Enumeration of coagulase and thermonuclease-positive Staphylococcus spp. in raw milk and fresh soft cheese: An evaluation of Baird-Parker agar, Rabbit Plasma Fibrinogen agar and the Petrifilm™ Staph Express count system

Food Microbiology

Volumn 27, Issue 4, 2010, Pages 447-452

  Viçosa, Gabriela Nogueira ^a   Moraes, Paula Mendonça ^a   Yamazi, Anderson Keizo ^a   Nero, Luís Augusto ^a  

^a FEDERAL UNIVERSITY OF VIÇOSA   (Brazil)

Author keywords

Coagulase; Culture media; Enumeration; Staphylococcus; Thermonuclease

Indexed keywords

AGAR; COAGULASE; MICROCOCCAL NUCLEASE;

ANIMAL; ARTICLE; BACTERIAL COUNT; CHEESE; CHEMISTRY; CULTURE MEDIUM; ENZYMOLOGY; FOOD CONTAMINATION; FOOD CONTROL; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MILK; STAPHYLOCOCCUS;

AGAR; ANIMALS; BACTERIOLOGICAL TECHNIQUES; CHEESE; COAGULASE; COLONY COUNT, MICROBIAL; CULTURE MEDIA; FOOD CONTAMINATION; FOOD MICROBIOLOGY; HUMANS; MICROCOCCAL NUCLEASE; MILK; STAPHYLOCOCCUS;

ORYCTOLAGUS CUNICULUS; STAPHYLOCOCCUS;

EID: 77951022427     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2009.12.007     Document Type: Article

References (31)

1. Araújo V.S., Pagliares V.A., Queiroz M.L.P., and Freitas-Almeida A.C. Occurrence of Staphylococcus and enteropathogens in soft cheese commercialized in the city of Rio de Janeiro. Braz. J. Appl. Microbiol. 92 (2002) 1172-1177
2. Baird-Parker A.C. An improved diagnostic and selective medium for isolating coagulase positive staphylococci. J. Appl. Bacteriol. 25 (1962) 12-19
3. Beckers H.J., van Leusden F.M., Bindschedler O., and Guerraz D. Evaluation of a pour plate system with rabbit plasma-bovine plasma-agar for the enumeration of Staphylococcus aureus in food. Can. J. Microbiol. 30 (1984) 470-474
4. Beloti V., Barros M.A.F., Nero L.A., Pachemshy J.A.S., Santana E.H.W., and Franco B.D.G.M. Quality of pasteurized milk influences the performance of ready-to-use systems for enumeration of aerobic microorganisms. Int. Dairy J. 12 (2002) 413-418
5. Boari C.A., Piccoli-Valle R.H., Nascimento A.R., and Alcântara E.M.C. Ocorrência de cepas de estafilococos coagulase positiva formadoras de colônias atípicas em ágar Baird-Parker em queijos maturados. B. CEPPA 20 (2002) 347-354
6. de Buyser M.L., Audinet N., Delbart M.O., Maire M., and Françoise F. Comparison of selective culture media to enumerate coagulase-positive staphylococci in cheeses made from raw milk. Food Microbiol. 15 (1998) 339-346
7. de Buyser M.L., Lombard B., Schulten S.M., In't Veld P.H., Scotter S.L., Rollier P., and Lahellec C. Validation of EN ISO standard methods 6888 part 1 and part 2: 1999-Enumeration of coagulase-positive staphylococci in foods. Int. J. Food Microbiol. 83 (2003) 185-194
8. Capita R., Alonso-Calleja K.C., Moreno B., and García-Fernandez M.C. Assessment of Baird-Parker agar as screening test for determination of Staphylococcus aureus in poultry meat. J. Microbiol. 39 (2001) 321-325
9. do Carmo L.S., Dias R.S., Linardi V.R., de Sena M.J., dos Santos D.A., de Faria M.E., Pena E.C., Jett M., and Heneine L.G. Food poisoning due to enterotoxigenic strains of Staphylococcus present in Minas cheese and raw milk in Brazil. Food Microbiol. 19 (2002) 9-14
10. Freitas R., Nero L.A., and Carvalho A.F. Technical note: enumeration of mesophilic aerobes in milk: evaluation of standard official protocols and Petrifilm aerobic count plates. J. Dairy Sci. 92 (2009) 3069-3073
11. Ingham S.C., Becker K.L., and Fanslau M.A. Comparison of the Baird-Parker agar and 3M Petrifilm Staph Express count plate methods for enumeration of Staphylococcus aureus in naturally and artificially contaminated foods. J. Food Prot. 66 (2003) 2151-2155
12. ISO - International Organisation of Standardisation. ISO 6888-1. Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Enumeration of Coagulase Positive Staphylococci (Staphylococcus aureus and Other Species): Part 1. Technique Using Baird-Parker Agar Medium (1999), International Organisation of Standardisation, Geneva
13. ISO - International Organisation of Standardisation. ISO 6888-2. Microbiology of Food and Animal Feeding Stuffs - Horizontal Method for the Enumeration of Coagulase Positive Staphylococci (Staphylococcus aureus and Other Species): Part 1. Technique using Rabbit Plasma Fibrinogen agar medium (1999), International Organisation of Standardisation, Geneva
14. Lancette G.A., and Bennett R.W. In: Downes F.C., and Ito K. (Eds). Compendium of Methods for the Microbiological Examination of Foods. fourth ed. (2001), American Public Health Press 387-403
15. Miwa N., Kawamura A., Masuda T., and Akiyama M. An outbreak of food poisoning due to egg yolk reaction-negative Staphylococcus aureus. Int. J. Food Microbiol. 64 (2001) 361-366
16. Moraes P.M., Viçosa G.N., Yamazi A.K., Ortolani M.B.T., and Nero L.A. Foodborne pathogens and microbiological characteristics of raw milk soft cheese produced and on retail sale in Brazil. Foodborne Pathog. Dis. 6 (2009) 245-249
17. Nema V., Agrawal R., Kamboj D.V., Goel A.K., and Singh L. Isolation and characterization of heat resistant enterotoxigenic Staphylococcus aureus from a food poisoning outbreak in Indian subcontinent. Int. J. Food Microbiol. 117 (2007) 29-35
18. Nero L.A., Beloti V., Barros M.A.F., Ortolani M.B.T., Tamanini R., and Franco B.D.G.M. Comparison of Petrifilm aerobic count plates and de Man-Rogosa-Sharpe agar for enumeration of lactic acid bacteria. J. Rapid Methods Autom. Microbiol. 14 (2006) 249-257
19. Nero L.A., Rodrigues L.A., Viçosa G.N., and Ortolani M.B.T. Performance of Petrifilm aerobic count plates on enumeration of lactic acid bacteria in fermented milks. J. Rapid Methods Autom. Microbiol. 16 (2008) 132-139
20. Normanno G., Firinu A., Virgilio S., Mula G., Dambrosio A., Poggiu A., Decastelli L., Mioni R., Scuota S., Bolzoni G., Di Giannatale E., Salinetti A.P., La Salandra G., Bartoli M., Zuccon F., Pirino T., Sias S., Parisi A., Quaglia N.C., and Celano G.V. Coagulase-positive Staphylococci and Staphylococcus aureus in food products marketed in Italy. Int. J. Food Microbiol. 98 (2005) 73-79
21. Ortolani M.B.T., Viçosa G.N., Beloti V., and Nero L.A. Screening and enumeration of lactic acid bacteria in milk using three different culture media in Petrifilm™ Aerobic count plates and conventional pour plate methodology. J. Dairy Res. 74 (2007) 387-391
22. Rosec J.P., Guiraud J.P., Dalet C., and Richard N. Enterotoxin production by staphylococci isolated from foods in France. Int. J. Food Microbiol. 35 (1997) 213-221
23. Sant'Ana A.S., and Azeredo D.R.P. Comparação entre o sistema Petrifilm RSA™ e a metodologia convencional para a enumeração de estafilococos coagulase positiva em alimentos. Cienc. Tecnol. Aliment. 25 (2005) 531-535
24. Sawhney D. The toxicity of potassium tellurite to Staphylococcus aureus in rabbit plasma fibrinogen agar. J. Appl. Bacteriol. 61 (1986) 149-155
25. Schoeller N.P., and Ingham S.C. Comparison of the Baird-Parker agar and 3M™ Petrifilm™ rapid S. aureus count plate methods for detection and enumeration of Staphylococcus aureus. Food Microbiol. 18 (2001) 581-587
26. Sharpe A.N., and Jackson A.K. Comparison of poured and surface inoculated plates of Baird-Parker's medium for enumerating Staphylococcus aureus in foods. J. Appl. Bacteriol. 35 (1972) 681-684
27. Silva W.P., Destro M.T., Landgraf M., and Franco B.D.G.M. Biochemical characteristics of typical and atypical Staphylococcus aureus in mastitic milk and environmental samples of Brazilian dairy farms. Braz. J. Microbiol. 31 (2000) 103-106
28. Stadhouders J., Hassing F., and Vanaalst-Vanmaren N.O. A pour-plate method for the detection and enumeration of coagulase-positive Staphylococcus aureus in the Baird-Parker medium without egg yolk. Neth. Milk Dairy J. 30 (1976) 222-229
29. Tassinari A.R., Noda P.K., Franco B.D.G.M., Landgraf M., and Destro M.T. An evaluation of 3M Petrifilm Staph Express count system for enumerating coagulase-positive Staphylococcus. Food Qual. (2006) 54-59 Dec-Jan
30. Veras J.F., do Carmo L.S., Tong L.C., Shupp J.W., Cummings C., dos Santos D.A., Cerqueira M.M.O.P., Cantini A., Nicoli J.R., and Jett M. A study of the enterotoxigenicity of coagulase-negative and coagulase-positive staphylococcal isolates from food poisoning outbreaks in Minas Gerais, Brazil. Int. J. Infect. Dis. 12 (2008) 410-415
31. Wehr H.M., and Frank J.F. Staphylococcus aureus direct plate count. Standard Methods for the Examination of Dairy Products. seventeenth ed. (2004), American Public Health Press, Washington D.C 132-135