A gas chromatography-mass spectrometry method for the measurement of fatty acid ω and ω-1 hydroxylation kinetics by CYP4A1 using an artificial membrane system

Analytical Biochemistry

Volumn 325, Issue 2, 2004, Pages 354-363

  Holmes, Victoria E ^a   Bruce, Mary ^a   Shaw, P Nicholas ^a   Bell, David R ^a   Qi, Fan Ming ^a   Barrett, David A ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Artificial membrane; CYP4A1; Fatty acids; GC MS; Hydroxylation; Kinetics

Indexed keywords

ENZYME KINETICS; ENZYMES; FATTY ACIDS; HYDROXYLATION; KINETICS; MASS SPECTROMETRY; METABOLITES; MOLAR RATIO; PALMITIC ACID; PHASE SEPARATION;

ARTIFICIAL MEMBRANES; CYP4A1; CYTOCHROME P450 REDUCTASE; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHODS; GC-MS; HYDROXYLATED METABOLITES; SOLID-PHASE EXTRACTION;

GAS CHROMATOGRAPHY;

CYTOCHROME B5; CYTOCHROME P450 4A; CYTOCHROME P450 4A1; CYTOCHROME P450 REDUCTASE; LAURIC ACID; MYRISTIC ACID; PALMITIC ACID; UNCLASSIFIED DRUG;

ACCURACY; ANIMAL TISSUE; ARTICLE; ARTIFICIAL MEMBRANE; CONTROLLED STUDY; DERIVATIZATION; ENZYME PURIFICATION; FATTY ACID METABOLISM; GAS CHROMATOGRAPHY; HYDROXYLATION; IN VITRO STUDY; LIVER MICROSOME; MASS SPECTROMETRY; MICROSOME MEMBRANE; NONHUMAN; PRIORITY JOURNAL; RAT; SOLID PHASE EXTRACTION;

EID: 1642538403     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.10.046     Document Type: Article

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