Overcoming the barriers to umbilical cord blood transplantation

Cytotherapy

Volumn 12, Issue 2, 2010, Pages 121-130

  Staba Kelly, Susan ^a   Parmar, Simrit ^a   De Lima, Marcos ^a   Robinson, Simon ^a   Shpall, Elizabeth ^a  

^a UNIVERSITY OF TEXAS   (United States)

Author keywords

Adolescent; Adult; Double unit cord blood transplant; Ex vivo expansion; Hematopoietic stem cells; Umbilical cord blood

Indexed keywords

BUSULFAN; CYCLOPHOSPHAMIDE; FLUDARABINE; HLA A1 ANTIGEN;

BLOOD TRANSFUSION; CD34 SELECTION; CELL CULTURE; CELL THERAPY; DISEASE FREE SURVIVAL; EVENT FREE SURVIVAL; EX VIVO STUDY; GRAFT VERSUS HOST REACTION; HEMATOPOIETIC STEM CELL; HLA SYSTEM; HUMAN; NEUTROPHIL; NONHUMAN; OUTCOME ASSESSMENT; PRIORITY JOURNAL; REVIEW; SAFETY; STEM CELL; THERAPY EFFECT; UMBILICAL CORD BLOOD; UMBILICAL CORD BLOOD TRANSPLANTATION;

EID: 77749289208     PISSN: 14653249     EISSN: 14772566     Source Type: Journal    
DOI: 10.3109/14653240903440111     Document Type: Review

References (103)

1. Broxmeyer HE, Hangoc G, Cooper S, Ribeiro RC, Graves V, Yoder M, et al. Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation in adults. Proc Natl Acad Sci USA. 1992;89:4109-13.
2. Cohen Y, Nagler A. Umbilical cord blood transplantation: how, when and for whom? Blood Rev. 2004;18:167-79.
3. Kurtzberg J, Laughlin M, Graham ML, Smith C, Olson JF, Halperin EC, et al. Placental blood as a source of hematopoietic stem cells for transplantation into unrelated recipients. N Engl J Med. 1996;335:157-66.
4. Migliaccio AR, Adamson JW, Stevens CE, Dobrila NL, Carrier CM, Rubinstein P. Cell dose and speed of engraftment in placental/umbilical cord blood transplantation: graft progenitor cell content is a better predictor than nucleated cell quantity. Blood. 2000;96:2717-22.
5. Gluckman E, Rocha V, Arcese W, Michel G, Sanz G, Chan KW, et al. Factors associated with outcomes of unrelated cord blood transplant: guidelines for donor choice. Exp Hematol. 2004;32:397-407.
6. Rubinstein P, Carrier C, Scaradavou A, Kurtzberg J, Adamson J, Migliaccio AR, et al. Outcomes among 562 recipients of placental-blood transplants from unrelated donors. N Engl J Med. 1998;339:1565-77.
7. Kurtzberg J, Prasad VK, Carter SL, Wagner JE, Baxter-Lowe LA, Wall D, et al. Results of the Cord Blood Transplantation Study (COBLT): clinical outcomes of unrelated donor umbilical cord blood transplantation in pediatric patients with hematologic malignancies. Blood. 2008;112:4318-27.
8. Martin PL, Carter SL, Kernan NA, Sahdev I, Wall D, Pietryga D, et al. Results of the cord blood transplantation study (COBLT): outcomes of unrelated donor umbilical cord blood transplantation in pediatric patients with lysosomal and peroxisomal storage diseases. Biol Blood Marrow Transplant. 2006;12:184-94.
9. Sawczyn KK, Quinones R, Malcolm J, Foreman N, Garrington T, Gore L, et al. Cord blood transplant in childhood ALL. Pediatr Blood Cancer. 2005;45:964-70.
10. Szabolcs P, Niedzwiecki D. Immune reconstitution after unrelated cord blood transplantation. Cytotherapy. 2007; 9:111-22.
11. Thomson BG, Robertson KA, Gowan D, Heilman D, Broxmeyer HE, Emanuel D, et al. Analysis of engraftment, graft-versus-host disease, and immune recovery following unrelated donor cord blood transplantation. Blood. 2000;96:2703-11.
12. Weinreb S, Delgado JC, Clavijo OP, Yunis EJ, Bayer-Zwirello L, Polansky L, et al. Transplantation of unrelated cord blood cells. Bone Marrow Transplant. 1998;22: 193-6.
13. Barker JN, Weisdorf DJ, DeFor TE, Blazar BR, Miller JS, Wagner JE. Rapid and complete donor chimerism in adult recipients of unrelated donor umbilical cord blood transplantation after reduced-intensity conditioning. Blood. 2003;102:1915-9.
14. De Lima M, St John LS, Wieder ED, Lee MS, McMannis J, Karandish S, et al. Double-chimaerism after transplantation of two human leucocyte antigen mismatched, unrelated cord blood units. Br J Haematol. 2002;119:773-6.
15. Fernandez MN, Regidor C, Cabrera R, Garcia-Marco J, Briz M, Fores R, et al. Cord blood transplants: early recovery of neutrophils from co-transplanted sibling haploidentical progenitor cells and lack of engraftment of cultured cord blood cells, as ascertained by analysis of DNA polymorphisms. Bone Marrow Transplant. 2001;28:355-63.
16. Shpall EJ, Quinones R, Giller R, Zeng C, Baron AE, Jones RB, et al. Transplantation of ex vivo expanded cord blood. Biol Blood Marrow Transplant. 2002;8:368-76.
17. Pecora AL, Stiff P, Jennis A, Goldberg S, Rosenbluth R, Price P, et al. Prompt and durable engraftment in two older adult patients with high risk chronic myelogenous leukemia (CML) using ex vivo expanded and unmanipulated unrelated umbilical cord blood. Bone Marrow Transplant. 2000;25:797-9.
18. Jaroscak J, Goltry K, Smith A, Waters-Pick B, Martin PL, Driscoll TA, et al. Augmentation of umbilical cord blood (UCB) transplantation with ex vivo-expanded UCB cells: results of a phase 1 trial using the Aastrom Replicell System. Blood. 2003;101:5061-7.
19. Pecora AL, Stiff P, LeMaistre CF, Bayer R, Bachier C, Goldberg SL, et al. A phase II trial evaluating the safety and effectiveness of the AastromReplicell system for augmentation of low-dose blood stem cell transplantation. Bone Marrow Transplant. 2001;28:295-303.
20. de Lima M, McMannis J, Gee A, Komanduri K, Couriel D, Andersson BS, et al. Transplantation of ex vivo expanded cord blood cells using the copper chelator tetraethylenepentamine: a phase I/II clinical trial. Bone Marrow Transplant. 2008;41:771-8.
21. Delaney C, Brashem-Stein C, Voorhies H, Gutman J, Dallas M, Heimfeld S et al. Notch-Mediated Expansion of Human Cord Blood Progenitor Cells Results in Rapid Myeloid Reconstitution in Vivo Following Myeloablative Cord Blood Transplantaion Blood. Blood (ASH Annual Meeting Abstracts) 2008; 112: Abstract 212.
22. de Lima M, McNiece I, McMannis J, Hosing C, Kebraei P, Komanduri K, et al. Double Cord Blood Transplantations (CBT) with Ex-vivo expansion (EXP) of one unit utilizing a mesenchymal stromal cell (MSC) Platform. Biology of Blood and Marrow Transplantation 2009;15: No. 2, Supplement, Abstract 122.
23. Jaing TH, Yang CP, Hung IJ, Chen SH, Sun CF, Chow R. Transplantation of unrelated donor umbilical cord blood utilizing double-unit grafts for five teenagers with transfusion-dependent thalassemia. Bone Marrow Transplant. 2007;40:307-11.
24. Fernandes J, Rocha V, Robin M, de Latour RP, Traineau R, Devergie A, et al. Second transplant with two unrelated cord blood units for early graft failure after haemato poietic stem cell transplantation. Br J Haematol. 2007;137:248-51.
25. Wang FR, Huang XJ, Zhang YC, Chen YH, Lu DP. Successful transplantation of double unit umbilical-cord blood from unrelated donors in high risk leukemia with a long follow-up. Chin Med J (Engl). 2005;118:772-6.
26. Tarnani M, Laurenti L, Chiusolo P, Sora F, Innocenti I, Leone G, et al. Simultaneous double mismatched cord blood transplantation in a young patient with secondary myelodys-plastic syndrome: feasibility and complications. Leuk Lymphoma. 2008;49:821-3.
27. Sarkodee-Adoo C, Alvarnas J, Briggs A, Schriber J, Karanes C, Nademanee A, et al. Emergency double unrelated umbilical cord blood transplant for acute lymphoblastic leukemia after very late deferral of bone marrow donor. Bone Marrow Transplant. 2008;41:675-6.
28. Yen HJ, Chiou TJ, Hung GY, Chang CY, Hsieh MY, Tzeng CH, et al. Long-term mixed full-donor chimerism with dominance reversion after a double-unit cord blood transplant. Eur J Haematol. 2008;80:366-7.
29. Majhail NS, Weisdorf DJ, Wagner JE, Defor TE, Brunstein CG, Burns LJ. Comparable results of umbilical cord blood and HLA-matched sibling donor hematopoietic stem cell transplantation after reduced-intensity preparative regimen for advanced Hodgkin lymphoma. Blood. 2006;107:3804-7.
30. Gluckman E, Broxmeyer HA, Auerbach AD, Friedman HS, Douglas GW, Devergie A, et al. Hematopoietic reconstitution in a patient with Fanconi's anemia by means of umbilicalcord blood from an HLA-identical sibling. N Engl J Med. 1989;321:1174-8.
31. Ballen KK, Barker JN, Stewart SK, Greene MF, Lane TA. Collection and preservation of cord blood for personal use. Biol Blood Marrow Transplant. 2008;14:356-63.
32. Broxmeyer HE, Douglas GW, Hangoc G, Cooper S, Bard J, English D, et al. Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells. Proc Natl Acad Sci USA. 1989;86:3828-32.
33. Rachamim N, Gan J, Segall H, Krauthgamer R, Marcus H, Berrebi A, et al. Tolerance induction by 'megadose' hematopoietic transplants: donor-type human CD34 stem cells induce potent specific reduction of host anti-donor cytotoxic T lymphocyte precursors in mixed lymphocyte culture. Transplantation. 1998;65:1386-93.
34. Barker JN, Weisdorf DJ, Wagner JE. Creation of a double chimera after the transplantation of umbilical-cord blood from two partially matched unrelated donors. N Engl J Med. 2001;344:1870-1.
35. JN Barker, DJ Weisdorf, TE DeFor, CG Brunstein, and JE Wagner. Umbilical cord blood (UCB) transplantation after non-myeloablative (NMA) conditioning for advanced follicular lymphoma, mantle cell lymphoma and chronic lymphocytic leukemia: low transplant-related mortality and high progression-free survival. Blood. 2005;106.
36. Barker JN, Weisdorf DJ, DeFor TE, Blazar BR, McGlave PB, Miller JS, et al. Transplantation of 2 partially HLA-matched umbilical cord blood units to enhance engraftment in adults with hematologic malignancy. Blood. 2005;105:1343-7.
37. Ballen KK, Spitzer TR, Yeap BY, McAfee S, Dey BR, Attar E, et al. Double unrelated reduced-intensity umbilical cord blood transplantation in adults. Biol Blood Marrow Transplant. 2007;13:82-9.
38. Delaney M, Cutler CS, Haspel RL, Yeap BY, McAfee SL, Dey BR, et al. High-resolution HLA matching in double-umbilical cord blood reduced-intensity transplantation in adults. Transfusion. 2009;49:995-1002.
39. Haspel RL, Kao G, Yeap BY, Cutler C, Soiffer RJ, Alyea EP, et al. Preinfusion variables predict the predominant unit in the setting of reduced-intensity double cord blood transplantation. Bone Marrow Transplant. 2008;41:523-9.
40. Lister J, Gryn JF, McQueen KL, Harris DT, Rossetti JM, Shadduck RK. Multiple unit HLA-unmatched sex-mismatched umbilical cord blood transplantation for advanced hematological malignancy. Stem Cells Dev. 2007;16:177-86.
41. JN Barker, A Scaradavou, C Stevens, P Rubinstein. The dose-match interaction in umbilical cord blood (UCB) transplantation: an analysis of the impact of cell dose and HLA-match on the disease free survival (DFS) of 989 patients transplanted with single units for hematological malignancy. Blood. 2007;110:144a.
42. MR Verneris, C Brunstein, Te DeFor, J Barker, DJ Weisdorf, BR Blazar, JS Miller, and JE Wagner, et al. Risk of relapse (REL) after umbilical cord blood transplantation (UCBT) in patients with acute leukemia: marked reduction in recipients of two units. Blood. 2005;106:93A.
43. Bradstock K, Hertzberg M, Kerridge I, Svennilson J, George B, McGurgan M, et al. Single versus double unrelated umbilical cord blood units for allogeneic transplantation in adults with advanced hematological malignancies: a retrospective comparison of outcomes. Intern Med J. 2009 Nov;39(11):744-51.
44. CG Brunstein, JN Barker, TE DeFor, K French, DJ Weisdorf, and JE Wagner et al. Non-myeloablative (NMA) umbilical cord blood transplantation (UCBT): promising disease-free survival in 95 consecutive patients. Blood. 2005;106.
45. Williams DA. Ex vivo expansion of hematopoietic stem and progenitor cells: robbing Peter to pay Paul? Blood. 1993; 81:3169-72.
46. McNiece IK, Almeida-Porada G, Shpall EJ, Zanjani E. Ex vivo expanded cord blood cells provide rapid engraftment in fetal sheep but lack long-term engrafting potential. Exp Hematol. 2002;30:612-6.
47. Holyoake TL, Alcorn MJ, Richmond L, Farrell E, Pearson C, Green R, et al. CD34 positive PBPC expanded ex vivo may not provide durable engraftment following myeloablative chemoradiotherapy regimens. Bone Marrow Transplant. 1997;19:1095-101.
48. Purdy MH, Hogan CJ, Hami L, McNiece I, Franklin W, Jones RB, et al. Large volume ex vivo expansion of CD34-positive hematopoietic progenitor cells for transplantation. J Hematother. 1995;4:515-25.
49. McNiece I, Jones R, Cagnoni P, Bearman S, Nieto Y, Shpall EJ. Ex-vivo expansion of hematopoietic progenitor cells: preliminary results in breast cancer. Hematol Cell Ther. 1999;41:82-6.
50. Von Drygalski A, Alespeiti G, Ren L, Adamson JW. Murine bone marrow cells cultured ex vivo in the presence of multiple cytokine combinations lose radioprotective and long-term engraftment potential. Stem Cells Dev. 2004;13:101-11.
51. Lazzari L, Lucchi S, Rebulla P, Porretti L, Puglisi G, Lecchi L, et al. Long-term expansion and maintenance of cord blood haematopoietic stem cells using thrombo poietin, Flt3-ligand, interleukin (IL)-6 and IL-11 in a serum-free and stroma-free culture system. Br J Haematol. 2001;112:397-404.
52. Filip S, Vavrova J, Vokurkova D, Blaha M, Vanasek J. Myeloid differentiation and maturation of SCF1IL-31IL-11 expanded AC1331/CD341 cells selected from high-risk breast cancer patients. Neoplasma. 2000;47:73-80.
53. Vavrova J, Filip S, Vokurkova D, Blaha M, Vanasek J, Jebavy L. Ex vivo expansion CD341/AC1331-selected autologous peripheral blood progenitor cells (PBPC) in high-risk breast cancer patients receiving intensive chemotherapy. Hematol Cell Ther. 1999;41:105-12.
54. Glimm H, Eaves CJ. Direct evidence for multiple self-renewal divisions of human in vivo repopulating hematopoietic cells in short-term culture. Blood. 1999;94:2161-8.
55. Glimm H, Oh IH, Eaves CJ. Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G/M transit and do not reenter G. Blood. 2000;96:4185-93.
56. McNiece I, Jones R, Bearman SI, Cagnoni P, Nieto Y, Franklin W, et al. Ex vivo expanded peripheral blood progenitor cells provide rapid neutrophil recovery after high-dose chemotherapy in patients with breast cancer. Blood. 2000;96:3001-7.
57. McNiece I, Kubegov D, Kerzic P, Shpall EJ, Gross S. Increased expansion and differentiation of cord blood products using a two-step expansion culture. Exp Hematol. 2000;28:1181-6.
58. de Lima M, McMannis JD, Saliba R, Worth L, Kebriaei P, Popat U et al. Double Cord Blood Transplantation (CBT) with and without Ex-Vivo Expansion (EXP): A Randomized, Controlled Study. Blood (ASH Annual Meeting Abstracts) 2008;112: Abstract 154.
59. Mohamed AA, Ibrahim AM, El-Masry MW, Mansour IM, Khroshied MA, Gouda HM, et al. Ex vivo expansion of stem cells: defining optimum conditions using various cytokines. Lab Hematol. 2006;12:86-93.
60. Piacibello W, Sanavio F, Garetto L, Severino A, Dane A, Gammaitoni L, et al. Differential growth factor requirement of primitive cord blood hematopoietic stem cell for self-renewal and amplification vs proliferation and differentiation. Leukemia. 1998;12:718-27.
61. Yao CL, Chu IM, Hsieh TB, Hwang SM. A systematic strategy to optimize ex vivo expansion medium for human hematopoietic stem cells derived from umbilical cord blood mononuclear cells. Exp Hematol. 2004;32:720-7.
62. Lazzari L, Lucchi S, Porretti L, Montemurro T, Giordano R, Lopa R, et al. Comparison of different serum-free media for ex vivo expansion of HPCs from cord blood using thrombopoietin, Flt-3 ligand, IL-6, and IL-11. Transfusion. 2001;41:718-9.
63. Yao CL, Feng YH, Lin XZ, Chu IM, Hsieh TB, Hwang SM. Characterization of serum-free ex vivo-expanded hematopoietic stem cells derived from human umbilical cord blood CD133(1) cells. Stem Cells Dev. 2006;15:70-8.
64. Peled T, Landau E, Mandel J, Glukhman E, Goudsmid NR, Nagler A, et al. Linear polyamine copper chelator tetraethylenepentamine augments long-term ex vivo expansion of cord blood-derived CD341 cells and increases their engraftment potential in NOD/SCID mice. Exp Hematol. 2004; 32:547-55.
65. Young JC, Wu S, Hansteen G, Du C, Sambucetti L, Remiszewski S, et al. Inhibitors of histone deacetylases promote hematopoietic stem cell self-renewal. Cytotherapy. 2004;6: 328-36.
66. Sato N, Meijer L, Skaltsounis L, Greengard P, Brivanlou AH. Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat Med. 2004; 10:55-63.
67. Delaney C, Varnum-Finney B, Aoyama K, Brashem-Stein C, Bernstein ID. Dose-dependent effects of the Notch ligand Delta1 on ex vivo differentiation and in vivo marrow repopulating ability of cord blood cells. Blood. 2005; 106:2693-9.
68. Schofield R. The stem cell system. Biomed Pharmacother. 1983;37:375-80.
69. Lemischka IR, Moore KA. Stem cells: interactive niches. Nature. 2003;425:778-9.
70. Fuchs E, Tumbar T, Guasch G. Socializing with the neighbors: stem cells and their niche. Cell. 2004;116:769-78.
71. Allen TD, Dexter TM. The essential cells of the hemopoietic microenvironment. Exp Hematol. 1984;12:517-21.
72. Dexter TM, Coutinho LH, Spooncer E, Heyworth CM, Daniel CP, Schiro R, et al. Stromal cells in haemopoiesis. Ciba Found Symp. 1990;148:76-95.
73. Yamazaki K, Roberts RA, Spooncer E, Dexter TM, Allen TD. Cellular interactions between 3T3 cells and interleukin-3-dependent multipotent haemopoietic cells: a model system for stromal-cell-mediated haemopoiesis. J Cell Physiol. 1989;139:301-12.
74. Gartner S, Kaplan HS. Long-term culture of human bone marrow cells. Proc Natl Acad Sci USA. 1980;77:4756-9.
75. Etheridge SL, Spencer GJ, Heath DJ, Genever PG. Expression profiling and functional analysis of wnt signaling mechanisms in mesenchymal stem cells. Stem Cells. 2004;22: 849-60.
76. Kadereit S, Deeds LS, Haynesworth SE, Koc ON, Kozik MM, Szekely E, et al. Expansion of LTC-ICs and maintenance of p21 and BCL-2 expression in cord blood CD34(1)/CD38(-) early progenitors cultured over human MSCs as a feeder layer. Stem Cells. 2002; 20:573-82.
77. Rattis FM, Voermans C, Reya T. Wnt signaling in the stem cell niche. Curr Opin Hematol. 2004;11:88-94.
78. Majumdar MK, Thiede MA, Haynesworth SE, Bruder SP, Gerson SL. Human marrow-derived mesenchymal stem cells (MSCs) express hematopoietic cytokines and support long-term hematopoiesis when differentiated toward stromal and osteogenic lineages. J Hematother Stem Cell Res. 2000;9: 841-8.
79. Zhang Y, Li C, Jiang X, Zhang S, Wu Y, Liu B, et al. Human placenta-derived mesenchymal progenitor cells support culture expansion of long-term culture-initiating cells from cord blood CD341 cells. Exp Hematol. 2004;32:657-64.
80. Yamaguchi M, Hirayama F, Murahashi H, Azuma H, Sato N, Miyazaki H, et al. Ex vivo expansion of human UC blood primitive hematopoietic progenitors and transplantable stem cells using human primary BM stromal cells and human AB serum. Cytotherapy. 2002;4:109-18.
81. Kanai M, Hirayama F, Yamaguchi M, Ohkawara J, Sato N, Fukazawa K, et al. Stromal cell-dependent ex vivo expansion of human cord blood progenitors and augmentation of transplantable stem cell activity. Bone Marrow Transplant. 2000;26:837-44.
82. in't Anker PS, Noort WA, Kruisselbrink AB, Scherjon SA, Beekhuizen W, Willemze R, et al. Nonexpanded primary lung and bone marrow-derived mesenchymal cells promote the engraftment of umbilical cord blood-derived CD34(+) cells in NOD/SCID mice. Exp Hematol. 2003;31:881-9.
83. Noort WA, Kruisselbrink AB, in't Anker PS, Kruger M, van Bezooijen RL, de Paus RA, et al. Mesenchymal stem cells promote engraftment of human umbilical cord blood-derived CD34(+) cells in NOD/SCID mice. Exp Hematol. 2002;30:870-8.
84. Ahrens N, Tormin A, Paulus M, Roosterman D, Salama A, Krenn V, et al. Mesenchymal stem cell content of human vertebral bone marrow. Transplantation. 2004;78:925-9.
85. Le Blanc K, Rasmusson I, Sundberg B, Gotherstrom C, Hassan M, Uzunel M, et al. Treatment of severe acute graft-versus-host disease with third party haploidentical mesenchymal stem cells. Lancet. 2004;363:1439-41.
86. Gotherstrom C, Ringden O, Tammik C, Zetterberg E, West-gren M, Le Blanc K. Immunologic properties of human fetal mesenchymal stem cells. Am J Obstet Gynecol. 2004;190: 239-45.
87. Rasmusson I, Ringden O, Sundberg B, Le Blanc K. Mesenchymal stem cells inhibit the formation of cytotoxic T lymphocytes, but not activated cytotoxic T lymphocytes or natural killer cells. Transplantation. 2003;76:1208-13.
88. Zhang J, Niu C, Ye L, Huang H, He X, Tong WG, et al. Identification of the haematopoietic stem cell niche and control of the niche size. Nature. 2003;425:836-41.
89. McNiece I, Harrington J, Turney J, Kellner J, Shpall EJ. Ex vivo expansion of cord blood mononuclear cells on mesenchymal stem cells. Cytotherapy. 2004;6:311-7.
90. Le Blanc K, Rasmusson I, Gotherstrom C, Seidel C, Sundberg B, Sundin M, et al. Mesenchymal stem cells inhibit the expression of CD25 (interleukin-2 receptor) and CD38 on phytohaemagglutinin-activated lymphocytes. Scand J Immunol. 2004;60:307-15.
91. De Angeli S, Di Liddo R, Buoro S, Toniolo L, Conconi MT, Belloni AS, et al. New immortalized human stromal cell lines enhancing in vitro expansion of cord blood hematopoietic stem cells. Int J Mol Med. 2004;13:363-71.
92. Emerson SG, Palsson BO, Clarke MF, Silver SM, Adams PT, Koller MR, et al. In vitro expansion of hematopoietic cells for clinical application. Cancer Treat Res. 1995;76:215-23.
93. Van Zant G, Rummel SA, Koller MR, Larson DB, Drubachevsky I, Palsson M, et al. Expansion in biore actors of human progenitor populations from cord blood and mobilized peripheral blood. Blood Cells. 1994;20: 482-91.
94. Koller MR, Manchel I, Newsom BS, Palsson MA, Palsson BO. Bioreactor expansion of human bone marrow: comparison of unprocessed, density-separated, and CD34-enriched cells. J Hematother. 1995;4:159-69.
95. Tsai S, Emerson SG, Sieff CA, Nathan DG. Isolation of a human stromal cell strain secreting hemopoietic growth factors. J Cell Physiol. 1986;127:137-45.
96. Astori G, Adami V, Mambrini G, Bigi L, Cilli M, Facchini A, et al. Evaluation of ex vivo expansion and engraftment in NOD-SCID mice of umbilical cord blood CD341 cells using the DIDECO 'Pluricell System'. Bone Marrow Transplant. 2005;35:1101-6.
97. Brunstein CG, Barker JN, Weisdorf DJ, Defor TE, McKenna D, Chong SY, et al. Intra-BM injection to enhance engraftment after myeloablative umbilical cord blood transplantation with two partially HLA-matched units. Bone Marrow Transplant. 2009;43:935-40.
98. Frassoni F, Gualandi F, Podesta M, Raiola AM, Ibatici A, Piaggio G, et al. Direct intrabone transplant of unrelated cord-blood cells in acute leukaemia: a phase I/II study. Lancet Oncol. 2008;9:831-9.
99. Dai HS, Gao JT, Zhang TW, Yang Z, Che YZ, Zheng YZ. [Study on the mechanism of enhancing homing efficiency of human hematopoietic stem/progenitor cells into bone marrow after manipulation with tumor necrosis factor alpha in xenotransplanted BALB/c mouse model]. Zhonghua Xue Ye Xue Za Zhi. 2009;30:97-102.
100. Hao M, Meng HX, Li G, Qi PJ, Xu Y, Li CH, et al. [Study of influence of umbilical cord mesenchymal stem cells on CD341 cells in vivo homing in NOD/SCID]. Zhonghua Xue Ye Xue Za Zhi. 2009;30:103-6.
101. Ohno N, Kajiume T, Sera Y, Sato T, Kobayashi M. Short-term culture of umbilical cord blood-derived CD34 cells enhances engraftment into NOD/SCID mice through increased CXCR4 expression. Stem Cells Dev. 2009;18(8):1221-6.
102. Mazur MA, Davis CC, Szabolcs P. Ex vivo expansion and Th1/Tc1 maturation of umbilical cord blood T cells by CD3/CD28 costimulation. Biol Blood Marrow Transplant. 2008;14:1190-6.
103. Boissel L, Tuncer HH, Betancur M, Wolfberg A, Klingemann H. Umbilical cord mesenchymal stem cells increase expansion of cord blood natural killer cells. Biol Blood Marrow Transplant. 2008;14:1031-8.