Gelatin in situ zymography on fixed, paraffin-embedded tissue: Zinc and ethanol fixation preserve enzyme activity

Journal of Histochemistry and Cytochemistry

Volumn 58, Issue 1, 2010, Pages 29-39

  Hadler Olsen, Elin ^a   Kanapathippillai, Premasany ^a   Berg, Eli ^a   Svineng, Gunbjørg ^a   Winberg, Jan Olof ^a   Uhlin Hansen, Lars ^a,b  

^a UNIVERSITY OF TROMSØ   (Norway)

^b UNIVERSITY HOSPITAL OF NORTH NORWAY   (Norway)

Author keywords

Extraction; Fixation; In situ zymography; Matrix metalloproteases; Morphology

Indexed keywords

ALCOHOL; BUFFER; FIXATIVE; GELATIN; GELATINASE A; GELATINASE B; PARAFFIN; ZINC; COLORING AGENT; DIAGNOSTIC AGENT; ENZYME PRECURSOR; PEPTIDE HYDROLASE;

ACCURACY; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; EXTRACTION; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; TISSUE FIXATION; WESTERN BLOTTING; ZYMOGRAPHY; ANIMAL; BAGG ALBINO MOUSE; CHEMISTRY; CYTOLOGY; DNA MICROARRAY; GENETICS; HEART MUSCLE; IMMUNOHISTOCHEMISTRY; KIDNEY; LIVER; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; NUDE MOUSE; ORGAN PRESERVATION; TONGUE;

ANIMALS; COLORING AGENTS; ENZYME PRECURSORS; ETHANOL; GELATIN; IMMUNOHISTOCHEMISTRY; KIDNEY; LIVER; MASS SPECTROMETRY; MATRIX METALLOPROTEINASE 2; MATRIX METALLOPROTEINASE 9; MICE; MICE, INBRED BALB C; MICE, NUDE; MYOCARDIUM; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; ORGAN PRESERVATION; PARAFFIN EMBEDDING; PEPTIDE HYDROLASES; TISSUE FIXATION; TONGUE; ZINC;

EID: 74049105606     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2009.954354     Document Type: Article

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