Effects of culture conditions on N-glycolylneuraminic acid (Neu5Gc) content of a recombinant fusion protein produced in CHO cells

Biotechnology and Bioengineering

Volumn 105, Issue 6, 2010, Pages 1048-1057

  Borys, Michael C ^a   Dalal, Nimish G ^a   Abu Absi, Nicholas R ^a   Khattak, Sarwat F ^a   Jing, Ying ^a   Xing, Zizhuo ^a   Li, Zheng Jian ^a  

^a BRISTOL MYERS SQUIBB COMPANY   (United States)

Author keywords

Glycoprotein; Mammalian cell culture; N acetylglycolylneuraminic acid; Neu5Gc; Sialic acid

Indexed keywords

CHO CELL; CULTURE CONDITIONS; CULTURE TEMPERATURE; EXPONENTIAL GROWTH PHASE; FUSION PROTEINS; GLYCOSYLATED; IMMUNOGENICITY; IN-PROCESS; MAMMALIAN CELL CULTURE; N-ACETYLNEURAMINIC ACID; N-GLYCOLYLNEURAMINIC ACID; NEU5GC; PH CONTROL; PRACTICAL IMPORTANCE; PROCESS DEVELOPMENT; RECOMBINANT GLYCOPROTEINS; RECOMBINANT PROTEIN; SIALIC ACIDS; SODIUM BUTYRATE; SODIUM CARBONATE; SODIUM HYDROXIDES; STATIONARY PHASE; TEMPERATURE SHIFT;

ACIDS; ANIMAL CELL CULTURE; CARBOXYLIC ACIDS; GLYCOPROTEINS; LIGANDS; MAMMALS; PROCESS CONTROL; PROTEINS; SODIUM;

CELL CULTURE;

BUTYRIC ACID; HYBRID PROTEIN; N ACETYLNEURAMINIC ACID; N GLYCOLOYLNEURAMINIC ACID; SIALIC ACID; SODIUM CARBONATE; SODIUM HYDROXIDE;

ANIMAL CELL; ARTICLE; CARBON DIOXIDE TENSION; CELL CULTURE; CONTROLLED STUDY; GROWTH; NONHUMAN; PROTEIN EXPRESSION; TEMPERATURE SENSITIVITY;

ANIMALS; BIOREACTORS; BUTYRATES; CARBON DIOXIDE; CARBONATES; CELL COUNT; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; GLYCOPROTEINS; N-ACETYLNEURAMINIC ACID; NEURAMINIC ACIDS; RECOMBINANT FUSION PROTEINS; SODIUM HYDROXIDE; TEMPERATURE;

MAMMALIA;

EID: 77951587072     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22644     Document Type: Article

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