Fluorescent imaging of transition metal homeostasis using genetically encoded sensors

Current Opinion in Chemical Biology

Volumn 14, Issue 2, 2010, Pages 231-237

  Vinkenborg, Jan L ^a   Koay, Melissa S ^a   Merkx, M ^a  

^a EINDHOVEN UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA; TRANSITION ELEMENT;

BINDING AFFINITY; BIOSENSOR; CALCIUM SIGNALING; CELL INVASION; CELL ORGANELLE; CELLULAR DISTRIBUTION; CYTOSOL; DYNAMICS; FLUORESCENCE IMAGING; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC CODE; HOMEOSTASIS; NONHUMAN; PROTEIN CONFORMATION; PROTEIN DOMAIN; QUANTITATIVE ANALYSIS; REVIEW; SENSOR;

ANIMALS; BIOSENSING TECHNIQUES; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; TRANSITION ELEMENTS; ZINC;

EID: 77949900419     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpa.2009.11.022     Document Type: Review

References (51)

1. Bush A.I. The metallobiology of Alzheimer's disease. Trends Neurosci 26 (2003) 207-214
2. Strausak D., Mercer J.F., Dieter H.H., Stremmel W., and Multhaup G. Copper in disorders with neurological symptoms: Alzheimer's, Menkes, and Wilson diseases. Brain Res Bull 55 (2001) 175-185
3. Rolfs A., and Hediger M.A. Metal ion transporters in mammals: structure, function and pathological implications. J Physiol 518 (1999) 1-12
4. Finney L.A., and O'Halloran T.V. Transition metal speciation in the cell: insights from the chemistry of metal ion receptors. Science 300 (2003) 931-936
5. Nelson N. Metal ion transporters and homeostasis. EMBO J 18 (1999) 4361-4371
6. Kozlowski H., Janicka-Klos A., Brasun J., Gaggelli E., Valensin D., and Valensin G. Copper, iron, and zinc ions homeostasis and their role in neurodegenerative disorders (metal uptake, transport, distribution and regulation). Coord Chem Rev 253 (2009) 2665-2685
7. Maret W., Jacob C., Vallee B.L., and Fischer E.H. Inhibitory sites in enzymes: zinc removal and reactivation by thionein. Proc Natl Acad Sci U S A 96 (1999) 1936-1940
8. Maret W., and Li Y. Coordination dynamics of zinc in proteins. Chem Rev 109 (2009) 4682-4707
9. Luk E., Jensen L.T., and Culotta V.C. The many highways for intracellular trafficking of metals. J Biol Inorg Chem 8 (2003) 803-809
10. Theil E.C., and Goss D.J. Living with iron (and oxygen): questions and answers about iron homeostasis. Chem Rev 109 (2009) 4568-4579
11. Krezel A., and Maret W. Zinc-buffering capacity of a eukaryotic cell at physiological pZn. J Biol Inorg Chem 11 (2006) 1049-1062
12. Sekler I., Sensi S.L., Hershfinkel M., and Silverman W.F. Mechanism and regulation of cellular zinc transport. Mol Med 13 (2007) 337-343
13. Williams R.J.P., and Fraústo da Silva J.J.R. The distribution of elements in cells. Coord Chem Rev 200-202 (2000) 247-348
14. Rae T.D., Schmidt P.J., Pufahl R.A., Culotta V.C., and O'Halloran T.V. Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase. Science 284 (1999) 805-808
15. Franklin R.B., and Costello L.C. The important role of the apoptotic effects of zinc in the development of cancers. J Cell Biochem 106 (2009) 750-757
16. Mocchegiani E., Giacconi R., and Malavolta M. Zinc signalling and subcellular distribution: emerging targets in type 2 diabetes. Trends Mol Med 14 (2008) 419-428
17. Zeng L., Miller E.W., Pralle A., Isacoff E.Y., and Chang C.J. A selective turn-on fluorescent sensor for imaging copper in living cells. J Am Chem Soc 128 (2006) 10-11
18. Yang L., McRae R., Henary M.M., Patel R., Lai B., Vogt S., and Fahrni C.J. Imaging of the intracellular topography of copper with a fluorescent sensor and by synchrotron x-ray fluorescence microscopy. Proc Natl Acad Sci U S A 102 (2005) 11179-11184
19. +, and their ability to image these metals in neurobiology.
20. Nolan E.M., and Lippard S.J. Small-molecule fluorescent sensors for investigating zinc metalloneurochemistry. Acc Chem Res 42 (2009) 193-203
21. Que E.L., Domaille D.W., and Chang C.J. Metals in neurobiology: probing their chemistry and biology with molecular imaging. Chem Rev 108 (2008) 1517-1549
22. Mank M., and Griesbeck O. Genetically encoded calcium indicators. Chem Rev 108 (2008) 1550-1564
23. 2+ based on green fluorescent proteins and calmodulin. Nature 388 (1997) 882-887
24. Williams D.A., Fogarty K.E., Tsien R.Y., and Fay F.S. Calcium gradients in single smooth muscle cells revealed by the digital imaging microscope using Fura-2. Nature 318 (1985) 558-561
25. 2+ biosensor. J Am Chem Soc 124 (2002) 3522
26. Mizuno T., Murao K., Tanabe Y., Oda M., and Tanaka T. Metal-ion-dependent GFP emission in vivo by combining a circularly permutated green fluorescent protein with an engineered metal-ion-binding coiled-coil. J Am Chem Soc 129 (2007) 11378-11383
27. Jensen K.K., Martini L., and Schwartz T.W. Enhanced fluorescence resonance energy transfer between spectral variants of green fluorescent protein through zinc-site engineering. Biochemistry 40 (2001) 938-945
28. Evers T.H., Appelhof M.A.M., de Graaf-Heuvelmans P.T.H.M., Meijer E.W., and Merkx M. Ratiometric detection of Zn(II) using chelating fluorescent protein chimeras. J Mol Biol 374 (2007) 411-425
29. Evers T.H., Appelhof M.A., Meijer E.W., and Merkx M. His-tags as Zn(II) binding motifs in a protein-based fluorescent sensor. Protein Eng Des Sel 21 (2008) 529-536
30. Pearce L.L., Gandley R.E., Han W., Wasserloos K., Stitt M., Kanai A.J., McLaughlin M.K., Pitt B.R., and Levitan E.S. Role of metallothionein in nitric oxide signaling as revealed by a green fluorescent fusion protein. Proc Natl Acad Sci U S A 97 (2000) 477-482
31. Bozym R.A., Thompson R.B., Stoddard A.K., and Fierke C.A. Measuring picomolar intracellular exchangeable zinc in PC-12 cells using a ratiometric fluorescence biosensor. ACS Chem Biol 1 (2006) 103-111
32. Bozym R., Hurst T.K., Westerberg N., Stoddard A., Fierke C.A., Frederickson C.J., Thompson R.B., Ludwig B., and Michael L.J. Chapter 14: determination of zinc using carbonic anhydrase-based fluorescence biosensors. Methods Enzymol vol. 450 (2008), Academic Press 287-309
33. 2+ changes in a suspension of S. cerevisiae.
34. 2+ sensor to a specific organelle. Using zinc fingers as metal binding domains in a FRET-based sensor, the authors show changes in mitochondrial zinc levels upon stimulation with glutamate.
35. Dongen E.M.W.M.v., Dekkers L.M., Spijker K., Meijer E.W., Klomp L.W.J., and Merkx M. Ratiometric fluorescent sensor proteins with subnanomolar affinity for Zn(II) based on copper chaperone domains. J Am Chem Soc 128 (2006) 10754-10762
36. Dongen E.M.W.M.v., Evers T.H., Dekkers L.M., Meijer E.W., Klomp L.W.J., and Merkx M. Variation of linker length in ratiometric fluorescent sensor proteins allows rational tuning of Zn(II) affinity in the picomolar to femtomolar range. J Am Chem Soc 129 (2007) 3494-3495
37. 2+ concentrations that are several orders of magnitude lower than the μM sensor concentration.
38. Chimienti F., Favier A., and Seve M. ZnT-8, a pancreatic beta-cell specific zinc transporter. Biometals 18 (2005) 313-317
39. Sladek R., Rocheleau G., Rung J., Dina C., Shen L., Serre D., Boutin P., Vincent D., Belisle A., Hadjadj S., et al. A genome-wide association study identifies novel risk loci for type 2 diabetes. Nature 445 (2007) 881-885
40. Krezel A., and Maret W. Thionein/metallothionein control Zn(II) availability and the activity of enzymes. J Biol Inorg Chem 13 (2008) 401-409
41. + channels. Neuron 41 (2004) 351-365
42. Byron K.Y.B., and Miles G.C. Zinc: the brain's dark horse. Synapse 63 (2009) 1029-1049
43. Paoletti P., Vergnano A.M., Barbour B., and Casado M. Zinc at glutamatergic synapses. Neuroscience 158 (2009) 126-136
44. Bloc A., Cens T., Cruz H., and Dunant Y. Zinc-induced changes in ionic currents of clonal rat pancreatic-cells: activation of ATP-sensitive K+ channels. J Physiol 529 Pt 3 (2000) 723-734
45. Li J.Y., Ram G., Gast K., Chen X., Barasch K., Mori K., Schmidt-Ott K., Wang J., Kuo H.C., Savage-Dunn C., et al. Detection of intracellular iron by its regulatory effect. Am J Physiol Cell Physiol 287 (2004) C1547-1559
46. van den Berghe P.V., Folmer D.E., Malingre H.E., van Beurden E., Klomp A.E., van de Sluis B., Merkx M., Berger R., and Klomp L.W. Human copper transporter 2 is localized in late endosomes and lysosomes and facilitates cellular copper uptake. Biochem J 407 (2007) 49-59
47. Chen P., and He C. A general strategy to convert the MerR family proteins into highly sensitive and selective fluorescent biosensors for metal ions. J Am Chem Soc 126 (2004) 728-729
48. Piljic A., and Schultz C. Simultaneous recording of multiple cellular events by FRET. ACS Chem Biol 3 (2008) 156-160
49. Ai H.W., Hazelwood K.L., Davidson M.W., and Campbell R.E. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nat Methods 5 (2008) 401-403
50. Carlson H.J., and Campbell R.E. Genetically encoded FRET-based biosensors for multiparameter fluorescence imaging. Curr Opin Biotechnol 20 (2009) 19-27
51. 2+ fluorescent sensors, the difficulty to control their subcellular localization and concentration.