Studies on the metal binding sites in the catalytic domain of β1,4-galactosyltransferase

Glycobiology

Volumn 12, Issue 7, 2002, Pages 395-407

  Boeggeman, Elizabeth ^a,b   Qasba, Pradman K ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b SAIC FREDERICK INC   (United States)

Author keywords

Aspartate motifs; Bovine 4Gal T1; Catalytic domain; Metal binding sites; Substrate binding

Indexed keywords

ALANINE; ASPARAGINE; BETA 1,4 GALACTOSYLTRANSFERASE; CALCIUM ION; COBALT; GALACTOSYLTRANSFERASE; GLUTAMINE; HISTIDINE; LIGAND; MANGANESE; METAL; METHIONINE; UNCLASSIFIED DRUG; N ACETYLLACTOSAMINE SYNTHASE;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME MECHANISM; INHIBITION KINETICS; METAL BINDING; NONHUMAN; PRIORITY JOURNAL; RNA TRANSLATION; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; ANIMAL; CATTLE; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; HUMAN; KINETICS; METABOLISM; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY;

BOVINAE;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CATALYTIC DOMAIN; CATTLE; HUMANS; KINETICS; METALS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; N-ACETYLLACTOSAMINE SYNTHASE; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0036628807     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/cwf045     Document Type: Article

References (48)

1. A family of human beta4-galactosyltransferases. Cloning and expression of two novel UDP-galactose:beta-N-acetylglucosamine beta1, 4-galactosyltransferases, beta4Gal-T2 and beta4Gal-T3
2. Cloning and expression of a proteoglycan UDP-galactose: Beta-xylose beta1, 4-galactosyltransferase I. A seventh member of the human beta4-galactosyltransferase gene family
3. Identification and characterization of large galactosyltransferase gene families: Galactosyltransferases for all functions
4. Analysis of the substrate binding sites of human galactosyltransferase by protein engineering
5. Current protocols in molecular biology
6. Crystal structure of a complex between Serratia marcescens metallo-protease and an inhibitor from Erwinia chrysanthemi
7. The kinetic mechanism of bovine milk galactosyltransferase. The role of alpha-lactalbumin
8. Identification of three sequence regions, two of which contain DXD motifs involved in the catalytic activity of β-1, 4 galactosyltransferase
9. Functional domains of bovine beta-1, 4 galactosyltransferase
10. Expression of deletiontion constructs of bovine beta-1, 4-galactosyltransferase in Escherichia coli: Importance of Cys134 for its activity
11. Sequence-function relationships of prokaryotic and eukaryotic galactosyltransferases
12. A common motif of eukaryotic glycosyltransferases is essential for the enzyme activity of large clostridial cytotoxins
13. Ultraviolet photoinactivation of galactosyltransferase. Protection by substrates
14. Cloning of cDNA encoding the membrane-bound form of bovine beta 1, 4-galactosyltransferase Eur
15. Crystal structures of the bovine beta4galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose
16. Circular dichroism changes in galactosyltransferase upon substrate binding
17. A model of photoprobe docking with beta1, 4-galactosyltransferase identifies a possible carboxylate involved in glycosylation steps
18. Glycosyltransferases in analysis of oligosaccharide structure and function
19. Identification that KfiA, a protein essential for the biosynthesis of the Escherichia coli K5 capsular polysaccharide, is an alpha -UDP-GlcNAc glycosyltransferase. The formation of a membrane-associated K5 requires KfiA, KfiB, and KfiC
20. Vibrational circular dichroism spectroscopy of selected oligopeptide conformations
21. Some kinetic properties of human-milk galactosyl transferase
22. The expanding beta 4-galactosyltransferase gene family: Messages from the databanks
23. Expression of bovine beta-1, 4-galactosyltransferase cDNA in COS-7 cells
24. Rack-induced metal binding vs. flexibility: Met121His azurin crystal structures at different pH
25. Studies on galactosyltransferase. Kinetic effects of lactalbumin with N-acetylglucosamine and glucose as galactosyl group acceptors
26. Studies on galactosyltransferase. Kinetic investigations with N-acetylglucosamine as the galactosyl group acceptor
27. Cloning and sequencing of cDNA of bovine N-acetylglucosamine (beta 1-4) galactosyltransferase
28. Purification, cDNA cloning, and expression of UDP-Gal: Glucosylceramide beta-1, 4-galactosyltransferase from rat brain
29. Active site of bovine galactosyltransferase: Kinetic and fluorescence studies
30. Human homolog of Caenorhabditis elegans sqv-3 gene is galactosyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans
31. Glycosyltransferases. Structure, localization, and control of cell type-specific glycosylation
32. The preparation and characterization of two forms of bovine galactosyl transferase
33. Metal ion activation of galactosyltransferase
34. Crystal structure of lactose synthase reveals a large conformational change in its catalytic component, the beta1, 4-galactosyltransferase-I
35. Crystal structure of β1, 4-galactosyl-transferase complex with UDP-Gal reveals an oligosaccharide acceptor-binding site
36. Alpha-lactalbumin (LA) stimulates milk beta-1, 4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc
37. Bovine beta 1-4-galactosyltransferase: Two sets of mRNA transcripts encode two forms of the protein with different amino-terminal domains. In vitro translation experiments demonstrate that both the short and the enzyme are type II membrane-bound glycoproteins
38. Molecular cloning of a human cDNA encoding beta-1, 4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc beta-1, 4-galactosyltransferase
39. Differential effect of alpha-lactalbumin on beta-1, 4-galactosyltransferase IV activities
40. Cloning of a novel member of the UDP-galactose:beta-N-acetylglucosamine beta1, 4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis
41. Bovine galactosyltransferase: Identification of a clone by direct immunological screening of a cDNA expression library
42. Four critical aspartic acid residues potentially involved in the catalytic mechanism of Escherichia coli K-12 WaaR
43. Conserved structural regions involved in the catalytic mechanism of Escherichia coli K-12 WaaO (RfaI)
44. Glycosyl group transfer
45. X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: Catalytic mechanism and a new protein superfamily
46. Activity of the yeast MNN1 alpha-1, 3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases
47. Secreted fringe-like signaling molecules may be glycosyltransferases
48. Role of a conserved acidic cluster in bovine beta1, 4 galactosyltransferase-1 probed by mutagenesis of a bacterially expressed recombinant enzyme