Membrane topological analysis of the proton-coupled folate transporter (PCFT-SLC46A1) by the substituted cysteine accessibility method

Biochemistry

Volumn 49, Issue 13, 2010, Pages 2925-2931

  Zhao, Rongbao ^a   Unal, Ersin Selcuk ^b   Shin, Daniel Sanghoon ^a   David Goldman, I ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b ISTANBUL UNIVERSITY   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

AFTER-TREATMENT; BIOTINYLATIONS; CYSTEINE RESIDUES; DISULFIDE BRIDGE; EXTRACELLULAR; FOLATE TRANSPORTER; FOURTH TRANSMEMBRANE DOMAINS; HELA CELL; INTRACELLULAR LOOP; MERCAPTOETHANOL; METHANETHIOSULFONATE; MOLECULAR BASIS; PERMEABILIZATION; RECESSIVE DISORDER; SECONDARY STRUCTURES; STREPTAVIDIN BEADS; TOPOLOGICAL ANALYSIS; TRANS-MEMBRANE DOMAINS; WESTERN BLOTTING;

ACTIVATION ANALYSIS; AMINO ACIDS; CYTOLOGY; ENZYME ACTIVITY; PHOSPHATASES; PROTONS;

BIOLOGICAL MEMBRANES;

CYSTEINE; DISULFIDE; MEMBRANE PROTEIN; MERCAPTOETHANOL; PROTON COUPLED FOLATE TRANSPORTER; SERINE; STREPTAVIDIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BIOTINYLATION; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; GENE MUTATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MEMBRANE STRUCTURE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN SECONDARY STRUCTURE; SUSBSTITUTED CYSTEINE ACCESSIBILITY METHOD; WESTERN BLOTTING;

BIOTINYLATION; CYSTEINE; DISULFIDES; HELA CELLS; HUMANS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR PROBE TECHNIQUES; PEPTIDE MAPPING; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY;

EID: 77950400117     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi9021439     Document Type: Article

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