Chromatofocusing fails to separate hFSH isoforms on the basis of glycan structure

Biochemistry

Volumn 47, Issue 6, 2008, Pages 1708-1720

  Bousfield, George R ^a   Butnev, Vladimir Y ^a   Bidart, Jean Michel ^b   Dalpathado, Dilusha ^c   Irungu, Janet ^c   Desaire, Heather ^c  

^a WICHITA STATE UNIVERSITY   (United States)

^b INSTITUT GUSTAVE ROUSSY   (France)

^c UNIVERSITY OF KANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOACTIVITY; GLYCOSYLATION; IMMUNOLOGY;

GLYCAN STRUCTURE; PHYSIOLOGICAL FLUIDS; PITUITARY GLAND EXTRACTS;

HORMONES;

ASPARAGINE; FOLLITROPIN; GLYCAN; GLYCOPEPTIDASE; GLYCOPEPTIDE; ISOPROTEIN; SIALIC ACID;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CHROMATOFOCUSING; CONTROLLED STUDY; GLYCOSYLATION; GONAD; HORMONE DETERMINATION; HORMONE STRUCTURE; HYPOPHYSIS; INHIBITION KINETICS; ISOELECTRIC POINT; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; RECEPTOR BINDING; URINE; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CARBOHYDRATE CONFORMATION; CHO CELLS; CRICETINAE; CRICETULUS; FOLLICLE STIMULATING HORMONE; HUMANS; MASS SPECTROMETRY; POLYSACCHARIDES; PROTEIN ISOFORMS; RADIOIMMUNOASSAY; RATS;

TETRA;

EID: 38949180281     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi701764w     Document Type: Article

References (37)

1. Bousfield, G. R., Jia, L., and Ward, D. N. (2006) Gonadotropins: Chemistry and biosynthesis, in Knobil and Neill: Physiology of Reproduction (Neill, J. D., Ed.) pp 1581-1634, Elsevier, San Diego.
2. Hearn, M. T. W., and Gomme, P. T. (2000) Molecular architecture and biorecognition processes of the cystine knot protein superfamily: Part I. The glycoprotein hormones, J. Mol. Recognit. 13, 223-278.
3. 56 oligosaccharide structure on equine Lutropin and Follitropin hybrid conformation and receptor-binding activity, Biochemistry 43, 10817-10833.
4. 1H NMR spectroscopy, Glycobiology 1, 393-404.
5. Liu, C., and Bowers, L. D. (1997) Mass spectrometric characterization of nicked fragments of the β-subunit of human chorionic gonadotropin, Clin. Chem. 43, 1172-1181.
6. Valmu, L., Alfthan, H., Hotakainen, K., Birken, S., and Stenman, U. H. (2006) Site-specific glycan analysis of human chorionic gonadotropin β-subunit from malignancies and pregnancy by liquid chromatography- electrospray mass spectrometry, Glycobiology 16, 1207-1218.
7. Ulloa-Aguirre, A., Midgley, A. R., Jr., Beitins, I. Z., and Padmanbhan, V. (1995) Follicle-stimulating isohormones: Characterization and physiological relevance, Endocr. Rev. 16, 765-787.
8. Stanton, P. G., Robertson, D. M., Burgon, P. G., Schmauk-White, B., and Hearn, M. T. W. (1992) Isolation and physicochemical characterization of human follicle-stimulating hormone isoforms, Endocrinology 130, 2820-2832.
9. Stanton, P. G., Shen, Z., Kecorius, E. A., Bergon, P. G., Roberson, D. M., and Hearn, M. T. (1995) Application of a sensitive HPLC-based fluorometric assay to determine the sialic acid content of human gonadotropin isoforms, J. Biochem. Biophys. Methods 30, 37-48.
10. Stanton, P. G., Burgon, P. G., Hearn, M. T. W., and Robertson, D. M. (1996) Structural and functional characterization of hFSH and hLH isoforms, Mol. Cell. Endocrinol. 125, 133-141.
11. Walton, W. J., Nguyen, V. T., Butnev, V. Y., Singh, V., Moore, W. T., and Bousfield, G. R. (2001) Characterization of human follicle-stimulating hormone isoforms reveals a non-glycosylated β-subunit in addition to the conventional glycosylated β-subunit, J. Clin. Endorinol. Metab. 86, 3675-3685.
12. Green, E. D., and Baenziger, J. U. (1988) Asparagine-linked oligosaccharides on lutropin, follitropin, and thyrotropin: I. Structural elucidation of the sulfated and sialylated oligosaccharides on bovine, ovine and human pituitary glycoprotein hormones, J. Biol. Chem. 263, 25-35.
13. Green, E. D., and Baenziger, J. U. (1988) Asparagine-linked oligosaccharides on lutropin, follitropin, and thyrotropin II. distributions of sulfated and sialylated oligosaccharides on bovine, ovine, and human pituitary glycoprotein hormones, J. Biol. Chem. 263, 36-44.
14. Renwick, A. G. C., Mizuochi, T., Kochibe, N., and Kobata, A. (1987) The asparagine-linked sugar chains of human follicle-stimulating hormone, J. Biochem. 101, 1209-1221.
15. Dalpathado, D. S., Irungu, J. A., Go, E. P., Butnev, V. Y., Norton, K., Bousfield, G. R., and Desaire, H. (2006) Comparative glycomics of the glycoprotein hormone follicle-stimulating hormone (FSH): Glycopeptide analysis of isolates from two mammalian species, Biochemistry 45, 8665-8673.
16. Jiang, H., Butnev, V. Y., Bousfield, G. R., and Desaire, H. (2004) Glycoprotein profiling by electrospray mass spectrometry, J. Am. Soc. Mass Spectrom. 15, 750-758.
17. Irungu, J. A., Dalpathado, D. S., Go, E. P., Jiang, H., Ha, H.-V., Bousfield, G. R., and Desaire, H. (2006) A Method for Characterizing Sulfated Glycoproteins in a Glycosylation Site-Specific Fashion, Using Ion-Pairing and Tandem Mass Spectrometry, Anal. Chem. 78, 1181-1190.
18. Calvo, F. O., Keutmann, H. T., Bergert, E. R., and Ryan, R. J. (1986) Deglycosylated human follitropin: Characterization and effects on adenosine cyclic 3′,5′-phosphate production in porcine granulosa cells, Biochemistry 25, 3938-3943.
19. Gordon, W. L., Bousfield, G. R., and Ward, D. N. (1989) Comparative binding of FSH to chicken and rat testis, J. Endocrinol. Invest. 12, 383-392.
20. Bidart, J.-M., Troalen, F., Lazar, V., Berger, P., Marcillac, I., Lhomme, C., Droz, J.-P., and Bellet, D. (1992) Monoclonal antibodies to the free β-subunit of human chorionic gonadotropin define three distinct antigenic domains and distinguish between intact and nicked molecules, Endocrinology 131, 1832-1840.
21. 56 oligosaccharide on in vitro subunit association and FSH receptor binding of equine gonadotropins, Biol. Reprod. 58, 458-469.
22. 56, Endocrinology 137, 2543-2557.
23. Bousfield, G. R., Butnev, V. Y., Walton, W. J., Nguyen, V. T., Singh, V., Hueneidi, J., Kolli, K., Harvey, D. J., and Rance, N. (2007) All or none N-glycosylation in primate follicle-stimulating hormone subunits, Mol. Cell. Endocrinol. 260-262, 40-48.
24. Bousfield, G. R., Baker, V. L., Gotschall, R. R., Butnev, V. Y., and Butnev, V. Y. (2000) Carbohydrate analysis of glycoprotein hormones, Methods 21, 15-39.
25. Johnston, R. C., Stanton, P. G., Robertson, D. M., and Hearn, M. T. (1987) High-performance liquid chromatography of amino acids, peptides and proteins. LXXXV. Separation of isoforms of the glycoprotein hormones from human pituitary extracts, J. Chromatogr. 397, 389-398.
26. Creus, S., Chaia, Z., Pellizzari, E. H., Cigorraga, S. B., Ulloa-Aguirre, A., and Campo, S. (2001) Human FSH isoforms: Carbohydrate complexity as determinant of in-vitro bioactivity, Mol. Cell. Endocrinol. 174, 41-49.
27. Timossi, C. M., Barrios de Tomasi, J., Zambrano, E., Gonzalez, R., and Ulloa-Aguirre, A. (1998) A naturally occurring basically charge human follicle-stimulating hormone (FSH) variant inhibits FSH-induced androgen aromatization and tissue-type plasminogen activator enzyme activity in vitro, Neuroendocrinology 67, 153-163.
28. Fan, Q. R., and Hendrickson, W. A. (2005) Structure of human follicle-stimulating hormone in complex with its receptor, Nature 433, 269-277.
29. Bousfield, G. R., Butnev, V. Y., Gotschall, R. R., Baker, V. L., and Moore, W. T. (1996) Structural features of mammalian gonadotropins, Mol. Cell. Endocrinol. 125, 3-19.
30. Bishop, L. A., Robertson, D. M., Cahir, N., and Schofield, P. R. (1994) Specific roles for the asparagine-linked carbohydrate residues of recombinant human follicle stimulating hormone in receptor binding and signal transduction, J. Mol. Endocrinol. 8, 722-731.
31. Flack, M. R., Froehlich, J., Bennet, A. P., Anasti, J., and Nisula, B. C. (1994) Site-directed mutagenesis defines the individual roles of the glycosylation sites on follicle-stimulating hormone, J. Biol. Chem. 269, 14015-14020.
32. Townsend, R. R., and Hardy, M. R. (1991) Analysis of glycoprotein oligosaccharides using high-pH anion exchange chromatography, Glycobiology 1, 139-147.
33. Couture, L., Remy, J. J., Rabesona, H., Troalen, F., Pajot-Augy, E., Bozon, V., Haertle, T., Bidart, J. M., and Salesse, R. (1996) A defined epitope on the human choriogonadotropin α-subunit interacts with the second extracellular loop of the transmembrane domain of the lutropin/ choriogonadotropin receptor, Eur. J. Biochem. 241, 627-632.
34. Robert, P. (1995) in Pharmaceutical Sciences, pp 101, Université René Descartes de Paris, Paris.
35. Bousfield, G. R., and Ward, D. N. (1994) Evidence for two folding domains in glycoprotein hormone α subunits, Endocrinology 135, 624-635.
36. Bousfield, G. R., Liu, W.-K., and Ward, D. N. (1985) Hybrids from equine LH: α enhances, β diminishes activity, Mol. Cell. Endocrinol. 40, 69-77.
37. Louriero, R. F., de Oliveira, J. E., Torjesen, P. A., Bartolini, P., and Ribela, M. T. C. P. (2006) Analysis of intact human follicle-stimulating hormone preparations by reversed-phase high-performance liquid chromatography, J. Chromatogr., A 1136, 10-18.