Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel internal amplification control approach using Biotrove OpenArrays

Applied and Environmental Microbiology

Volumn 75, Issue 22, 2009, Pages 7253-7260

  Van Doorn, R ^a,b   Klerks, M M ^a,c   Van Gent Pelzer, M P E ^a   Speksnijder, A G C L ^d   Kowalchuk, G A ^b,e   Schoen, C D ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b NETHERLANDS INSTITUTE OF ECOLOGY NIOO KNAW   (Netherlands)

^c Innosieve Diagnostics BV   (Netherlands)

^d Public Health Laboratory   (Netherlands)

^e VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

AGRICULTURAL ROBOTS; DNA; MICROORGANISMS;

ACCURATE QUANTIFICATIONS; AGRICULTURAL SAMPLES; ENVIRONMENTAL SAMPLE; HIGH THROUGHPUT SCREENING; INTERNAL AMPLIFICATION; PCR-BASED DETECTION; STATE OF THE ART; TARGET CONCENTRATIONS;

POLYMERASE CHAIN REACTION;

BIOASSAY; DETECTION METHOD; DNA; GENE EXPRESSION; INHIBITION; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SOIL MICROORGANISM;

ARTICLE; DNA EXTRACTION; DNA MICROARRAY; GENE TARGETING; HIGH THROUGHPUT SCREENING; MICROORGANISM; NUCLEIC ACID AMPLIFICATION; PH; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SOIL ANALYSIS; SOIL MICROFLORA; SOIL SEED BANK;

EID: 70449358972     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00796-09     Document Type: Article

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