Development of a novel internal positive control for Taqman® based assays

Molecular and Cellular Probes

Volumn 19, Issue 1, 2005, Pages 51-59

  Hartman, Laurie J ^a   Coyne, Susan R ^a   Norwood, David A ^a  

^a Diagnostic Systems Division   (United States)

Author keywords

Exogenous internal positive control; False negative; PCR inhibitors; Taqman PCR

Indexed keywords

EDETIC ACID; FULVIC ACID; HEMOGLOBIN; HEPARIN; HUMIC ACID; NUCLEIC ACID;

ACCURACY; ANALYTIC METHOD; ANALYTICAL ERROR; ARTICLE; BACILLUS ANTHRACIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; MOLECULAR PROBE; NONHUMAN; NUCLEIC ACID ANALYSIS; NUCLEOTIDE SEQUENCE; OBSERVER VARIATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PURIFICATION; SEQUENCE HOMOLOGY;

FALSE NEGATIVE REACTIONS; METHODS; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; REFERENCE STANDARDS; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING;

BACILLUS ANTHRACIS; POSIBACTERIA;

EID: 11844289560     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2004.07.006     Document Type: Article

References (18)

1. R.W. Cone, A.C. Hobson, M.W. Huang, and M.R. Fairfax Polymerase chain reaction decontamination: the wipe test Lancet 336 1990 686 687 [Letter]
2. S. Kwok, and R. Higuchi Avoiding false positives with PCR Nature 339 1989 237 238
3. P.N. Rys, and D.H. Persing Preventing false positives: quantitative evaluation of three protocols for inactivation of polymerase chain reaction amplification products J Clin Microbiol 9 1993 2356 2360
4. J.P. Ursi, D. Ursi, M. Ieven, and S.R. Pattyn Utility of an internal control for the polymerase chain reaction. Application to detection of Mycoplasma pneumoniae in clinical specimens Acta Pathologica, Microbiologica, et Immunologica Scandinavica 100 1992 635 639
5. S. Monpoeho, M. Coste-Burel, M. Costa-Mattioli, B. Besse, J.J. Chomel, and S. Billaudel Application of a real-time polymerase chain reaction with internal positive control for detection and quantification of enterovirus in cerebrospinal fluid Eur J Clin Microbiol Infect Dis 21 2002 532 536
6. M. Gonzague, C. Plin, L. Bakkali-Kassimi, A. Boutrouille, and C. Cruciere Development of an internal control for the detection of the African swine fever virus by PCR Mol Cell Probes 16 2002 237 242
7. L. Nicolas, and C. Plichart A universally applicable internal standard for PCR detection of Wuchereria bancrofti in biological samples Parasite 4 1997 253 257
8. R.W. Cone, A.C. Hobson, and M.W. Huang Coamplified positive control detects inhibition of polymerase chain reactions J Clin Microbiol 30 1992 3185 3189
9. B.C. Courtney, M.M. Smith, and E.A. Henchal Development of internal controls for probe-based nucleic acid diagnostic assays Anal Biochem 270 1999 249 256
10. W.A. Al-Soud, and P. Radstrom Purification and characterization of PCR-inhibitory components in blood cells J Clin Microbiol 39 2001 485 493
11. E. Beutler, T. Gelbart, and W. Kuhl Interference of heparin with the polymerase chain reaction Biotechniques 9 1990 166
12. J. Satsangi, D.P. Jewell, K. Welsh, M. Bunce, and J.I. Bell Effect of heparin on polymerase chain reaction Lancet 343 1994 1509 1510
13. M. Holodniy, S. Kim, D. Katzenstein, M. Konrad, E. Groves, and T.C. Merigan Inhibition of human immunodeficiency virus gene amplification by heparin J Clin Microbiol 29 1991 676 679
14. Y. Tsai, and B.H. Olson Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction Appl Environ Microbiol 58 1992 2292 2295
15. C.A. Kreader Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein Appl Environ Microbiol 62 1996 1102 1106
16. M.M. Ijzerman, D.R. Dahling, and G.S. Fout A method to remove environmental inhibitors prior to the detection of waterborne enteric viruses by reverse transcription-polymerase chain reaction J Virol Meth 63 1997 145 153
17. D. Ursi, M. Ieven, H.P. Van Bever, and H. Goossens Construction of an internal control for the detection of Chlamydia pneumoniae by PCR Mol Cell Probes 12 1998 235 238
18. S.N. Ho, H.D. Hunt, R.M. Horton, J.K. Pullen, and L.R. Pease Site-directed mutagenesis by overlap extension using the polymerase chain reaction Gene 77 1989 51 59