Macromolecular changes caused by formalin fixation and antigen retrieval

Biotechnic and Histochemistry

Volumn 82, Issue 3, 2007, Pages 133-140

  Dapson, R W ^a  

^a Dapson & Dapson LLC   (United States)

Author keywords

Antigen retrieval; Fixation; Formaldehyde; Formalin; Immunohistochemistry; Molecular conformation

Indexed keywords

EPITOPE; FORMALDEHYDE; IMINE;

ANTIGEN RETRIEVAL; CHEMICAL BINDING; CROSS LINKING; DEHYDRATION; DNA ADDUCT; DNA DAMAGE; HYDROPHOBICITY; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MACROMOLECULE; MOLECULAR STABILITY; PH; PROTEIN DEGRADATION; PROTEIN STRUCTURE; REVIEW; TEMPERATURE DEPENDENCE; TISSUE FIXATION; TISSUE REACTION;

CROSS-LINKING REAGENTS; DNA ADDUCTS; EPITOPES; FIXATIVES; FORMALDEHYDE; IMMUNOHISTOCHEMISTRY; PROTEIN CONFORMATION; TISSUE FIXATION;

EID: 36048989692     PISSN: 10520295     EISSN: 14737760     Source Type: Journal    
DOI: 10.1080/10520290701567916     Document Type: Review

References (21)

1. Brandon, C. and Tooze, J. (1991) Introduction to Protein Structure, p. 302 pp. Garland Publishing, New York
2. Bridson, PK, Jiricny, J., Kemal, O. and Reese, CB (1980) Reactions between ribonucleoside derivatives and formaldehyde in ethanol solution. J. Chem. Soc. Chem. Commun, 5, pp. 208-209.
3. Clinical and Laboratory Standards Institute (1999) Quality Assurance for Immunocytochemistry; Approved Guideline, p. 72 pp.
4. Dapson, RW (1993) Fixation for the 1990s: A review of needs and accomplishments. Biotech. Histochem, 68, pp. 75-82.
5. Dapson, RW, Feldman, AT and Wolfe, D. (2005) Lessons in Immunohistochemistry, p. 28 pp.
6. Fox, CH, Johnson, FB, Whiting, J. and Roller, PP (1985) Formaldehyde fixation. J. Histochem. Cytochem, 33, pp. 845-853.
7. Fraenkel-Conrat, H. and Singer, B. (1988) Nucleoside adducts are formed by cooperative reaction of acetaldehyde and alcohols: Possible mechanism for the role of ethanol in carcinogenesis. Proc. Natl. Acad. Sci. USA, 85, pp. 3758-3761.
8. French, D. and Edsall, JT (1945) The reactions of formaldehyde with amino acids and proteins. Adv. Prot.Chem, 2, pp. 277-335.
9. Haselkorn, R. and Doty, P. (1961) The reaction of formaldehyde with poynucleotides. J. Biol. Chem, 236, pp. 2738-2745.
10. Mason, JT and O'Leary, TJ (1991) Effects of formaldehyde fixation on protein secondary structure: A calorimetric and infrared spectroscopic investigation. J. Histochem. Cytochem, 39, pp. 225-229.
11. McGhee, JD and von Hippek, PH (1975) Formaldehyde as a probe of DNA structure. I. Reaction with exocyclic amino groups of DNA bases. Biochemistry, 14, pp. 1281-1296.
12. Namimatsu, S., Ghazizadeh, M. and Sugiski, Y. (2005) Revering the effects of formalin fixation with citraconic anhydride and heat: A universal antigen retrieval method. J. Histochem. Cytochem, 53, pp. 3-11.
13. Pearse AGE (1968) Histochemistry, Theoretical and Applied, 1, pp. 70-73. Little Brown & Co., Boston, MA
14. Puchtler, H. and Meloan, SN (1985) On the chemistry of formaldehyde fixation and its effects on immunohistochemical reactions. Histochemistry, 82, pp. 201-204.
15. Rait, VK, Zhang, Q., Fabris, D., Mason, J. and O'Leary, T. (2006) Conversions of formaldehyde-modified 2′-deoxyadenosine 5′-monophosphate in conditions modeling formalin-fixed tissue dehydration. J. Histochem. Cytochem, 54, pp. 301-310.
16. Shi, S-R, Cote, RJ and Taylor, CR (1997) Antigen retrieval immunohistochemistry: Past, present, and future. J. Histochem. Cytochem, 45, pp. 327-343.
17. Shi, S-R, Cote, RJ, Shi, Y. and Taylor, CR (2000) Antigen retrieval techniques (Appendix). Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology, pp. 311-333. Eaton Publishing, Natick, MA
18. Shi, S-R, Cote, RJ and Taylor, CR (2001) Antigen retrieval techniques: current perspectives. J. Histochem. Cytochem, 49, pp. 931-937.
19. Solomon, MJ and Varshavsky, A. (1985) formaldehyde-mediated DNA-protein crosslinking: A probe for in vivo chromatin structures. Proc. Natl. Acad. Sci. USA, 82, pp. 6470-6474.
20. Srinivasan, M., Sedmak, D. and Jewell, S. (2002) Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am. J. Pathol, 161, pp. 1961-1971.
21. Walker, JF (1964) Formaldehyde, Reinhold, New York