Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing of human tissues

Laboratory Investigation

Volumn 83, Issue 6, 2003, Pages 889-899

  Wester, Kenneth ^a,d   Asplund, Anna ^a   Bäckvall, Helena ^a   Micke, Patrick ^b   Derveniece, Andra ^c   Hartmane, Ilona ^c   Malmström, Per Uno ^a   Pontén, Fredrik ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b LUDWIG INSTITUTE FOR CANCER RESEARCH   (Sweden)

^c RIGA STRADINS UNIVERSITY   (Latvia)

^d UPPSALA UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 MICROGLOBULIN; FIXATIVE; FORMALDEHYDE; GENOMIC DNA; NEUTRAL BUFFERED FORMALIN; PARAFFIN; PRIMER DNA; PROTEIN; RNA; TRANSFERRIN RECEPTOR; UNCLASSIFIED DRUG; ZINC BASED FIXATIVE; ZINC DERIVATIVE;

ARTICLE; CRYOPRESERVATION; DISSECTION; DNA DETERMINATION; DNA MICROARRAY; FREEZING; GENE EXPRESSION; HISTOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; LASER MICRODISSECTION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; RNA ANALYSIS; SNAP FREEZING; TISSUE; TISSUE FIXATION; TISSUE MICROARRAY; TISSUE PRESERVATION;

EID: 0038001700     PISSN: 00236837     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.LAB.0000074892.53211.A5     Document Type: Article

References (27)

1. Arnold MM, Srivastava S, Fredenburgh J, Stockard CR, Myers RB, and Grizzle WE (1996). Effects of fixation and tissue processing on immunohistochemical demonstration of specific antigens. Biotech Histochem 71:224-230.
2. Baker JR (1958). Principles of biological microtechnique. A study of fixation and dyeing, 1st ed. London: Methuen & Co. Ltd.
3. Beckstead JH (1994). A simple technique for preservation of fixation-sensitive antigens in paraffin-embedded tissues. J Histochem Cytochem 42:1127-1134.
4. Brenes F, Harris S, Paz MO, Petrovic LM, and Scheuer PJ (1986). PLP fixation for combined routine histology and immunocytochemistry of liver biopsies. J Clin Pathol 39:459-463.
5. Crisan D and Mattson JC (1993). Retrospective DNA analysis using fixed tissue specimens. DNA Cell Biol 12:455-464.
6. Garnis-Jones S and Mah R (1988). Aceton fixation preserves melanoma-associated antigens in paraffin-embedded tissue. J Tu Marker Oncol 3:467-473.
7. Goldsworthy SM, Stockton PS, Trempus CS, Foley JF, and Maronpot RR (1999). Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol Carcinog 25:86-91.
8. Gonzalez L, Anderson I, Deane D, Summers C, and Buxton D (2001). Detection of immune system cells in paraffin wax-embedded ovine tissues. J Comp Pathol 125:41-47.
9. Greer CE, Lund JK, and Manos MM (1991). PCR amplification from paraffin-embedded tissues: Recommendations on fixatives for long-term storage and prospective studies. PCR Methods Appl 1:46-50.
10. Greer CE, Wheeler CM, and Manos MM (1994). Sample preparation and PCR amplification from paraffin-embedded tissues. PCR Methods Appl 3 (Suppl):113-122.
11. Herman GE, Chlipala E, Bochenski G, Sabin L, and Elfont E (1988). Zinc formalin fixative for automated tissue processing. J Histotechnol 11:85-89.
12. Huang SN, Minassian H, and More JD (1976). Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. Lab Invest 35:383-390.
13. Johansson M, Jansson T, and Powell TL (2000). Na(+)-K(+)-ATPase is distributed to microvillous and basal membrane of the syncytiotrophoblast in human placenta. Am J Physiol Regul Integr Comp Physiol 279:R287-294.
14. Lalani EN, Golding M, Hudson M, Chieffi G, Stamp G, Anilkumar TV, Sarraf C, and Alison MR (1995). Protein extraction and western blotting from methacarn-fixed tissue. J Pathol 177:323-328.
15. Ling G, Persson A, Berne B, Uhlen M, Lundeberg J, and Ponten F (2001). Persistent p53 mutations in single cells from normal human skin. Am J Pathol 159:1247-1253.
16. Mason JT and O'Leary TJ (1991). Effects of formaldehyde fixation on protein secondary structure: A calorimetric and infrared spectroscopic investigation. J Histochem Cytochem 39:225-229.
17. Nitta H, Munger W, Wilson E, Ralston R, and Alila H (1997). Improved in situ immunodetection of leukocytes on paraffin-embedded mouse spleen. Cell Vis 4:73-80.
18. Persson AE, Ling G, Williams C, Backvall H, Ponten J, Ponten F, and Lundeberg J (2000). Analysis of p53 mutations in single cells obtained from histological tissue sections. Anal Biochem 287:25-31.
19. Scheidl SJ, Nilsson S, Kalen M, Hellstrom M, Takemoto M, Hakansson J, and Lindahl P (2002). mRNA expression profiling of laser microbeam microdissected cells from slender embryonic structures. Am J Pathol 160:801-813.
20. Schutze K and Clement-Sengewald A (1994). Catch and move-cut or fuse. Nature 368:667-669.
21. Shi SR, Key ME, and Kalra KL (1991). Antigen retrieval in formalin-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741-748.
22. Shibutani M, Uneyama C, Miyazaki K, Toyoda K, and Hirose M (2000). Methacarn fixation: A novel tool for analysis of gene expressions in paraffin-embedded tissue specimens. Lab Invest 80:199-208.
23. Tome Y, Hirohashi S, Noguchi M, and Shimosato Y (1990). Preservation of cluster 1 small cell lung cancer antigen in zinc-formalin fixative and its application to immunohistological diagnosis. Histopathology 16:469-474.
24. Wang BL, Springer GF, and Kaufman MW (1996). Concurrent immunohistochemical staining of tumor-infiltrating lymphocytes and carcinoma-associated T (Thomsen-Friedenreich)/Tn antigens in human breast carcinoma. J Histochem Cytochem 44:187-191.
25. Warford A, Pringle JH, Hay J, Henderson SD, and Lauder I (1988). Southern blot analysis of DNA extracted from formalsaline fixed and paraffin wax embedded tissue. J Pathol 154:313-320.
26. Weaver DL, Mitchell J, and Leslie KO (1992). Effects of four primary fixatives on the sensitivity of immunohistochemical staining. J Histotechnol 15:27-30.
27. Williams C, Ponten F, Moberg C, Soderkvist P, Uhlen M, Ponten J, Sitbon G, and Lundeberg J (1999). A high frequency of sequence alterations is due to formalin fixation of archival specimens. Am J Pathol 155:1467-1471.