-
1
-
-
0034614490
-
Signaling—2000 and beyond
-
Hunter, T. Signaling—2000 and beyond. Cell 100, 113-127 (2000).
-
(2000)
Cell
, vol.100
, pp. 113-127
-
-
Hunter, T.1
-
2
-
-
0037032835
-
The protein kinase complement of the human genome
-
Manning, G., Whyte, D.B., Martinez, R., Hunter, T. & Sudarsanam, S. The protein kinase complement of the human genome. Science 298, 1912-1934 (2002).
-
(2002)
Science
, vol.298
, pp. 1912-1934
-
-
Manning, G.1
Whyte, D.B.2
Martinez, R.3
Hunter, T.4
Sudarsanam, S.5
-
3
-
-
18744415995
-
Kinomics: Methods for deciphering the kinome
-
Johnson, S.A. & Hunter, T. Kinomics: methods for deciphering the kinome. Nat. Methods 2, 17-25 (2005).
-
(2005)
Nat. Methods
, vol.2
, pp. 17-25
-
-
Johnson, S.A.1
Hunter, T.2
-
4
-
-
0036527429
-
Protein kinase—the majordrug targets ofthe twenty-first century?
-
Cohen, P. Protein kinase—the majordrug targets ofthe twenty-first century? Nat. Rev. Drug Discov. 1, 309-315 (2002).
-
(2002)
Nat. Rev. Drug Discov
, vol.1
, pp. 309-315
-
-
Cohen, P.1
-
5
-
-
0014949207
-
Cleavage ofstructural proteins during the assembly of the head of bacteriophage T4
-
Laemmli, U.K. Cleavage ofstructural proteins during the assembly of the head of bacteriophage T4. Nature 221, 680-685 (1970).
-
(1970)
Nature
, vol.221
, pp. 680-685
-
-
Laemmli, U.K.1
-
6
-
-
12444315072
-
Global quantitative phosphoprotein analysis using multiplexed proteomics technology
-
Steinberg, T.H. et al. Global quantitative phosphoprotein analysis using multiplexed proteomics technology. Proteomics 3, 1128-1144 (2003).
-
(2003)
Proteomics
, vol.3
, pp. 1128-1144
-
-
Steinberg, T.H.1
-
7
-
-
4444337889
-
Detection of phosphoproteins on electroblot membranes using a small-molecule organic fluorophore
-
Goodman, T., Schulenberg, B., Steinberg, T.H. & Patton, W.F. Detection of phosphoproteins on electroblot membranes using a small-molecule organic fluorophore. Electrophoresis 25, 2533-2538 (2004).
-
(2004)
Electrophoresis
, vol.25
, pp. 2533-2538
-
-
Goodman, T.1
Schulenberg, B.2
Steinberg, T.H.3
Patton, W.F.4
-
8
-
-
44449166482
-
Blue light-induced autophosphorylation of phototropin is a primary step for signaling
-
Inoue, S. et al. Blue light-induced autophosphorylation of phototropin is a primary step for signaling. Proc. Natl. Acad. Sci. USA 105, 5626-5631 (2008).
-
(2008)
Proc. Natl. Acad. Sci. USA
, vol.105
, pp. 5626-5631
-
-
Inoue, S.1
-
9
-
-
41549148228
-
Universally applicable methods for monitoring response regulator aspartate phosphorylation both in vitro and in vivo using Phos- tag-based reagents
-
Barbieri, C.M. & Stock, A.M. Universally applicable methods for monitoring response regulator aspartate phosphorylation both in vitro and in vivo using Phos- tag-based reagents. Anal. Biochem. 376, 73-82 (2008).
-
(2008)
Anal. Biochem
, vol.376
, pp. 73-82
-
-
Barbieri, C.M.1
Stock, A.M.2
-
10
-
-
44249090886
-
Identification of phosphotyrosyl proteins in vitreous humours of patients with vitreoretinal diseases by sodium dodecyl sulphate-polyacrylamide gel electrophoresis/western blotting/matrix-assisted laser desorption time-of-flight mass spectrometry
-
Mukai, N., Nakanishi, T., Shimizu, A., Takubo, T. & Ikeda, T. Identification of phosphotyrosyl proteins in vitreous humours of patients with vitreoretinal diseases by sodium dodecyl sulphate-polyacrylamide gel electrophoresis/western blotting/matrix-assisted laser desorption time-of-flight mass spectrometry. Ann. Clin. Biochem. 45, 307-312 (2008).
-
(2008)
Ann. Clin. Biochem
, vol.45
, pp. 307-312
-
-
Mukai, N.1
Nakanishi, T.2
Shimizu, A.3
Takubo, T.4
Ikeda, T.5
-
11
-
-
55449092939
-
Glycogen synthase kinase 3 inhibition slows mitochondrial adenine nucleotide transport and regulates voltage-dependent anion channel phosphorylation. Circ
-
Das, S., Wong, R., Rajapakse, N., Murphy, E. & Steenbergen, C. Glycogen synthase kinase 3 inhibition slows mitochondrial adenine nucleotide transport and regulates voltage-dependent anion channel phosphorylation. Circ. Res. 103, 983-991 (2008).
-
(2008)
Res
, vol.103
, pp. 983-991
-
-
Das, S.1
Wong, R.2
Rajapakse, N.3
Murphy, E.4
Steenbergen, C.5
-
12
-
-
67049098588
-
Use of 32P to study dynamics of the mitochondrial phosphoproteome
-
Aponte, A.M. et al. Use of 32P to study dynamics of the mitochondrial phosphoproteome. J. Proteome Res. 8, 2679-2695 (2009).
-
(2009)
J. Proteome Res
, vol.8
, pp. 2679-2695
-
-
Aponte, A.M.1
-
13
-
-
49749111773
-
Immobilized metal affinity electrophoresis: A novel method of capturing phosphoproteins by electrophoresis
-
Lee, B.S., Lasanthi, G.D., Jayathilaka, G.D., Huang, J.S. & Gupta, S. Immobilized metal affinity electrophoresis: a novel method of capturing phosphoproteins by electrophoresis. J. Biomol. Tech. 19, 106-108 (2008).
-
(2008)
J. Biomol. Tech
, vol.19
, pp. 106-108
-
-
Lee, B.S.1
Lasanthi, G.D.2
Jayathilaka, G.D.3
Huang, J.S.4
Gupta, S.5
-
14
-
-
49749133835
-
Modification of the immobilized metal affinity electrophoresis using sodium dodecyl sulfate polyacrylamide gel electrophoresis
-
Lee, B.S. et al. Modification of the immobilized metal affinity electrophoresis using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Electrophoresis 29, 3160-3163 (2008).
-
(2008)
Electrophoresis
, vol.29
, pp. 3160-3163
-
-
Lee, B.S.1
-
15
-
-
2342589413
-
Recognition of phosphate monoester dianion by an alkoxide-bridged dinuclear zinc(II) complex
-
Kinoshita, E., Takahashi, M., Takeda, H., Shiro, M. & Koike, T Recognition of phosphate monoester dianion by an alkoxide-bridged dinuclear zinc(II) complex. Dalton Trans. 1189-1193 (2004).
-
(2004)
Dalton Trans
, pp. 1189-1193
-
-
Kinoshita, E.1
Takahashi, M.2
Takeda, H.3
Shiro, M.4
Koike, T.5
-
16
-
-
33645714857
-
Phosphate-binding tag, a new tool to visualize phosphorylated proteins
-
Kinoshita, E., Kinoshita-Kikuta, E., Takiyama, K. & Koike, T. Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Mol. Cell. Proteomics 5,749-757 (2006).
-
(2006)
Mol. Cell. Proteomics
, vol.5
, pp. 749-757
-
-
Kinoshita, E.1
Kinoshita-Kikuta, E.2
Takiyama, K.3
Koike, T.4
-
17
-
-
33847688584
-
Label-free kinase profiling using phosphate affinity polyacrylamide gelelectrophoresis
-
Kinoshita-Kikuta, E., Aoki, Y., Kinoshita, E. & Koike, T. Label-free kinase profiling using phosphate affinity polyacrylamide gelelectrophoresis. Mol. Cell. Proteomics 6, 356-366 (2007).
-
(2007)
Mol. Cell. Proteomics
, vol.6
, pp. 356-366
-
-
Kinoshita-Kikuta, E.1
Aoki, Y.2
Kinoshita, E.3
Koike, T.4
-
18
-
-
49749115736
-
Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE
-
Kinoshita, E. et al. Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE. Proteomics 8, 2994-3003 (2008).
-
(2008)
Proteomics
, vol.8
, pp. 2994-3003
-
-
Kinoshita, E.1
-
19
-
-
64049105194
-
Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes
-
Kinoshita, E. et al. Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes. Electrophoresis 30, 550-559 (2009).
-
(2009)
Electrophoresis
, vol.30
, pp. 550-559
-
-
Kinoshita, E.1
-
20
-
-
67650908878
-
Phosphate-affinity gel electrophoresis using a Phos-tag molecule for phosphoproteome study
-
Kinoshita, E., Kinoshita-Kikuta, E. & Koike, T. Phosphate-affinity gel electrophoresis using a Phos-tag molecule for phosphoproteome study. Curr. Proteomics 6, 104-121 (2009).
-
(2009)
Curr. Proteomics
, vol.6
, pp. 104-121
-
-
Kinoshita, E.1
Kinoshita-Kikuta, E.2
Koike, T.3
-
21
-
-
33845423867
-
Separation of a phosphorylated histidine protein using phosphate affinity polyacrylamide gel electrophoresis
-
Yamada, S. et al. Separation of a phosphorylated histidine protein using phosphate affinity polyacrylamide gel electrophoresis. Anal. Biochem. 360, 160-162 (2007).
-
(2007)
Anal. Biochem
, vol.360
, pp. 160-162
-
-
Yamada, S.1
-
22
-
-
35448998917
-
Analysis ofthe CK2-dependent phosphorylation ofserine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis
-
Miyata, Y. & Nishida, E. Analysis ofthe CK2-dependent phosphorylation ofserine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis. FEBS J. 274, 5690-5703 (2007).
-
(2007)
FEBS J
, vol.274
, pp. 5690-5703
-
-
Miyata, Y.1
Nishida, E.2
-
23
-
-
35748968807
-
Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling
-
Maeder, C.I. et al. Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat. Cell Biol. 9, 1319-1326 (2007).
-
(2007)
Nat. Cell Biol
, vol.9
, pp. 1319-1326
-
-
Maeder, C.I.1
-
25
-
-
48949120193
-
Coimmunopurification of phosphorylated bacterial- and plant- type phosphoenolpyruvate carboxylases with the plastidial pyruvate dehydrogenase complex from developing castor oil seeds
-
Uhrig, R.G. et al. Coimmunopurification of phosphorylated bacterial- and plant- type phosphoenolpyruvate carboxylases with the plastidial pyruvate dehydrogenase complex from developing castor oil seeds. Plant Physiol. 146, 1346-1357 (2008).
-
(2008)
Plant Physiol
, vol.146
, pp. 1346-1357
-
-
Uhrig, R.G.1
-
26
-
-
48249157879
-
A highly sensitive technique to measure myosin regulatory light chain phosphorylation: The first quantification in renal arterioles
-
Takeya, K., Loutzenhiser, K., Shiraishi, M., Loutzenhiser, R. & Walsh, M.P. A highly sensitive technique to measure myosin regulatory light chain phosphorylation: the first quantification in renal arterioles. Am. J. Physiol. Renal Physiol. 294, F1487-F1492 (2008).
-
(2008)
Am. J. Physiol. Renal Physiol
, vol.294
, pp. F1487-F1492
-
-
Takeya, K.1
Loutzenhiser, K.2
Shiraishi, M.3
Loutzenhiser, R.4
Walsh, M.P.5
-
27
-
-
45549091754
-
Identification of ubiquitin ligase activity of RBCK1 and its inhibition by splice variant RBCK2 and protein kinase Cp
-
Tatematsu, K., Yoshimoto, N., Okajima, T., Tanizawa, K. & Kuroda, S. Identification of ubiquitin ligase activity of RBCK1 and its inhibition by splice variant RBCK2 and protein kinase Cp. J. Biol. Chem. 283, 11575-11585 (2008).
-
(2008)
J. Biol. Chem
, vol.283
, pp. 11575-11585
-
-
Tatematsu, K.1
Yoshimoto, N.2
Okajima, T.3
Tanizawa, K.4
Kuroda, S.5
-
28
-
-
48249129062
-
Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation ofyeast Mcm4 N-terminal domain
-
Devault, A., Gueydon, E. & Schwob, E. Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation ofyeast Mcm4 N-terminal domain. Mol. Biol. Cell 19,2267-2277 (2008).
-
(2008)
Mol. Biol. Cell
, vol.19
, pp. 2267-2277
-
-
Devault, A.1
Gueydon, E.2
Schwob, E.3
-
29
-
-
44449172019
-
The wheat germ cell-free based screening of protein substrates of calcium/calmodulin-dependent protein kinase II delta
-
Masaoka, T., Nishi, M., Ryo, A., Endo, Y. & Sawasaki, T. The wheat germ cell-free based screening of protein substrates of calcium/calmodulin-dependent protein kinase II delta. FEBSLett. 582, 1795-1801 (2008).
-
(2008)
Febslett
, vol.582
, pp. 1795-1801
-
-
Masaoka, T.1
Nishi, M.2
Ryo, A.3
Endo, Y.4
Sawasaki, T.5
-
30
-
-
54449099430
-
Virusinfection triggersSUMOylation ofIRF3 and IRF7, leading to the negative regulation of type I interferon gene expression
-
Kubota, T. et al. Virusinfection triggersSUMOylation ofIRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J. Biol. Chem. 283, 25660-25670 (2008).
-
(2008)
J. Biol. Chem
, vol.283
, pp. 25660-25670
-
-
Kubota, T.1
-
31
-
-
55449117866
-
Dbf4-dependent Cdc7 kinase links DNA replication to the segregation of homologous chromosomes in meiosis I
-
Matos, J. et al. Dbf4-dependent Cdc7 kinase links DNA replication to the segregation of homologous chromosomes in meiosis I. Cell 135, 662-678 (2008).
-
(2008)
Cell
, vol.135
, pp. 662-678
-
-
Matos, J.1
-
32
-
-
66049110566
-
Flg22 regulates the release of an ethylene response factor substrate from MAP kinase 6 in Arabidopsis thaliana via ethylene signaling
-
Bethke, G. et al. Flg22 regulates the release of an ethylene response factor substrate from MAP kinase 6 in Arabidopsis thaliana via ethylene signaling. Proc. Natl. Acad. Sci. USA 106, 8067-8072 (2009).
-
(2009)
Proc. Natl. Acad. Sci. USA
, vol.106
, pp. 8067-8072
-
-
Bethke, G.1
-
33
-
-
65449145956
-
Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor
-
Hanyu, Y. et al. Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor. Genes Cells 14, 539-554 (2009).
-
(2009)
Genes Cells
, vol.14
, pp. 539-554
-
-
Hanyu, Y.1
-
34
-
-
55549137026
-
FANCI phosphorylation functions asa molecularswitch to turn on the Fanconi anemia pathway
-
Ishiai, M. et al. FANCI phosphorylation functions asa molecularswitch to turn on the Fanconi anemia pathway. Nat. Struct. Mol. Biol. 15, 1138-1146 (2008).
-
(2008)
Nat. Struct. Mol. Biol
, vol.15
, pp. 1138-1146
-
-
Ishiai, M.1
-
35
-
-
69449103922
-
Detection of phosphorylation on large proteins by Western blotting using Phos-tag containing gel
-
Tomida, J., Kitao, H., Kinoshita, E. & Takata, M. Detection of phosphorylation on large proteins by Western blotting using Phos-tag containing gel. Nat. Protoc. doi:10.1038/nprot.2008.232 (2008).
-
(2008)
Nat. Protoc
-
-
Tomida, J.1
Kitao, H.2
Kinoshita, E.3
Takata, M.4
-
36
-
-
69449098184
-
Mobilityshiftdetection ofphosphorylation on large proteins using a Phos-tag SDS-PAGE gelstrengthened with agarose
-
Kinoshita, E., Kinoshita-Kikuta, E., Ujihara, H. & Koike, T. Mobilityshiftdetection ofphosphorylation on large proteins using a Phos-tag SDS-PAGE gelstrengthened with agarose. Proteomics, in press (2009).
-
(2009)
Proteomics
-
-
Kinoshita, E.1
Kinoshita-Kikuta, E.2
Ujihara, H.3
Koike, T.4
-
37
-
-
34047133301
-
Disease proteomics of high-molecular-mass proteins by two-dimensional gel electrophoresis with agarose gels in the first dimension (Agarose 2-DE)
-
Oh-Ishi, M. & Maeda, T. Disease proteomics of high-molecular-mass proteins by two-dimensional gel electrophoresis with agarose gels in the first dimension (Agarose 2-DE). J. Chromatogr. B 849, 211-222 (2007).
-
(2007)
J. Chromatogr. B
, vol.849
, pp. 211-222
-
-
Oh-Ishi, M.1
Maeda, T.2
-
38
-
-
0028855384
-
Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose
-
Tatsumi, R. & Hattori, A. Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose. Anal. Biochem. 224, 28-31 (1995).
-
(1995)
Anal. Biochem
, vol.224
, pp. 28-31
-
-
Tatsumi, R.1
Hattori, A.2
-
39
-
-
0038219368
-
Vertical agarose gel electrophoresis and electroblotting of high-molecular-weight proteins
-
Warren, C.M., Krzesinski, P.R. & Greaser, M.L. Vertical agarose gel electrophoresis and electroblotting of high-molecular-weight proteins. Electrophoresis 24, 1695-1702 (2003).
-
(2003)
Electrophoresis
, vol.24
, pp. 1695-1702
-
-
Warren, C.M.1
Krzesinski, P.R.2
Greaser, M.L.3
-
40
-
-
33846337438
-
Single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gelelectrophoresis. Anal
-
Kinoshita, E., Kinoshita-Kikuta, E. & Koike, T. A single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gelelectrophoresis. Anal. Biochem. 361, 294-298 (2007).
-
(2007)
Biochem
, vol.361
, pp. 294-298
-
-
Kinoshita, E.1
Kinoshita-Kikuta, E.2
Koike, T.A.3
-
41
-
-
43849094336
-
Mobility shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamide gel electrophoresis
-
Kinoshita-Kikuta, E., Kinoshita, E. & Koike, T. A mobility shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamide gel electrophoresis. Anal. Biochem. 378, 102-104 (2008).
-
(2008)
Anal. Biochem
, vol.378
, pp. 102-104
-
-
Kinoshita-Kikuta, E.1
Kinoshita, E.2
Koike, T.A.3
-
42
-
-
0028360374
-
A mammalian protein targeted by G1-arresting rapamycin- receptor complex
-
Brown, E.J. et al. A mammalian protein targeted by G1-arresting rapamycin- receptor complex. Nature 369, 756-758 (1994).
-
(1994)
Nature
, vol.369
, pp. 756-758
-
-
Brown, E.J.1
-
43
-
-
0028950217
-
Isolation ofa protein target ofthe FKBP12-rapamycin complex in mammalian cells
-
Sabers, C.J. etal. Isolation ofa protein target ofthe FKBP12-rapamycin complex in mammalian cells. J. Biol. Chem. 270, 815-822 (1995).
-
(1995)
J. Biol. Chem
, vol.270
, pp. 815-822
-
-
Sabers, C.J.1
-
44
-
-
0035798097
-
Mammalian TOR: A homeostatic ATP sensor
-
Dennis, P.B. et al. Mammalian TOR: a homeostatic ATP sensor. Science 294, 1102-1105 (2001).
-
(2001)
Science
, vol.294
, pp. 1102-1105
-
-
Dennis, P.B.1
-
45
-
-
0035312747
-
Regulation of translation initiation by FRAP/mTOR
-
Gingras, A.C., Raught, B. & Sonenberg, N. Regulation of translation initiation by FRAP/mTOR. Genes Dev. 15, 807-826 (2001).
-
(2001)
Genes Dev
, vol.15
, pp. 807-826
-
-
Gingras, A.C.1
Raught, B.2
Sonenberg, N.3
-
46
-
-
0033429554
-
Mammalian target of rapamycin is a direct target for protein kinase B: Identication ofa convergence point for opposing effects ofinsulin and amino-acid deficiency on protein translation
-
Nave, B.T. et al. Mammalian target of rapamycin is a direct target for protein kinase B: identication ofa convergence point for opposing effects ofinsulin and amino-acid deficiency on protein translation. Biochem. J. 344, 427-431 (1999).
-
(1999)
Biochem. J
, vol.344
, pp. 427-431
-
-
Nave, B.T.1
-
47
-
-
0034629365
-
FKBP12-rapamycin- associated protein (FRAP) autophosphorylates at serine 2481 under translationally repressive conditions
-
Peterson, R.T., Beal, P.A., Comb, M.J. & Schreiber, S.L. FKBP12-rapamycin- associated protein (FRAP) autophosphorylates at serine 2481 under translationally repressive conditions. J. Biol. Chem. 275, 7416-7423 (2000).
-
(2000)
J. Biol. Chem
, vol.275
, pp. 7416-7423
-
-
Peterson, R.T.1
Beal, P.A.2
Comb, M.J.3
Schreiber, S.L.4
-
48
-
-
0037472924
-
DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation
-
Bakkenist, C.J. & Kastan, M.B. DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature 421, 499-506 (2003).
-
(2003)
Nature
, vol.421
, pp. 499-506
-
-
Bakkenist, C.J.1
Kastan, M.B.2
-
49
-
-
0035972194
-
Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways
-
Rappold, I., Iwabuchi, K., Date, T. & Chen, J. Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways. J. Cell Biol. 153, 613-620 (2001).
-
(2001)
J. Cell Biol
, vol.153
, pp. 613-620
-
-
Rappold, I.1
Iwabuchi, K.2
Date, T.3
Chen, J.4
-
50
-
-
0038143321
-
Accumulation ofcheckpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX
-
Ward, I.M., Minn, K., Jorda, K.G. & Chen, J. Accumulation ofcheckpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX. J. Biol. Chem. 278, 19579-19582 (2003).
-
(2003)
J. Biol. Chem
, vol.278
, pp. 19579-19582
-
-
Ward, I.M.1
Minn, K.2
Jorda, K.G.3
Chen, J.4
|