Isolation of a novel lipase gene from Serratia liquefaciens S33 DB-1, functional expression in Pichia pastoris and its properties

Molecular Biotechnology

Volumn 38, Issue 2, 2008, Pages 99-107

  Yao, Hongyan ^a   Yu, Shunwu ^b   Zhang, Lida ^a   Zuo, Kaijing ^a   Ling, Hua ^a   Zhang, Fei ^a   Tang, Kexuan ^a,c  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

^b INSTITUTE OF EDIBLE FUNGI   (China)

^c FUDAN UNIVERSITY   (China)

Author keywords

Cloning; Expression; Genomic walking; Lipase; Pichia pastoris; Serratia liquefaciens

Indexed keywords

ACIDS; AMINES; AMINO ACIDS; ENZYME ACTIVITY; FOOD ADDITIVES; ORGANIC ACIDS; PROTEINS;

AGAROSE; CLONING; EXPRESSION; EXPRESSION VECTORS; FUNCTIONAL EXPRESSION; GENOME SEQUENCING; GENOMIC-WALKING; HIS-TAG; LAURATE; LIPASE; LIPASE ACTIVITY; LIPASE GENE; METHYLOTROPHIC YEASTS; OPEN READING FRAME; PICHIA PASTORIS; RECOMBINANT ENZYMES; RECOMBINANT PROTEINS; SERRATIA; SERRATIA LIQUEFACIENS; TRANSFORMANTS;

GENE ENCODING;

4 NITROPHENYL CAPRATE; 4 NITROPHENYL MYRISTATE; 4 NITROPHENYL STEARATE; AGAROSE; AMINO ACID; ESTER DERIVATIVE; LAURIC ACID 4 NITROPHENYL ESTER; OXIDOREDUCTASE; PALMITIC ACID 4 NITROPHENYL ESTER; POLYHISTIDINE TAG; RECOMBINANT ENZYME; RECOMBINANT PROTEIN; TRIACYLGLYCEROL; UNCLASSIFIED DRUG; TRIACYLGLYCEROL LIPASE;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; EXPRESSION VECTOR; GENE EXPRESSION; GENE ISOLATION; GENETIC ANALYSIS; GENETIC CODE; GENETIC TRANSFORMATION; GENOME ANALYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN MOTIF; PROTEIN PURIFICATION; PROTEIN SECRETION; SEQUENCE ANALYSIS; SERRATIA LIQUEFACIENS; SLLIPA GENE; AMINO ACID SEQUENCE; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PICHIA; SEQUENCE ALIGNMENT; TRANSGENE;

PICHIA PASTORIS; SERRATIA LIQUEFACIENS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; LIPASE; MOLECULAR SEQUENCE DATA; PICHIA; SEQUENCE ALIGNMENT; SERRATIA LIQUEFACIENS; SUBSTRATE SPECIFICITY; TRANSGENES;

EID: 42449114153     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-007-9007-6     Document Type: Article

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