Separation by hydrophobic interaction chromatography and structural determination by mass spectrometry of mannosylated glycoforms of a recombinant transferrin-exendin-4 fusion protein from yeast

Journal of Chromatography A

Volumn 1217, Issue 2, 2010, Pages 225-234

  Zolodz, Melissa D ^a   Herberg, John T ^a   Narepekha, Halyna E ^a   Raleigh, Emily ^a   Farber, Matthew R ^a   Dufield, Robert L ^a   Boyle, Denis M ^a  

^a PFIZER INC   (United States)

Author keywords

ETD; Hydrophobic interaction chromatography (HIC); Mannosylation; Mass spectrometry; NPLC MS; Transferrin; Yeast

Indexed keywords

BIOTECHNOLOGY INDUSTRY; CARBOHYDRATE CONTENT; ELECTRON TRANSFER DISSOCIATION; ETD; FUSION PROTEINS; GLYCANS; GLYCOFORMS; GLYCOPEPTIDES; GLYCOSIDIC LINKAGES; GLYCOSYLATED; HIGH DENSITY FERMENTATIONS; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; HYDROPHOBIC INTERACTION CHROMATOGRAPHY (HIC); LINKER PEPTIDE; MANNOSE SUGARS; N-TERMINALS; NEW HIGH; POTENTIAL SITES; RECOMBINANT PROTEIN; STRUCTURAL DETERMINATION; TECHNOLOGICAL CAPABILITY; THREONINE RESIDUES; TRYPTIC PEPTIDES; YEAST SACCHAROMYCES CEREVISIAE;

AMINATION; AMINES; AMINO ACIDS; BIOLOGY; CHAIN LENGTH; CHROMATOGRAPHIC ANALYSIS; DISSOCIATION; HYDROPHOBICITY; MASS SPECTROMETERS; MASS SPECTROMETRY; ORGANIC ACIDS; PEPTIDES; SUGAR (SUCROSE); SUGARS; YEAST;

HYDROPHOBIC CHROMATOGRAPHY;

EXENDIN 4; EXENDIN 4 TRANSFERRIN FUSION PROTEIN; GLYCAN; HYBRID PROTEIN; ISOPROTEIN; MANNOSE; SERINE; THREONINE; TRANSFERRIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; ELECTRON TRANSPORT; FERMENTATION; HUMAN; HYDROPHOBICITY; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN PROCESSING; PROTEIN STRUCTURE; PROTEIN VARIANT; SACCHAROMYCES CEREVISIAE; YEAST;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, LIQUID; GLYCOPEPTIDES; HUMANS; HYDROPHOBICITY; MANNOSE; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PEPTIDES; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; TANDEM MASS SPECTROMETRY; TRANSFERRIN; UREA; VENOMS;

SACCHAROMYCES CEREVISIAE;

EID: 72449154747     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2009.10.057     Document Type: Article

References (57)

1. Melander W., and Horvath C. Arch. Biochem. Biophys. 183 (1977) 200
2. Strahl-Bolsinger S., Gentzsch M., and Tanner W. Biochim. Biophys. Acta 1426 (1999) 297
3. In: Sofer G., and Hagel L. (Eds). Handbook of Process Chromatography: A Guide to Optimization, Scale-up and Validation (1998), Academic Press, San Diego 387
4. Mahn A., Lienqueo M.E., and Asenjo J.A. J. Chromatogr. B 849 (2007) 236
5. Jennissen H.P. Int. J. Bio-Chromatogr. 5 (2000) 131
6. To B.C.S., and Lenhoff A.M. J. Chromatogr. A 1141 (2007) 191
7. Fausnaugh J.L., Kennedy L.A., and Regnier F.E. J. Chromatogr. 317 (1984) 141
8. Arakawa T., and Narhi L.O. Biotechnol. Appl. Biochem. 13 (1991) 151
9. Timasheff S.N., and Inoue H. Biochemistry 7 (1968) 2501
10. Lin F.Y., Chen W.Y., and Hearn M.T.W. Anal. Chem. 73 (2001) 3875
11. Haimer E., Tscheliessnig A., Hahn R., and Jungbauer A. J. Chromatogr. A 1139 (2007) 84
12. Perkins T.W., Mak D.S., Root T.W., and Lightfoot E.N. J. Chromatogr. A 766 (1997) 1
13. Jones T.T., and Fernandez E.J. Biotechnol. Bioeng. 87 (2004) 388
14. Jungbauer A., Machold C., and Hahn R. J. Chromatogr. A 1079 (2005) 221
15. Swartz J.R. Curr. Opin. Biotechnol. 12 (2001) 195
16. Barnes L., and Dickson A.J. Curr. Opin. Biotechnol. 17 (2006) 381
17. Barnes C.A.S., and Lim A. Mass Spectrom. Rev. 26 (2007) 370
18. Bogosian G., Violand B.N., Dorward-King E.J., Workman W.E., Jung P.E., and Kane J.F. J. Biol. Chem. 264 (1989) 531
19. Baneyx F. Curr. Opin. Biotechnol. 10 (1999) 411
20. Eng M., Ling V., Briggs J.A., Souza K., Canova-Davis E., Powell M.F., and Young L.R.D. Anal. Chem. 69 (1997) 4184
21. Yuk I.H.Y., and Wang D.I.C. Biotechnol. Appl. Biochem. 36 (2002) 133
22. Gemmill T.R., and Trimble R.B. Biochem. Biophys. Acta 1426 (1999) 227
23. Cordoba-Rodriguez R.V. BioPharm Int. 21 (2008) 44
24. van Veen H.A., Geerts M.E., van Berkel P.H., and Nuijens J.H. Eur. J. Biochem. 271 (2004) 678
25. Valmu L., Kalkinnen N., Husa A., and Rye P.D. Biochemistry 44 (2005) 16007
26. S.V. Kaveri, S. Lacroix-Desmzes, J. Bayry, S. Dasgupta, A. Chtourou, in: WPO (Ed.), France, 2007.
27. Storring P.L. Trends Biotechnol. 10 (1992) 427
28. Hermeling S., Crommelin D.J.A., Schellekens H., and Jiskoot W. Pharm. Res. 21 (2004) 897
29. Mukovozov I., Sabljic T., Hortelano G., and Ofosu F.A. Thromb. Haemost. 99 (2008) 874
30. Imperiali M., Thoma C., Pavoni E., Brancaccio A., Callewaert N., and Oxenius O. J. Virol. 79 (2005) 14297
31. Wasley L.C., Timony G., Murtha P., Stoudemire J., Dorner A.J., Caro J., Krieger M., and Kaufman R.J. Blood 77 (1991) 2624
32. Varki A. In: Varki A., Cummings R., Esko J., Freeze H., Hart G., and Marth J. (Eds). Essentials of Glycobiology (1999), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 57
33. Walsh G. Nat. Biotechnol. 24 (2006) 769
34. Pichuantes S., Nguyen A.T., and Franzusoff A. In: Cleland J.L., and Craik C.S. (Eds). Protein Engineering: Principles and Practice (1996), John Wiley & Sons, New York 129
35. Bewley M.C., Tam B.M., Grewal J., He S., Shewry S., Murphy M.E., Mason A.B., Woodworth R.C., Baker E.N., and MacGillivray R.T.A. Biochemistry 38 (1999) 2535
36. Zhang H., Li X.-J., Wang D.-J., Chen J., Li Y.-L., Shen M.-S., Fang H.-Q., and Chen H.-P. Chin. J. Biotechnol. 21 (2005) 804
37. Steinlein L.M., Graf T.N., and Ikeda R.A. Protein Expr. Purif. 6 (1995) 619
38. Sargent P.J., Farnaud S., Cammack R., Zoller H.M.P., and Evans R.W. Biometals 19 (2006) 513
39. Lommel M., and Strahl S. Glycobiology 19 (2009) 816
40. Coon J.J. Anal. Chem. 81 (2009) 3208
41. Steen H., and Mann M. Nat. Rev. Mol. Cell Biol. 5 (2004) 699
42. Wiesner J., Premsler T., and Sickmann A. Proteomics 8 (2008) 4466
43. Syka J.E.P., Coon J.J., Schroeder M.J., Shabanawitz J., and Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 9528
44. Zolodz M., Dufield R., Carroll J., and Mozier N. CA Separation Sciences Society Mass Spectrometry (2008), Napa, CA
45. Doyle M.E., and Egan J.M. Pharmacol. Ther. 113 (2007) 546
46. Krutchinsky A.N., Chernushevich I.V., Spicer V.L., Ens W., and Standing K.G. J. Am. Soc. Mass Spectrom. 9 (1998) 569
47. Huang Y., Mechref Y., and Novotny M.V. Anal. Chem. 73 (2001) 6063
48. Herberg J.T., Raleigh E., and Boyle D.M. 6th HIC-RP Bioseparation Conference. Napa, CA (2009)
49. Bousfield G.R., Butnev V.Y., Bidart J.-M., Dalpathado D., Irungu J., and Desaire H. Biochemistry 47 (2008) 1708
50. Arndt T., and Kropf J. Clin. Chem. 48 (2002) 2072
51. De la Calle Guntinas M.B., Bordin G., and Rodriguez A.R. Anal. Bioanal. Chem. 378 (2004) 383
52. Olah S., Kremmer T., and Boldizsar M. J. Chromatogr. B: Biomed. Sci. Appl. 744 (2000) 73
53. Mason A.B., Halbrooks P.J., Larouche J.R., Briggs S.K., Moffett M.L., Ramsey J.E., Connolly S.A., Smith V.C., and MacGillivray R.T.A. Protein Expr. Purif. 36 (2004) 318
54. Fenaille F., Le Mignon M., Groseil C., Siret L., and Bihoreau N. Rapid Commun. Mass Spectrom. 21 (2007) 812
55. Guile G.R., Rudd P.M., Wing D.R., Prime S.B., and Dwek R.A. Anal. Biochem. 240 (1996) 210
56. Gesquiere J.C., Diesis E., Cung M.T., and Tartar A. J. Chromatogr. 478 (1989) 121
57. Cech N.B., and Enke C.G. Mass Spectrom. Rev. 20 (2001) 362