Galectin-1 as a fusion partner for the production of soluble and folded human β-1,4-galactosyltransferase-T7 in E. coli

Biochemical and Biophysical Research Communications

Volumn 394, Issue 3, 2010, Pages 679-684

  Pasek, Marta ^a   Boeggeman, Elizabeth ^a   Ramakrishnan, Boopathy ^a   Qasba, Pradman K ^a  

^a SAIC FREDERICK INC   (United States)

Author keywords

Fusion tags; Galectins; Glycosyltransferases; MBP; Protein expression in E. coli; Protein folding

Indexed keywords

AGAROSE; BETA 1,4 GALACTOSYLTRASFERASE T7; CARBOHYDRATE BINDING PROTEIN; GALACTOSE; GALACTOSYLTRANSFERASE; GALECTIN; HISTIDINE; HYBRID PROTEIN; LACTOSE; MALTOSE BINDING PROTEIN; PROTEOGLYCAN; RECOMBINANT PROTEIN; SERINE; TETRASACCHARIDE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE; XYLOSE;

AMINO ACID SEQUENCE; ARTICLE; CARBOHYDRATE SYNTHESIS; CELL INCLUSION; CIRCULAR DICHROISM; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENE INSERTION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN CLEAVAGE; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FOLDING; PROTEIN PURIFICATION; SOLUBILITY;

CIRCULAR DICHROISM; ESCHERICHIA COLI; GALACTOSYLTRANSFERASES; GALECTIN 1; HUMANS; PROTEIN BIOSYNTHESIS; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SPECTROPHOTOMETRY, ULTRAVIOLET;

ESCHERICHIA COLI; MAMMALIA;

EID: 77950629246     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.03.051     Document Type: Article

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