Detection of CFTR transgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity

Journal of Gene Medicine

Volumn 12, Issue 1, 2010, Pages 55-63

  Holder, Emma ^a,d   Stevenson, Barbara ^a,d   Farley, Raymond ^b,d   Hilliard, Tom ^b,d   Wodehouse, Theresa ^b,d   Somerton, Lucinda ^b,d   Larsen, Mia ^b,d   O'Donoghue, Jean ^a   Coles, Rebecca L ^c,d   Scheule, Ronald K ^e   Cheng, Seng H ^e   Gill, Deborah R ^c,d   Hyde, Stephen C ^c,d   Griesenbach, Uta ^b,d   Alton, Eric W F W ^b,d   Porteous, David J ^a,d   Christopher Boyd, A ^a,d  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^c UNIVERSITY OF OXFORD   (United Kingdom)

^d UK Cystic Fibrosis Gene Therapy Consortium   (United Kingdom)

^e GENZYME CORPORATION   (United States)

Author keywords

CFTR; Gene transfer; Laser microdissection; Nasal brushing; Pronase digestion; Respiratory epithelium

Indexed keywords

MESSENGER RNA; PRONASE; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CYSTIC FIBROSIS; EPITHELIUM CELL; GENE EXPRESSION; GENE SEQUENCE; GENE THERAPY; GENE TRANSFER; GENETIC TRANSCRIPTION; KNOCKOUT MOUSE; LASER MICRODISSECTION; MOUSE; NONHUMAN; NOSE CAVITY; PRIORITY JOURNAL; RESPIRATORY EPITHELIUM; TRANSGENE; WILD TYPE;

ANIMALS; CELL SEPARATION; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; EPITHELIAL CELLS; FATTY ACID-BINDING PROTEINS; GENE EXPRESSION REGULATION; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; LASERS; MICE; MICE, INBRED C57BL; MICRODISSECTION; NASAL CAVITY; NASAL SEPTUM; PRONASE; RESPIRATORY MUCOSA; RNA, MESSENGER; TRANSGENES;

MURINAE; MUS;

EID: 75449114021     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.1413     Document Type: Article

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