Heterologous expression and purification of a heat-tolerant staphylococcus xylosus lipase

Molecular Biotechnology

Volumn 44, Issue 2, 2010, Pages 110-119

  Brod, Fábio Cristiano Angonesi ^a   Pelisser, Márcia Regina ^a   Bertoldo, Jean Borges ^a   Vernal, Javier ^a   Bloch Jr , Carlos ^b   Terenzi, Hernán ^a   Arisi, Ana Carolina Maisonnave ^a  

^a FEDERAL UNIVERSITY OF SANTA CATARINA   (Brazil)

^b EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA   (Brazil)

Author keywords

E. coli expression; Lipase; Staphylococcus xylosus

Indexed keywords

E. COLI; E. COLI EXPRESSION; EXPRESSION VECTORS; EXTRACELLULAR LIPASE; FUSION PROTEINS; HETEROLOGOUS EXPRESSION; INCUBATION PERIODS; INITIAL ACTIVITY; LIPASE ACTIVITY; LIPASE GENE; MEAT PRODUCTS; P-NITROPHENYL ACETATE; THERMAL STABILITY;

AFFINITY CHROMATOGRAPHY; CHROMATOGRAPHIC ANALYSIS; CLONING; ESCHERICHIA COLI; ESTERS; GENE EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PHOSPHATASES; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY;

LIPASES;

ACETIC ACID; BUTYRIC ACID; ESTER; HISTIDINE; HYBRID PROTEIN; RECOMBINANT ENZYME; TRIACYLGLYCEROL LIPASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; EXPRESSION VECTOR; FERMENTED PRODUCT; GENE OVEREXPRESSION; HETEROLOGOUS EXPRESSION; MEAT; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; STAPHYLOCOCCUS XYLOSUS; THERMOSTABILITY;

BACTERIAL PROTEINS; CHROMATOGRAPHY, GEL; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; LIPASE; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCUS; SUBSTRATE SPECIFICITY; TEMPERATURE;

STAPHYLOCOCCUS XYLOSUS;

EID: 77649237785     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-009-9218-0     Document Type: Article

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