Pdx1-transfected adipose tissue-derived stem cells differentiate into insulin-producing cells in vivo and reduce hyperglycemia in diabetic mice

International Journal of Developmental Biology

Volumn 54, Issue 4, 2010, Pages 699-705

  Kajiyama, Hiromitsu ^a   Hamazaki, Tatsuo S ^b   Tokuhara, Makoto ^b   Masui, Shinji ^b   Okabayashi, Koji ^a   Ohnuma, Kiyoshi ^a   Yabe, Shigeharu ^b   Yasuda, Kazuki ^b   Ishiura, Shoichi ^a   Okochi, Hitoshi ^b   Asashima, Makoto ^a,c  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATIONAL CENTER FOR GLOBAL HEALTH AND MEDICINE   (Japan)

^c NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

ASC; In vivo differentiation; Pdx1; Stem cell therapy; Type 1 diabetes

Indexed keywords

C PEPTIDE; GLUCOSE; INSULIN; TRANSCRIPTION FACTOR PDX 1;

ADIPOSE TISSUE CELL; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL COUNT; CELL DIFFERENTIATION; CELL FUNCTION; CONTROLLED STUDY; ENGRAFTMENT; FEMALE; GENE EXPRESSION; GENETIC TRANSFECTION; GLUCOSE BLOOD LEVEL; HYPERGLYCEMIA; IN VITRO STUDY; IN VIVO STUDY; INSULIN DEPENDENT DIABETES MELLITUS; MOUSE; NONHUMAN; PANCREAS INJURY; PANCREAS ISLET BETA CELL; PHENOTYPE; PRIORITY JOURNAL; STEM CELL TRANSPLANTATION; STREPTOZOCIN DIABETES;

ADIPOSE TISSUE; ANIMALS; C-PEPTIDE; CELL DIFFERENTIATION; DIABETES MELLITUS; DIABETES MELLITUS, TYPE 1; HOMEODOMAIN PROTEINS; HUMANS; HYPERGLYCEMIA; INSULIN; INSULIN-SECRETING CELLS; MICE; PANCREAS; STEM CELL TRANSPLANTATION; STEM CELLS; STREPTOZOCIN; TRANS-ACTIVATORS;

MURINAE; MUS;

EID: 77950523907     PISSN: 02146282     EISSN: None     Source Type: Journal    
DOI: 10.1387/ijdb.092953hk     Document Type: Article

References (27)

1. AHLGREN, U., JONSSON, J. and EDLUND, H. (1996). The morphogenesis of the pancreatic mesenchyme is uncoupled from that of the pancreatic epithelium in IPF1/PDX1-deficient mice. Development 122: 1409-1416.
2. BLYSZCZUK, P., ASBRAND, C., ROZZO, A., KANIA, G., ST-ONGE, L., RUPNIK, M. and WOBUS, A.M. (2004). Embryonic stem cells differentiate into insulin-producing cells without selection of nestin-expressing cells. Int J Dev Biol 48: 1095-1104.
3. CAO, L.Z., TANG, D.Q., HORB, M.E., LI, S.W. and YANG, L.J. (2004). High glucose is necessary for complete maturation of Pdx1-VP16-expressing hepatic cells into functional insulin-producing cells. Diabetes 53: 3168-3178.
4. D'AMOUR, K.A., BANG, A.G., ELIAZER, S., KELLY, O.G., AGULNICK, A.D., SMART, N.G., MOORMAN, M.A., KROON, E., CARPENTER, M.K. and BAETGE, E.E. (2006). Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 24: 1392-1401.
5. GEPTS, W. (1965). Pathologic anatomy of the pancreas in juvenile diabetes mellitus. Diabetes 14: 619-633.
6. HEROLD, K.C., GITELMAN, S.E., MASHARANI, U., HAGOPIAN, W., BISIKIRSKA, B., DONALDSON, D., ROTHER, K., DIAMOND, B., HARLAN, D.M. and BLUE-STONE, J.A. (2005). A single course of anti-CD3 monoclonal antibody hOKT3g1(Ala-Ala) results in improvement in C-peptide responses and clinical parameters for at least 2 years after onset of type 1 diabetes. Diabetes 54: 1763-1769.
7. HESS, D., LI, L., MARTIN, M., SAKANO, S., HILL, D., STRUTT, B., THYSSEN, S., GRAY, D.A. and BHATIA, M. (2003). Bone marrow-derived stem cells initiate pancreatic regeneration. Nat Biotechnol 21: 763-770.
8. JIANG, W., SHI, Y., ZHAO, D., CHEN, S., YONG, J., ZHANG, J., QING, T., SUN, X., ZHANG, P., DING, M. et al. (2007). In vitro derivation of functional insulin-producing cells from human embryonic stem cells. Cell Res 17: 333-344.
9. KEYMEULEN, B., VANDEMEULEBROUCKE, E., ZIEGLER, A.G., MATHIEU, C., KAUFMAN, L., HALE, G., GORUS, F., GOLDMAN, M., WALTER, M., CANDON, S. et al. (2005). Insulin needs after CD3-antibody therapy in new-onset type 1 diabetes. N Engl J Med 352: 2598-2608.
10. KONSTANTINOVA, I., NIKOLOVA, G., OHARA-IMAIZUMI, M., MEDA, P., KUCERA, T., ZARBALIS, K., WURST, W., NAGAMATSU, S. and LAMMERT, E. (2007). EphA-Ephrin-A-mediated b cell communication regulates insulin secretion from pancreatic islets. Cell 129: 359-370.
11. KOYA, V., LU, S., SUN, Y.P., PURICH, D.L., ATKINSON, M.A., LI, S.W. and YANG, L.J. (2008). Reversal of streptozotocin-induced diabetes in mice by cellular transduction with recombinant pancreatic transcription factor pancreatic duodenal homeobox-1: a novel protein transduction domain-based therapy. Diabetes 57: 757-769.
12. LEONARD, J., PEERS, B., JOHNSON, T., FERRERI, K., LEE, S. and MONTMINY, M.R. (1993). Characterization of somatostatin transactivating factor-1, a novel homeobox factor that stimulates somatostatin expression in pancreatic islet cells. Mol Endocrinol 7: 1275-1283.
13. LI, Y., ZHANG, R., QIAO, H., ZHANG, H., WANG, Y., YUAN, H., LIU, Q., LIU, D., CHEN, L. and PEI, X. (2007). Generation of insulin-producing cells from PDX-1 gene-modified human mesenchymal stem cells. J Cell Physiol 211: 36-44.
14. LUMELSKY, N., BLONDEL, O., LAENG, P., VELASCO, I., RAVIN, R. and MCKAY, R. (2001). Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets. Science 292: 1389-1394.
15. MARCHAND, M., SCHROEDER, I.S., MARKOSSIAN, S., SKOUDY, A., N GRE, D., COSSET, F.L., REAL, P., KAISER, C., WOBUS, A.M. and SAVATIER, P. (2009). Mouse ES cells over-expressing the transcription factor NeuroD1 show increased differentiation towards endocrine lineages and insulin-expressing cells. Int J Dev Biol 53: 569-578.
16. MFOPOU, J.K., WILLEMS, E., LEYNS, L. and BOUWENS, L. (2005). Expression of regulatory genes for pancreas development during murine embryonic stem cell differentiation. Int J Dev Biol 49: 915-922.
17. MIYAZAKI, S., YAMATO, E. and MIYAZAKI, J. (2004). Regulated expression of pdx-1 promotes in vitro differentiation of insulin-producing cells from embryonic stem cells. Diabetes 53: 1030-1037.
18. NAKANISHI, M., HAMAZAKI, T.S., KOMAZAKI, S., OKOCHI, H. and ASASHIMA, M. (2007). Pancreatic tissue formation from murine embryonic stem cells in vitro. Differentiation 75: 1-11.
19. NOTKINS, A.L. and LERNMARK, A. (2001). Autoimmune type 1 diabetes: resolved and unresolved issues. J Clin Invest 108: 1247-1252.
20. OHLSSON, H., KARLSSON, K. and EDLUND, T. (1993). IPF1, a homeodomain-containing transactivator of the insulin gene. EMBO J 12: 4251-4259.
21. OKITA, K., NAKAGAWA, M., HYENJONG, H., ICHISAKA, T. and YAMANAKA, S. (2008). Generation of mouse induced pluripotent stem cells without viral vectors. Science 322: 949-953.
22. SANTANA, A., ENSENAT-WASER, R., ARRIBAS, M.I., REIG, J.A. and ROCHE, E. (2006). Insulin-producing cells derived from stem cells: recent progress and future directions. J Cell Mol Med 10: 866-883.
23. SORIA, B. (2001). In-vitro differentiation of pancreatic b-cells. Differentiation 68: 205-219.
24. TIMPER, K., SEBOEK, D., EBERHARDT, M., LINSCHEID, P., CHRIST-CRAIN, M., KELLER, U., MULLER, B. and ZULEWSKI, H. (2006). Human adipose tissue-derived mesenchymal stem cells differentiate into insulin, somatostatin, and glucagon expressing cells. Biochem Biophys Res Commun 341: 1135-1140.
25. VOLTARELLI, J.C., COURI, C.E., STRACIERI, A.B., OLIVEIRA, M.C., MORAES, D.A., PIERONI, F., COUTINHO, M., MALMEGRIM, K.C., FOSS-FREITAS, M.C., SIMOES, B.P. et al. (2007). Autologous nonmyeloablative hematopoietic stem cell transplantation in newly diagnosed type 1 diabetes mellitus. JAMA 297: 1568-1576.
26. ZHOU, Q., BROWN, J., KANAREK, A., RAJAGOPAL, J. and MELTON, D.A. (2008). In vivo reprogramming of adult pancreatic exocrine cells to b-cells. Nature 455: 627-632.
27. ZUK, P.A., ZHU, M., ASHJIAN, P., DE UGARTE, D.A., HUANG, J.I., MIZUNO, H., ALFONSO, Z.C., FRASER, J.K., BENHAIM, P. and HEDRICK, M.H. (2002). Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13: 4279-4295.