Characterization of cyclo-acetoacetyl-l-tryptophan dimethylallyltransferase in cyclopiazonic acid biosynthesis: substrate promiscuity and site directed mutagenesis studies

Biochemistry

Volumn 48, Issue 46, 2009, Pages 11032-11044

  Liu, Xinyu ^a   Walsh, Christopher T ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPERGILLUS SP; COMPARATIVE EVALUATIONS; CYCLOPIAZONIC ACID; DIKETOPIPERAZINES; L-TRYPTOPHAN; LINEAR PEPTIDES; N-TERMINALS; NANOMOLAR INHIBITORS; NONRIBOSOMAL PEPTIDE SYNTHETASES; POLYKETIDE SYNTHASES; PRENYLATION; REGIOSPECIFIC; SEQUENCE ALIGNMENTS; SITE DIRECTED MUTAGENESIS; TETRAMIC ACIDS; TYROSINE RESIDUES;

AMINES; BIOCHEMISTRY; CALCIUM; ENZYMES; MUTAGENESIS; ORGANIC ACIDS; PEPTIDES; POLYPEPTIDES; SUBSTRATES;

AMINO ACIDS;

CYCLOPIAZONIC ACID; DIMETHYLALLYLTRANSFERASE; PEPTIDE DERIVATIVE; PIPERAZINEDIONE; TETRAMIC ACID DERIVATIVE; THIOHYDANTOIN DERIVATIVE;

ARTICLE; ASPERGILLUS; ASPERGILLUS FLAVUS; ASPERGILLUS ORYZAE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SUBSTRATE; NONHUMAN; PRIORITY JOURNAL; PROTEIN MOTIF; SITE DIRECTED MUTAGENESIS;

ALKYL AND ARYL TRANSFERASES; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ASPERGILLUS FLAVUS; ASPERGILLUS ORYZAE; BIOCATALYSIS; CATIONS, DIVALENT; CONSERVED SEQUENCE; DIKETOPIPERAZINES; DIPEPTIDES; INDOLES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POLYISOPRENYL PHOSPHATES; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, TERTIARY; PYRROLIDINONES; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; THIOHYDANTOINS; TRYPTOPHAN;

ASPERGILLUS SP.;

EID: 72749089599     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi901597j     Document Type: Article

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