Analysis of membrane topology of the human reduced folate carrier protein by hemagglutinin epitope insertion and scanning glycosylation insertion mutagenesis

Biochimica et Biophysica Acta - Biomembranes

Volumn 1564, Issue 2, 2002, Pages 333-342

  Liu, Xiang Y ^a   Matherly, Larry H ^a  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Epitope insertion; Methotrexate; Reduced folate carrier; Site directed mutagenesis; Topology

Indexed keywords

ANTIBODY; CARRIER PROTEIN; COMPLEMENTARY DNA; EPITOPE; FOLATE CARRIER PROTEIN; HEMAGGLUTININ; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; GLYCOSYLATION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; MEMBRANE STRUCTURE; MOLECULAR CLONING; MUTAGENESIS; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; WESTERN BLOTTING;

BINDING SITES; CARRIER PROTEINS; CELLS, CULTURED; EPITOPES; FLUORESCENT ANTIBODY TECHNIQUE; GLYCOSYLATION; HEMAGGLUTININS; HUMANS; MEMBRANE TRANSPORT PROTEINS; MUTAGENESIS, INSERTIONAL; PROTEIN STRUCTURE, TERTIARY;

INSERTION SEQUENCES;

EID: 0037206139     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0005-2736(02)00467-4     Document Type: Article

References (46)

1. Carrier-mediated membrane transport of folates in mammalian cells
2. Molecular and cellular biology of the human reduced folate carrier
3. A novel cDNA restores reduced folate carrier activity and methotrexate sensitivity to transport deficient cells
4. Isolation of a hamster cDNA clone coding for a function involved in methotrexate uptake
5. Isolation of a human cDNA that complements a mutant hamster cell defective in methotrexate uptake
6. Isolation of human cDNAs that restore methotrexate sensitivity and reduced folate carrier activity in methotrexate transport-defective Chinese hamster ovary cells
7. Isolation of a gene encoding a human reduced folate carrier (RFC1) and analysis of its expression in transport-deficient, methotrexate-resistant human breast cancer cells
8. Molecular cloning of the human placental folate transporter
9. Pattern of mutations that results in loss of reduced folate carrier function under antifolate selective pressure augmented by chemical mutagenesis
10. Role of the amino acid 45 residue in reduced folate carrier function and ion-dependent transport as characterized by site-directed mutagenesis
11. Mutational analysis of the functional role of conserved arginine and lysine residues in transmembrane domains of the murine reduced folate carrier
12. Functional interactions between arginine-133 and aspartate-88 in the human reduced folate carrier: Evidence for a charge-pair association
13. A mutated murine reduced folate carrier (RFC1) with increased affinity for folic acid, decreased affinity for methotrexate, and an obligatory anion requirement for transport function
14. Impaired membrane transport in methotrexate-resistant CCRF-CEM cells involves early translation termination and increased turnover of a mutant reduced folate carrier
15. A structurally altered human reduced folate carrier with increased folic acid transport mediates a novel mechanism of antifolate resistance
16. A reduced folate carrier mutation produces substrate-dependent alterations in carrier mobility in murine leukemia cells and methotrexate resistance with conservation of growth in 5-formyltetrahydrofolate
17. Mutations in the reduced folate carrier gene which confer dominant resistance to 5,10-dideazatetrahydrofolate
18. Characterization of a mutation in the reduced folate carrier in a transport defective L1210 murine leukemia cell line
19. Effects of the loss of capacity for N-glycosylation on the transport activity and cellular localization of the human reduced folate carrier
20. Topological and functional analysis of the human reduced folate carrier by hemagglutinin epitope insertion
21. Rapid formation of poly-gamma-glutamyl derivatives of methotrexate and their association with dihydrofolate reductase as assessed by high pressure liquid chromatography in the Ehrlich ascites tumor cell in vitro
22. Human K562 transfectants expressing high levels of reduced folate carrier but exhibiting low transport activity
23. Gene splicing by overlap extension: Tailor-made genes using the polymerase chain reaction
24. Identification of a highly glycosylated methotrexate membrane carrier in K562 human erythroleukemia cells up-regulated for tetrahydrofolate cofactor and methotrexate transport
25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
26. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
27. Protein measurement with the Folin phenol reagent
28. TMbase - A database of membrane spanning proteins segments
29. Characterization of transport-mediated methotrexate resistance in human tumor cells with antibodies to the membrane carrier for methotrexate and tetrahydrofolate
30. Mutations in the reduced-folate carrier affect protein localization and stability
31. Mapping the ends of transmembrane segments in a polytopic membrane protein. Scanning N-glycosylation mutagenesis of extracytosolic loops in the anion exchanger, Band 3
32. Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane
33. Analysis of the transmembrane topology of the glycine transporter GLYT1
34. Transmembrane topology of human glucose 6-phosphate transporter
35. Topology mapping of the amino-terminal half of multidrug resistance-associated protein by epitope insertion and immunofluorescence
36. Localization of cytoplasmic and extracellular domains of Na,K-ATPase by epitope tag insertion
37. Macroscopic models for studies of electrostatic interactions in proteins: Limitations and applicability
38. Salt bridge interactions: Stability of the ionic and neutral complexes in the gas phase, in solution, and in proteins
39. Charge pair interactions in a model transmembrane helix in the ER membrane
40. Role of the charge pair aspartic acid-237-lysine-358 in the lactose permease of Escherichia coli
41. Evidence for a salt bridge between transmembrane segments 5 and 6 of the yeast plasma-membrane H+-ATPase
42. Charged residues in transmembrane domains II and XI of a vesicular monoamine transporter form a charge pair that promotes high affinity substrate recognition
43. Membrane topology of Alzheimer's disease-related Presnilin I. Evidence for the existence of a molecular species with a seven membrane-spanning and one-membrane embedded structure
44. A conserved serine-rich stretch in the glutamate transporter family forms a substrate sensitive reentrant loop
45. Proximity of two oppositely oriented reentrant loops in the glutamate transporter GLT-1 identified by paired cysteine mutagenesis
46. Cys-scanning mutagenesis: A novel approach to structure-function relationships in polytopic membrane proteins