Cloning and functional characterisation of a fatty acyl elongase from southern bluefin tuna (Thunnus maccoyii)

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Volumn 155, Issue 2, 2010, Pages 178-185

  Gregory, Melissa K ^a   See, Valene H L ^a   Gibson, Robert A ^b   Schuller, Kathryn A ^a  

^a FLINDERS UNIVERSITY   (Australia)

^b UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

Aquaculture; cDNA; Fatty acyl elongase; Long chain polyunsaturated fatty acids; Southern bluefin tuna; Yeast expression

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; DOCOSAHEXAENOIC ACID; ENZYME; FATTY ACYL ELONGASE; ICOSAPENTAENOIC ACID; MONOUNSATURATED FATTY ACID; OMEGA 3 FATTY ACID; POLYUNSATURATED FATTY ACID; SATURATED FATTY ACID; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; ENZYME ANALYSIS; FATTY ACID ANALYSIS; FATTY ACID SYNTHESIS; GENE AMPLIFICATION; GENE EXPRESSION; GENE SEQUENCE; GENETIC CONSERVATION; LIVER; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHYLOGENY; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; TUNA;

SACCHAROMYCES CEREVISIAE; THUNNUS MACCOYII;

EID: 71849091908     PISSN: 10964959     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpb.2009.11.002     Document Type: Article

References (29)

1. Agaba M., Tocher D.R., Dickson C.A., Dick J.R., and Teale A.J. Zebrafish cDNA encoding multifunctional fatty acid elongase involved in production of eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Mar. Biotechnol. 6 (2004) 251-261
2. Agaba M.K., Tocher D.R., Zheng X., Dickson C.A., Dick J.R., and Teale A.J. Cloning and functional characterisation of polyunsaturated fatty acid elongases of marine and freshwater teleosts fish. Comp. Biochem. Physiol. B 142 (2005) 342-352
3. Alimuddin, Kiron V., Satoh S., Takeuchi T., and Yoshizaki G. Cloning and over-expression of a masu salmon (Oncorhynchus masou) fatty acid elongase-like gene in zebrafish. Aquaculture 282 (2008) 13-18
4. Beaudoin F., Michaelson L.V., Hey S.J., Lewis M.J., Shewry P.R., Sayanova O., and Napier J.A. Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthesis pathway. Proc. Natl. Acad. Sci. USA 97 (2000) 6421-6426
5. Hastings N., Agaba M.K., Tocher D.R., Zheng X., Dickson C.A., Dick J.R., and Teale A.J. Molecular cloning and functional characterization of fatty acyl desaturase and elongase cDNAs involved in the production of eicosapentaenoic acid and docosahexaenoic acids from α-linolenic acid in Atlantic salmon (Salmo salar). Mar. Biotechnol. 6 (2005) 463-474
6. Inagaki K., Aki T., Fukuda Y., Kawamoto S., Shigeta S., Ono K., and Suzuki O. Identification and expression of a rat fatty acid elongase involved in the biosynthesis of C18 fatty acids. Biosci. Biotechnol. Biochem. 66 3 (2002) 613-621
7. Jakobsson A., Westerberg R., and Jacobsson A. Fatty acid elongases in mammals: their regulation and roles in metabolism. Prog. Lipid Res. 45 (2006) 237-249
8. Jenkins D.J.A., Sievenpiper J.L., Pauly D., Sumaila U.R., Kendell C.W.C., and Mowat F.M. Are dietary recommendations for the use of fish oils sustainable?. CMAJ 180 6 (2009) 633-637
9. Leonard A.E., Bobik E.G., Dorado J., Kroeger P.E., Chuang L., Thurmond J.M., Parker-Barnes J.M., Das T., Huang Y., and Mukerji P. Cloning of a human cDNA encoding a novel enzyme involved in the elongation of long-chain polyunsaturated fatty acids. Biochem. J. 350 (2000) 765-770
10. Leonard A.E., Kelder B., Bobik E.G., Chuang L., Lewis C.J., Kopchick J.J., Mukerji P., and Huang Y. Identification and expression of mammalian long-chain PUFA elongation enzymes. Lipids 37 8 (2002) 733-740
11. Metcalf R.G., Sanders P., James M.J., Cleland L.G., and Young G.D. Effect of dietary n-3 polyunsaturated fatty acids on the inducibility of ventricular tachycardia in patients with ischemic cardiomyopathy. Am. J. Cardiol. 101 6 (2008) 758-761
12. Meyer A., Kirsch H., Domergue F., Abbadi A., Sperling P., Bauer J., Cirpus P., Zank T.K., Moreau H., Roscoe T.J., Zahringer U., and Heinz E. Novel fatty acid elongases and their use for the reconstitution of docosahexaenoic acid biosynthesis. J. Lipid Res. 45 (2004) 1899-1909
13. Morais S., Monroig O., Zheng X., Leaver M.J., and Tocher D.R. Highly unsaturated fatty acid synthesis in Atlantic salmon: characterization of ELOVL5- and ELOVL2-like elongases. Mar. Biotechnol. 11 5 (2009) 627-639
14. Mourente G., Megina C., and Diaz-Salvago E. Lipids in female northern bluefin tuna (Thunnus thynnus thynnus L.) during sexual maturation. Fish Physiol. Biochem. 24 (2002) 351-363
15. Mourente G., and Tocher D.R. An approach to study the nutritional requirements of the bluefin tuna (Thunnus thynnus thynnus L.). In: Bridges C.R., Gordin H., and Garcia A. (Eds). Proceedings of the International Symposium on Domestication of the Bluefin Tuna, Thunnus thynnus thynnus, 3-8 February 2002. Cartagena, Spain. Cah. Options Mediterr. vol. 60 (2003) 143-150
16. Naylor R.L., Goldburg R.J., Primavera J.H., Kautsky N., Beveridge M.C.M., Clay J., Folke C., Lubchenco J., Mooney H., and Troell M. Effect of aquaculture on world fish supplies. Nature 405 (2000) 1017-1024
17. Nichols P.D., Virtue P., Mooney B.D., Elliott N.G., and Yearsley G.K. Seafood the good food: the oil (fat) content and composition of Australian commercial fishes, shellfishes and crustaceans. FRDC Project 95/122 (1998)
18. Park W.J., Kothapalli K.S.D., Lawrence P., Tyburczy C., and Brenna J.T. An alternate pathway to long chain polyunsaturates: the FADS2 gene product Δ8-desaturates 20:2n-6 And 20:3n-3. J. Lipid Res. 50 (2009) 1195-1202
19. Parker-Barnes J.M., Das T., Bobik E., Leonard A.E., Thurmond J.M., Chaung L., Huang Y., and Mukerji P. Identification and characterization of an enzyme involved in the elongation of n-6 and n-3 polyunsaturated fatty acids. Proc. Natl. Acad. Sci. USA 97 15 (2000) 8284-8289
20. Proudman S.M., Cleland L.G., and James M.J. Dietary omega-3 fats for treatment of inflammatory joint disease: efficacy and utility. Rheum. Dis. Clin. North Am. 34 (2008) 469-479
21. Regost C., Arzel J., Robin J., Rosenlund G., and Kaushik S.J. Total replacement of fish oil by soybean or linseed oil with a return to fish oil in turbot (Psetta maxima) 1. Growth performance, flesh fatty acid profile, and lipid metabolism. Aquaculture 217 (2003) 465-482
22. Saito H., Ishihara K., and Murase T. The fatty acid composition in tuna (bonito, Euthynnus pelamis) caught at three different localities from tropics to temperate. J. Sci. Food Agric. 73 (1997) 53-59
23. Smithers L.G., Gibson R.A., McPhee A., and Makrides M. Effect of long-chain polyunsaturated fatty acid supplementation of preterm infants on disease rick and neurodevelopment: a systematic review of randomized controlled trials. Am. J. Clin. Nutr. 87 (2008) 912-920
24. Sprecher H. Metabolism of highly unsaturated n-3 and n-6 fatty acids. Biochim. Biophys. Acta 1486 (2000) 219-231
25. Swofford D.L. PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4.0b 10 (2003), Sinauer Associates, Sunderland, MA, USA
26. Tocher D.R. Metabolism and functions of lipids and fatty acids in teleost fish. Rev. Fish. Sci. 11 2 (2003) 107-184
27. Tocher D.R., Bell J.G., McGhee F., Dick J.R., and Fonseca-Madrigal J. Effects of dietary lipid level and vegetable oil on fatty acid metabolism in Atlantic salmon (Salmo salar L.) over the whole production cycle. Fish Physiol. Biochem. 29 (2003) 193-209
28. Whelan J., and Rust C. Innovative dietary sources of n-3 fatty acids. Annu. Rev. Nutr. 26 (2006) 75-103
29. Zheng X., Ding Z., Xu Y., Monroig O., Morais S., and Tocher D.R. Physiological roles of fatty acyl desaturases and elongases in marine fish: characterisation of cDNAs of fatty acyl Δ6 desaturase and elovl5 elongase of cobia (Rachycentron canadum). Aquaculture 290 (2009) 122-131