Mobility shift detection of phosphorylation on large proteins using a Phos-tag SDS-PAGE gel strengthened with agarose

Proteomics

Volumn 9, Issue 16, 2009, Pages 4098-4101

  Kinoshita, Eiji ^a   Kinoshita Kikuta, Emiko ^a   Ujihara, Hiromi ^a   Koike, Tohru ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

High molecular mass proteins; Phos tag; Phosphate affinity SDS PAGE; Phosphorylation; Technology; Western blotting

Indexed keywords

AGAROSE; METAL ION; PHOS TAG; PHOSPHATE BINDING PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; GEL MOBILITY SHIFT ASSAY; IMMUNOBLOTTING; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION;

BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR WEIGHT; PHOSPHORYLATION; PROTEINS; SEPHAROSE;

PHOS;

EID: 69449098184     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.200900020     Document Type: Article

References (8)

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