Generic approach for the generation of stable humanized single-chain Fv fragments from rabbit monoclonal antibodies

Journal of Biological Chemistry

Volumn 285, Issue 12, 2010, Pages 9054-9066

  Borras, Leo ^a   Gunde, Tea ^a   Tietz, Julia ^a   Bauer, Ulrich ^a   Hulmann Cottier, Valérie ^a   Grimshaw, John P A ^a   Urech, David M ^a  

^a ESBATech AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY FRAGMENT; BINDING AFFINITIES; BIOPHYSICAL PROPERTIES; COMPLEMENTARITY-DETERMINING REGIONS; GENERIC APPROACH; MELTING TEMPERATURES; PHARMACOKINETIC PROPERTIES; PRODUCTION YIELD; REFOLDING PROCESS; SINGLE-CHAIN FV; VARIABLE DOMAIN;

BINDING ENERGY; GRAFTING (CHEMICAL); MONOCLONAL ANTIBODIES; PRODUCTION; SURFACE PLASMON RESONANCE;

SCAFFOLDS;

MONOCLONAL ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TUMOR NECROSIS FACTOR INHIBITOR; VASCULOTROPIN INHIBITOR; VASCULOTROPIN RECEPTOR 2; TUMOR NECROSIS FACTOR ALPHA; VASCULOTROPIN A;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; DRUG STABILITY; DRUG SYNTHESIS; HUMAN; HUMAN CELL; MELTING POINT; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN MOTIF; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RABBIT; RECEPTOR BINDING; REPRODUCIBILITY; THERMOSTABILITY; ANIMAL; CHEMISTRY; CYTOLOGY; ENDOTHELIUM CELL; ESCHERICHIA COLI; HYBRIDOMA; IMMUNOGLOBULIN VARIABLE REGION; KINETICS; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING;

ORYCTOLAGUS CUNICULUS;

ANIMALS; ANTIBODIES, MONOCLONAL; COMPLEMENTARITY DETERMINING REGIONS; ENDOTHELIAL CELLS; ESCHERICHIA COLI; HUMANS; HYBRIDOMAS; IMMUNOGLOBULIN VARIABLE REGION; KINETICS; MICE; PROTEIN BINDING; PROTEIN ENGINEERING; RABBITS; TUMOR NECROSIS FACTOR-ALPHA; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 77950571351     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.072876     Document Type: Article

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