Establishment of a methodology for investigating protectants against ethanol-induced hepatotoxicity

Food and Chemical Toxicology

Volumn 48, Issue 5, 2010, Pages 1145-1151

  Ruan, Xueqing ^a   Shen, Chong ^a   Meng, Qin ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

3D cultured hepatocytes; Ethanol; Hepatotoxicity; Silibinin; Steatosis

Indexed keywords

ALCOHOL; CYTOCHROME P450 2E1; FENOFIBRATE; GLUTATHIONE; LIPID; LIVER PROTECTIVE AGENT; MALONALDEHYDE; NANO SILIBININ; REACTIVE OXYGEN METABOLITE; SILIBININ; TRIACYLGLYCEROL; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; DRUG BIOAVAILABILITY; DRUG SCREENING; ENZYME INDUCTION; EVAPORATION; IN VITRO STUDY; LIPID STORAGE; LIVER CELL CULTURE; LIVER TOXICITY; MALE; METHODOLOGY; MONOLAYER CULTURE; NONHUMAN; RAT; SENSITIVITY ANALYSIS;

ANIMALS; ANTILIPEMIC AGENTS; ANTIOXIDANTS; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CELLS, CULTURED; CHEMOPREVENTION; DRUG-INDUCED LIVER INJURY; ETHANOL; HEPATOCYTES; MALE; MALONDIALDEHYDE; MILK THISTLE; NANOPARTICLES; NANOTECHNOLOGY; OXIDATIVE STRESS; PLANT EXTRACTS; PROCETOFEN; RATS; RATS, SPRAGUE-DAWLEY; REACTIVE OXYGEN SPECIES; SILYMARIN;

EID: 77951621434     PISSN: 02786915     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fct.2010.02.001     Document Type: Article

References (43)

1. Aroor A.R., Custer G.W., Weng Y.I., Lee Y.J., Shukla S.D. Phosphotidylethanol mimics ethanol modulation of p42/44 mitogen-activated protein kinase signalling in hepatocytes. Alcohol Alcohol. 2002, 37:534-539.
2. Bradbury M.W., Berk P.D. Lipid metabolism in hepatic steatosis. Clin. Liver Dis. 2004, 8:639-671. xi.
3. Castilla R., Gonzalez R., Fouad D., Fraga E., Muntane J. Dual effect of ethanol on cell death in primary culture of human and rat hepatocytes. Alcohol Alcohol. 2004, 39:290-296.
4. Cobreros A., Sainz L., Lasheras B., Cenarruzabeitia E. Hepatotoxicity of ethanol: protective effect of calcium channel blockers in isolated hepatocytes. Liver 1997, 17:76-82.
5. Comoglio A., Tomasi A., Malandrino S., Poli G., Albano E. Scavenging effect of silipide, a new silybin-phospholipid complex, on ethanol-derived free radicals. Biochem. Pharmacol. 1995, 50:1313-1316.
6. Conti M., Malandrino S., Magistretti M.J. Protective activity of silipide on liver damage in rodents. Jpn. J. Pharmacol. 1992, 60:315-321.
7. Donato M.T., Lahoz A., Jimenez N., Perez G., Serralta A., Mir J., Castell J.V., Gomez-Lechon M.J. Potential impact of steatosis on cytochrome P450 enzymes of human hepatocytes isolated from fatty liver grafts. Drug Metab. Dispos. 2006, 34:1556-1562.
8. Donohue T.M. Alcohol-induced steatosis in liver cells. World J. Gastroenterol. 2007, 13:4974-4978.
9. El-Assal O., Hong F., Kim W.H., Radaeva S., Gao B. IL-6-deficient mice are susceptible to ethanol-induced hepatic steatosis: IL-6 protects against ethanol-induced oxidative stress and mitochondrial permeability transition in the liver. Cell. Mol. Immunol. 2004, 1:205-211.
10. Ernstgard L. Influence of gender on the metabolism of alcohols in human saliva in vitro. Arch. Oral. Biol. 2009, 54:737-742.
11. Gazak R., Walteroa D., Kren V. Silybin and silymarin, niw and emering applications in medicine. Curr. Med. Chem. 2007, 14:315-338.
12. Jasmund I., Schwientek S., Acikgoz A., Langsch A., Machens H.G., Bader A. The influence of medium composition and matrix on long-term cultivation of primary porcine and human hepatocytes. Biomol. Eng. 2007, 24:59-69.
13. Katrusiak A.E., Paterson P.G., Kamencic H., Shoker A., Lyon A.W. Pre-column derivatization high-performance liquid chromatographic method for determination of cysteine, cysteinyl-glycine, homocysteine and glutathione in plasma and cell extracts. J. Chromatogr. B 2001, 758:207-212.
14. Kimura T., Kawasaki Y., Okumura F., Sone T., Natsuki R., Isobe M. Ethanol-induced expression of glutamate-cysteine ligase catalytic subunit gene is mediated by NF-κB. Toxicol. Lett. 2009, 185:110-115.
15. Lee M.S., Kim M.S., Park S.Y., Kang C.W. Effects of betaine on ethanol-stimulated secretion of IGF-I and IGFBP-1 in rat primary hepatocytes: involvement of p42/44 MAPK activation. World J. Gastroenterol. 2006, 12:1718-1722.
16. Lewerenz V., Hanelt S., Nastevska C., El-Bahay C., Rohrdanz E., Kahl R. Antioxidants protect primary rat hepatocyte cultures against acetaminophen-induced DNA strand breaks but not against acetaminophen-induced cytotoxicity. Toxicology 2003, 191:179-187.
17. Lieber C.S. Alcoholic fatty liver: its pathogenesis and mechanism of progression to inflammation and fibrosis. Alcohol 2004, 34:9-19.
18. Liu L.G., Yan H., Yao P., Zhang W., Zou L.J., Song F.F., Li K., Sun X.F. CYP2E1-dependent hepatotoxicity and oxidative damage after ethanol administration in human primary hepatocytes. World J. Gastroenterol. 2005, 11:4530-4535.
19. Momeny M., Khorramizadeh M.R., Ghaffari S.H., Yousefi M., Yekaninejad M.S., Esmaeili R., Jahanshiri Z., Nooridaloii M.R. Effects of silibinin on cell growth and invasive properties of a human hepatocellular carcinoma cell line, HepG-2, through inhibition of extracellular signal-regulated kinase 1/2 phosphorylation. Eur. J. Pharmacol. 2008, 591:13-20.
20. Morazzoni P., Montalbetti A., Malandrino S., Pifferi G. Comparative pharmacokinetics of silipide and silymarin in rats. Eur. J. Drug Metab. Pharmacokinet. 1993, 18:289-297.
21. Moulisova V., Srbova M., Jedlickova O., Sebestian J., Jegorov A. Silybin reduces lipid peroxidation of rat hepatocyte membrane caused by cyclosporin A. Biochemistry (Mosc) 2006, 71:1110-1114.
22. Nagata K., Suzuki H., Sakaguchi S. Common pathogenic mechanism in development progression of liver injury caused by non-alcoholic or alcoholic steatohepatitis. J. Toxicol. Sci. 2007, 32:453-468.
23. Niemela O., Parkkila S., Juvonen R.O., Viitala K., Gelboin H.V., Pasanen M. Cytochromes P450 2A6, 2E1, and 3A and production of protein-aldehyde adducts in the liver of patients with alcoholic and non-alcoholic liver diseases. J. Hepatol. 2000, 33(6):893-901.
24. Norikura T., Kojima-Yuasa A., Opare Kennedy D., Matsui-Yuasa I. Protective effect of gamma-aminobutyric acid (GABA) against cytotoxicity of ethanol in isolated rat hepatocytes involves modulations in cellular polyamine levels. Amino Acids 2007, 32:419-423.
25. Qu B., Li Q.T., Wong K.P., Tan T.M., Halliwell B. Mechanism of clofibrate hepatotoxicity: mitochondrial damage and oxidative stress in hepatocytes. Free Radic. Biol. Med. 2001, 31:659-669.
26. Raucy J.L., Lasker J., Ozaki K., Zoleta V. Regulation of CYP2E1 by ethanol and palmitic acid and CYP4A11 by clofibrate in primary cultures of human hepatocytes. Toxicol. Sci. 2004, 79:233-241.
27. Robin M.A., Sauvage I., Grandperret T., Descatoire V., Pessayre D., Fromenty B. Ethanol increases mitochondrial cytochrome P450 2E1 in mouse liver and rat hepatocytes. FEBS Lett. 2005, 579:6895-6902.
28. Rodeiro I., Donato M.T., Martinez I., Hernandez I., Garrido G., Gonzalez-Lavaut J.A., Menendez R., Laguna A., Castell J.V., Gomez-Lechon M.J. Potential hepatoprotective effects of new Cuban natural products in rat hepatocytes culture. Toxicol. In Vitro 2008, 22:1242-1249.
29. Sachan D.S., Yatim A.M., Daily J.W. Comparative effects of dietary corn oil, safflower oil, fish oil and palm oil on metabolism of ethanol and carnitine in the rat. J. Am. Coll. Nutr. 2002, 21:233-238.
30. Sergent O., Pereira M., Belhomme C., Chevanne M., Huc L., Lagadic-Gossmann D. Role for membrane fluidity in ethanol-induced oxidative stress of primary rat hepatocytes. J. Pharmacol. Exp. Ther. 2005, 313:104-111.
31. Shen C., Zhang G., Qiu H., Meng Q. Acetaminophen-induced hepatotoxicity of gel entrapped rat hepatocytes in hollow fibers. Chem. Biol. Interact. 2006, 162:53-61.
32. Shen C., Zhang G.L., Meng Q. An in vitro model for long-term hepatotoxicity testing utilizing rat hepatocytes entrapped in micro-hollow fiber reactor. Biochem. Eng. J. 2007, 34:267-272.
33. Shimada M., Liu L., Nussler N., Jonas S., Langrehr J.M., Ogawa T., Kaminishi M., Neuhaus P., Nussler A.K. Human hepatocytes are protected from ethanol-induced cytotoxicity by DADS via CYP2E1 inhibition. Toxicol. Lett. 2006, 163:242-249.
34. Tsutsumi M., Takase S. Effect of fenofibrate on fatty liver in rats treated with alcohol. Alcohol. Clin. Exp. Res. 2001, 25:75S-79S.
35. van Pelt J.F., Verslype C., Crabbe T., Zaman Z., Fevery J. Primary human hepatocytes are protected against prolonged and repeated exposure to ethanol by silibinin-dihemisuccinate. Alcohol Alcohol. 2003, 38:411-414.
36. Vanhaecke T., Lindros K.O., Oinonen T., Coecke S., DeBast G., Phillips I.R., Shephard E.A., Vercruysse A., Rogiers V. Effect of ethanol on the expression of hepatic glutathione S-transferase: an in vivo/in vitro study. Biochem. Pharmacol. 2000, 60:1491-1496.
37. Vereckei A., Blazovics A., Gyorgy I., Feher E., Toth M., Szenasi G., Zsinka A., Foldiak G., Feher J. The role of free radicals in the pathogenesis of amiodarone toxicity. J. Cardiovasc. Electrophysiol. 1993, 4:161-177.
38. Wang H.Z., Chang C.H., Lin C.P., Tsai M.C. Using MTT viability assay to test the cytotoxicity of antibiotics and steroid to cultured porcine corneal endothelial cells. J. Ocul. Pharmacol. Ther. 1996, 12:35-43.
39. Wong S.H., Knight J.A., Hopfer S.M., Zaharia O., Leach C.N., Sunderman F.W. Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehyde-thiobarbituric acid adduct. Clin. Chem. 1987, 33:214-220.
40. Woodcroft K.J., Novak R.F. Xenobiotic-enhanced expression of cytochromes P450 2E1 and 2B in primary cultured rat hepatocytes. Drug Metab. Dispos. 1998, 26:372-378.
41. Wu D.Q., Zhang G.L., Shen C., Zhao Q., Li H., Meng Q. Evaluation of diffusion in gel entrapment cell culture within hollow fibers. World J. Gastroenterol. 2005, 11:1599-1604.
42. Yao P., Nussler A., Liu L., Hao L., Song F., Schirmeier A., Nussler N. Quercetin protects human hepatocytes from ethanol-derived oxidative stress by inducing heme oxygenase-1 via the MAPK/Nrf2 pathways. J. Hepatol. 2007, 47:253-261.
43. Yin H.Q., Kim Y.C., Chung Y.S., Kim Y.C., Shin Y.K., Lee B.H. Honokiol reverses alcoholic fatty liver by inhibiting the maturation of sterol regulatory element binding protein-1c and the expression of its downstream lipogenesis genes. Toxicol. Appl. Pharmacol. 2009, 236:124-130.