Deletion of Candida albicans SPT6 is not lethal but results in defective hyphal growth

Fungal Genetics and Biology

Volumn 47, Issue 4, 2010, Pages 288-296

  Al Rawi, Nada ^a   Laforce Nesbitt, Sonia S ^a   Bliss, Joseph M ^a,b  

^a WOMEN AND INFANTS HOSPITAL   (United States)

^b BROWN UNIVERSITY   (United States)

Author keywords

Candida albicans; Hyphae; Saccharomyces cerevisiae; Single chain variable fragment

Indexed keywords

COMPLEMENTARY DNA; FUNGAL PROTEIN; PROTEIN SPT6; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TRANSCRIPTION ELONGATION FACTOR; UNCLASSIFIED DRUG;

3' UNTRANSLATED REGION; ARTICLE; CANDIDA ALBICANS; CONTROLLED STUDY; DNA LIBRARY; FUNGAL STRAIN; FUNGUS GROWTH; FUNGUS HYPHAE; GENE DELETION; GENE EXPRESSION; GENE FUNCTION; HOMOZYGOTE; IMMUNOREACTIVITY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FUNCTION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CANDIDA ALBICANS; DNA, FUNGAL; FUNGAL PROTEINS; GENE DELETION; GENE EXPRESSION; GENE LIBRARY; HUMANS; HYPHAE; MOLECULAR SEQUENCE DATA; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTIONAL ELONGATION FACTORS;

CANDIDA ALBICANS; SACCHAROMYCES CEREVISIAE;

EID: 77649187535     PISSN: 10871845     EISSN: 10960937     Source Type: Journal    
DOI: 10.1016/j.fgb.2010.01.001     Document Type: Article

References (45)

1. Alloush H.M., Lopez-Ribot J.L., Masten B.J., and Chaffin W.L. 3-phosphoglycerate kinase: a glycolytic enzyme protein present in the cell wall of Candida albicans. Microbiology 143 Pt 2 (1997) 321-330
2. Andrulis E.D., Guzman E., Doring P., Werner J., and Lis J.T. High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation. Genes Dev. 14 (2000) 2635-2649
3. Angiolella L., Facchin M., Stringaro A., Maras B., Simonetti N., and Cassone A. Identification of a glucan-associated enolase as a main cell wall protein of Candida albicans and an indirect target of lipopeptide antimycotics. J. Infect. Dis. 173 (1996) 684-690
4. Arnaud M.B., Costanzo M.C., Skrzypek M.S., Shah P., Binkley G., Lane C., Miyasato S.R., and Sherlock G. Sequence resources at the Candida Genome Database. Nucleic Acids Res. 35 (2007) D452-D456
5. Benjamin Jr. D.K., Stoll B.J., Fanaroff A.A., McDonald S.A., Oh W., Higgins R.D., Duara S., Poole K., Laptook A., and Goldberg R. Neonatal candidiasis among extremely low birth weight infants: risk factors, mortality rates, and neurodevelopmental outcomes at 18-22 months. Pediatrics 117 (2006) 84-92
6. Bliss J.M., Sullivan M.A., Malone J., and Haidaris C.G. Differentiation of Candida albicans and Candida dubliniensis by using recombinant human antibody single-chain variable fragments specific for hyphae. J. Clin. Microbiol. 41 (2003) 1152-1160
7. Bortvin A., and Winston F. Evidence that Spt6p controls chromatin structure by a direct interaction with histones. Science 272 (1996) 1473-1476
8. Calderone R.A. Candida and Candidiasis (2002), ASM Press, Washington, DC
9. Care R.S., Trevethick J., Binley K.M., and Sudbery P.E. The MET3 promoter: a new tool for Candida albicans molecular genetics. Mol. Microbiol. 34 (1999) 792-798
10. Cheng S., Clancy C.J., Checkley M.A., Handfield M., Hillman J.D., Progulske-Fox A., Lewin A.S., Fidel P.L., and Nguyen M.H. Identification of Candida albicans genes induced during thrush offers insight into pathogenesis. Mol. Microbiol. 48 (2003) 1275-1288
11. Cheng S., Clancy C.J., Checkley M.A., Zhang Z., Wozniak K.L., Seshan K.R., Jia H.Y., Fidel Jr. P., Cole G., and Nguyen M.H. The role of Candida albicans NOT5 in virulence depends upon diverse host factors in vivo. Infect. Immun. 73 (2005) 7190-7197
12. Clark-Adams C.D., and Winston F. The SPT6 gene is essential for growth and is required for delta-mediated transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 7 (1987) 679-686
13. Dengl S., Mayer A., Sun M., and Cramer P. Structure and in vivo requirement of the yeast Spt6 SH2 domain. J. Mol. Biol. 389 (2009) 211-225
14. Feng Q., Summers E., Guo B., and Fink G. Ras signaling is required for serum-induced hyphal differentiation in Candida albicans. J. Bacteriol. 181 (1999) 6339-6346
15. Gillum A.M., Tsay E.Y., and Kirsch D.R. Isolation of the Candida albicans gene for orotidine-5′-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations. Mol. Gen. Genet. 198 (1984) 179-182
16. Gil-Navarro I., Gil M.L., Casanova M., O'Connor J.E., Martinez J.P., and Gozalbo D. The glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase of Candida albicans is a surface antigen. J. Bacteriol. 179 (1997) 4992-4999
17. Haidaris C.G., Malone J., Sherrill L.A., Bliss J.M., Gaspari A.A., Insel R.A., and Sullivan M.A. Recombinant human antibody single chain variable fragments reactive with Candida albicans surface antigens. J. Immunol. Methods 257 (2001) 185-202
18. Hartzog G.A., Wada T., Handa H., and Winston F. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes Dev. 12 (1998) 357-369
19. Kadosh D., and Johnson A.D. Rfg1, a protein related to the Saccharomyces cerevisiae hypoxic regulator Rox1, controls filamentous growth and virulence in Candida albicans. Mol. Cell. Biol. 21 (2001) 2496-2505
20. Kadosh D., and Johnson A.D. Induction of the Candida albicans filamentous growth program by relief of transcriptional repression: a genome-wide analysis. Mol. Biol. Cell 16 (2005) 2903-2912
21. Kaplan C.D., Laprade L., and Winston F. Transcription elongation factors repress transcription initiation from cryptic sites. Science 301 (2003) 1096-1099
22. Kaplan C.D., Morris J.R., Wu C., and Winston F. Spt5 and spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster. Genes Dev. 14 (2000) 2623-2634
23. Kumamoto C.A., and Vinces M.D. Contributions of hyphae and hypha-co-regulated genes to Candida albicans virulence. Cell Microbiol. 7 (2005) 1546-1554
24. Laforce-Nesbitt S.S., Sullivan M.A., Hoyer L.L., and Bliss J.M. Inhibition of Candida albicans adhesion by recombinant human antibody single-chain variable fragment specific for Als3p. FEMS Immunol. Med. Microbiol. (2008)
25. Leberer E., Harcus D., Broadbent I.D., Clark K.L., Dignard D., Ziegelbauer K., Schmidt A., Gow N.A., Brown A.J., and Thomas D.Y. Signal transduction through homologs of the Ste20p and Ste7p protein kinases can trigger hyphal formation in the pathogenic fungus Candida albicans. Proc. Natl. Acad. Sci. USA 93 (1996) 13217-13222
26. Liu H., Kohler J., and Fink G.R. Suppression of hyphal formation in Candida albicans by mutation of a STE12 homolog. Science 266 (1994) 1723-1726
27. Lopez-Ribot J.L., Casanova M., Murgui A., and Martinez J.P. Antibody response to Candida albicans cell wall antigens. FEMS Immunol. Med. Microbiol. 41 (2004) 187-196
28. Maclennan A.J., and Shaw G. A yeast SH2 domain. Trends Biochem. Sci. 18 (1993) 464-465
29. Mumberg D., Muller R., and Funk M. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22 (1994) 5767-5768
30. Nguyen M.H., Cheng S., and Clancy C.J. Assessment of Candida albicans genes expressed during infections as a tool to understand pathogenesis. Med. Mycol. 42 (2004) 293-304
31. Noble S.M., and Johnson A.D. Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans. Eukaryot. Cell 4 (2005) 298-309
32. Pitarch A., Jimenez A., Nombela C., and Gil C. Decoding serological response to Candida cell wall immunome into novel diagnostic, prognostic, and therapeutic candidates for systemic candidiasis by proteomic and bioinformatic analyses. Mol. Cell Proteom. 5 (2006) 79-96
33. Reuβ O., Vik A., Kolter R., and Morschhauser J. The SAT1 flipper, an optimized tool for gene disruption in Candida albicans. Gene 341 (2004) 119-127
34. Sambrook J., and Russell D. Molecular Cloning: A Laboratory Manual (2001), Cold Spring Harbor Laboratory Press, Woodbury, NY
35. San-Blas G., Travassos L.R., Fries B.C., Goldman D.L., Casadevall A., Carmona A.K., Barros T.F., Puccia R., Hostetter M.K., Shanks S.G., Copping V.M., Knox Y., and Gow N.A. Fungal morphogenesis and virulence. Med. Mycol. 38 Suppl. 1 (2000) 79-86
36. Stoldt V.R., Sonneborn A., Leuker C.E., and Ernst J.F. Efg1p, an essential regulator of morphogenesis of the human pathogen Candida albicans, is a member of a conserved class of bHLH proteins regulating morphogenetic processes in fungi. EMBO J. 16 (1997) 1982-1991
37. Sundstrom P., and Aliaga G.R. A subset of proteins found in culture supernatants of Candida albicans includes the abundant, immunodominant, glycolytic enzyme enolase. J. Infect. Dis. 169 (1994) 452-456
38. Swanson M.S., Carlson M., and Winston F. SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus. Mol. Cell. Biol. 10 (1990) 4935-4941
39. Thomas D.P., Viudes A., Monteagudo C., Lazzell A.L., Saville S.P., and Lopez-Ribot J.L. A proteomic-based approach for the identification of Candida albicans protein components present in a subunit vaccine that protects against disseminated candidiasis. Proteomics 6 (2006) 6033-6041
40. Uhl M.A., Biery M., Craig N., and Johnson A.D. Haploinsufficiency-based large-scale forward genetic analysis of filamentous growth in the diploid human fungal pathogen C. albicans. EMBO J. 22 (2003) 2668-2678
41. Urban C., Sohn K., Lottspeich F., Brunner H., and Rupp S. Identification of cell surface determinants in Candida albicans reveals Tsa1p, a protein differentially localized in the cell. FEBS Lett. 544 (2003) 228-235
42. Viudes A., Peman J., Canton E., Ubeda P., Lopez-Ribot J.L., and Gobernado M. Candidemia at a tertiary-care hospital: epidemiology, treatment, clinical outcome and risk factors for death. Eur. J. Clin. Microbiol. Infect. Dis. 21 (2002) 767-774
43. Wellington M., Bliss J.M., and Haidaris C.G. Enhanced phagocytosis of Candida species mediated by opsonization with a recombinant human antibody single-chain variable fragment. Infect. Immun. 71 (2003) 7228-7231
44. Winston F., Chaleff D.T., Valent B., and Fink G.R. Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107 (1984) 179-197
45. Zakikhany K., Naglik J.R., Schmidt-Westhausen A., Holland G., Schaller M., and Hube B. In vivo transcript profiling of Candida albicans identifies a gene essential for interepithelial dissemination. Cell Microbiol. 9 (2007) 2938-2954