Targeting the A site RNA of the Escherichia coli ribosomal 30 S subunit by 2′-O-methyl oligoribonucleotides: A quantitative equilibrium dialysis binding assay and differential effects of aminoglycoside antibiotics

Biochemical Journal

Volumn 383, Issue 2, 2004, Pages 201-208

  Abelian, Arthur ^a,c   Walsh, Andrew P ^a,d   Lentzen, Georg ^b,e   Aboul Ela, Fareed ^b   Gait, Michael J ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b Vernalis PLC   (United Kingdom)

^c NORTH MIDDLESEX UNIVERSITY HOSPITAL   (United Kingdom)

^d UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^e BITOP AG   (Germany)

Author keywords

Aminoglycoside; Antibiotic; Equilibrium dialysis; Oligonucleotide; Ribosome; Translation inhibition

Indexed keywords

AMINO ACIDS; ANTIBIOTICS; BINDING ENERGY; DIALYSIS; DISSOCIATION; ESCHERICHIA COLI; SYNTHESIS (CHEMICAL);

ANTIBACTERIAL AGENTS; OLIGONEUCLEOTIDES; PAROMOMYCIN; RIBOSOME;

RNA;

2' O METHYL OLIGORIBONUCLEOTIDE; ADENINE; AMINOACYL TRANSFER RNA; AMINOGLYCOSIDE ANTIBIOTIC AGENT; HYGROMYCIN B; OLIGORIBONUCLEOTIDE; PAROMOMYCIN; RNA 16S; TRANSFER RNA; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING ASSAY; BINDING COMPETITION; CONFORMATIONAL TRANSITION; DISSOCIATION CONSTANT; DRUG BINDING; DRUG BINDING SITE; EQUILIBRIUM DIALYSIS; ESCHERICHIA COLI; LETHALITY; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; QUANTITATIVE ASSAY; QUANTITATIVE EQUILIBRIUM DIALYSIS BINDING ASSAY; RIBOSOME SUBUNIT; RNA BINDING; TRANSLATION REGULATION;

AMINOGLYCOSIDES; ANTI-BACTERIAL AGENTS; BINDING, COMPETITIVE; DIALYSIS; ESCHERICHIA COLI; MODELS, MOLECULAR; OLIGORIBONUCLEOTIDES; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; PROTEIN SUBUNITS; RIBOSOMES; RNA, RIBOSOMAL; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 7444247816     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20040246     Document Type: Article

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