Pharmacol 31:495-496. (Pubitemid 28398881)
7. Davies KJ. 2000. Oxidative stress, antioxidant defenses and damage removal, repair and replacement systems. IUBMB Life 50:279-289.
8. Dragsted LO. 2003. Antioxidant actions of polyphenols in humans. Int J Vitam Nutr Res 73:112-119.
9. Duncan BD. 1957. Multiple range tests for correlated and heteroscedastic means. Biometrics 13:359-364.
10. Duthie SJ, Johnson W, Dobson VL. 1997. The effect of dietary flavonoids on DNA damage (strand breaks and oxidised pyrimdines) and growth in human cells. Mutat Res 390:141-151. (Pubitemid 27230219)
11. Faust F, Kassie F, Knasmuller S, Boedecker RH, Mann M, Mersch-Sundermann. 2004. The use of the alkaline comet assay with lymphocytes with human biomonitoring studies. Mutat Res 566:209-229.
12. Halliwell B. 1996. Free radicals, proteins and DNA: Oxidative damage versus redox regulation. Biochem Soc Trans 24:1023-1027. (Pubitemid 26417963)
13. Halliwell B. 1999. Antioxidant defence mechanisms: From the beginning to the end (of the beginning). Free Radic Res 31:261-272. (Pubitemid 29452853)
14. Hensley K, Robinson KA, Gabbita SP, Salsman S, Floyd RA. 2000. Reactive oxygen species, cell signaling, and cell injury. Free Radic Biol Med 28:1456-1462.
15. Hiramoto K, Ojima N, Sako K, Kikugawa K. 1996. Effect of plant phenolics on the formation of the spin-adduct of hydroxyl radical and the DNA strand breaking by hydroxyl radical. Biol Pharm Bull 19:558-563.
16. Ho CT. 1992. Phenolic compounds in food: An overview. In: Huang MT, Ho CT, Lee CY, editors. Phenolic Compounds in Food and their Effects on Health. II. Washington: American Chemical Society. pp 2-7.
17. Hooker JD. 1883. Asclepiadaceae. The Flora of British India, Vol.4, International Book Distributors, Dehradun, India. p 31.
18. Lodha R, Bagga A. 2000. Traditional Indian systems of medicine. Ann Acad Med Singapore 29:37-41.
19. Nakayama T. 1994. Suppression of hydroperoxide-induced cytotoxicity by polyphenols. Can Res 54:1991-1993.
20. Persaud SJ, Al-Majed H, Raman A, Jones PM. 1999. Gymnema sylvestre stimulates insulin release in vitro by increased membrane permeability. J Endocrinol 163:207-212.
21. Pervaiz S, Climent MV. 2002. Hydrogen peroxide-induced apoptosis: Oxidative or reductive stress. Methods Enzymol 352:150-159.
22. Petzold GL, Swenberg JA. 1978. Detection of DNA damage induced in vivo following exposure of rats to carcinogens. Cancer Res 38:1589-1594.
23. Ramkumar KM, Latha M, Ashokkumar N, Pari L, Ananthan R. 2005. Modulation of impaired cholinesterase activity in experimental diabetes: Effect of Gymnema montanum leaf extract. J Basic Clin Physiol Pharmacol 16:17-34.
24. Ramkumar KM, Rajaguru P, Latha M, Ananthan R. 2007. Amelioration of metabolic complications associated with diabetes by Gymnema montanum in alloxan induced rats. In: Govil JN, Singh VK, Siddiqui NT, editors. Recent Progress in Medicinal Plants, Vol.18., Studium Press LLC, Houston, Texas, pp 319-338.
25. Ramkumar KM, Manjula C, Sankar L, Suriyanarayanan S, Rajaguru P. 2009. Potential in vitro antioxidant and protective effects of Gymnema montanum H. on alloxan-induced oxidative damage in pancreatic β-cells, HIT-T15. Food Chem Toxicol 47:2246-2256.
26. Rani G, Kaur K, Wadhwa R, Kaul SC, Nagpal A. 2005. Evaluation of the anti-genotoxicity of leaf extract of Ashwagandha. Food Chem Toxicol 43:95-98.
27. Ruch RJ, Cheng SJ, Klaunig JE. 1989. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogen 10:1003-1008.
28. Santos FV, Colus IM, Silva MA, Vilegas W, Varanda EA. 2006. Assessment of DNA damage by extracts and fractions of Strychnos pseudoquina, a Brazilian medicinal plant with antiulcerogenic activity. Food Chem Toxicol 44:1585-1589.
29. Scalbert A, Johnson IT, Saltmarsh M. 2005a. Polyphenols: Antioxidants and beyond. Am J Clin Nutr 81:215-217.
30. Scalbert A, Manach C, Morand C, Rémésy C, Jiménez L. 2005b. Dietary polyphenols and the prevention of diseases. Crit Rev Food Sci Nutr 45;287-306.
31. Shimizu K, Ozeki M, Ilino A, Nakajyo S, Urakawa N, Atsuchi M. 2001. Structure activity relationships of triterpenoid derivatives extracted from Gymnema inodorum leaves on glucose absorption. Jpn J Pharmacol 86:223-229.
32. Singh NP, McCoy MT, Tice RR, Schneider EL. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184-191.
33. Szeto YT, Collins AR, Benzie IF. 2002. Effects of dietary antioxidants on DNA damage in lysed cells using a modified comet assay procedure. Mutat Res 500:31-38.
34. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. 2000. Single cell gel/comet assay: Guidelines for in vitro and in vivo genotoxicology testing. Environ Mol Mutagen 35:206-221.
35. Tomasetti M, Alleva R, Borghi B, Collins AR. 2001. In vivo supplementation with coenzyme Q10 enhances the recovery of human lymphocytes from oxidative DNA damage. FASEB J 15:1425-1427.
36. Vajravelu E, Bhargavan P. 1983. Rare and endemic species re-collected after fifty years or more from South India. In: Jain SK, Raw RR, editors. An Assessment of Threatened Plants of India (Proceedings of the seminar held at Dehradun). Howrah: BSI, Botanic Garden. pp 14-17.
37. Vichnevetskaia KD, Roy DN. 2001. Oxidative stress and antioxidative defence with an emphasis on plants antioxidants. Environ Rev 7:31-51.
38. Xie JT, Wang A, Mehendale S, Wu J, Aung HH, Dey L, Qiu S, Yuan CS. 2003. Anti-diabetic effects of Gymnema yunnanense extract. Pharmacol Res 47:323-329.
39. Yen GC, Duh PD, Lin CW. 2003. Effects of resveratrol and 4-hexylresorcinol on hydrogen peroxide-induced oxidative DNA damage in human lymphocytes. Free Rad Res 37:509-514.
40. Yoshizawa Y, Fukiya Y, Izumi Y, Hata K, Iwashita J, Murofushi N, Abe T. 2002. Induction of apoptosis with an extract of Actinidia polygama fruit in the promyelocytic leukemia cell line HL-60. J Health Sci 48:303-309.
41. Zheng W, Wang SY. 2001. Antioxidant activity and phenolic compounds in selected herbs. J Agri Food Chem 49:5165-5170.
42. Zinoveva VN, Spasov AA. 2004. DNA protective activity of natural and synthetic antioxidants. Biomed Khim 50:231-242.