Robust detection and identification of multiple oomycetes and fungi in environmental samples by using a novel cleavable padlock probe-based ligation detection assay

Applied and Environmental Microbiology

Volumn 75, Issue 12, 2009, Pages 4185-4193

  Van Doorn, R ^a,b   Slawiak M ^a,c   Szemes, M ^a,d   Dullemans, A M ^a   Bonants, P ^a   Kowalchuk, G A ^b,e   Schoen, C D ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b NETHERLANDS INSTITUTE OF ECOLOGY NIOO KNAW   (Netherlands)

^c UNIVERSITY OF GDA SK   (Poland)

^d UNIVERSITY OF BRISTOL   (United Kingdom)

^e VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CLEAVAGE SITES; DETECTION ASSAY; DETECTOR PROBES; DNA SAMPLES; DYNAMIC DETECTION RANGE; ENDONUCLEASE; ENVIRONMENTAL MICROBIOLOGY; ENVIRONMENTAL SAMPLE; GENOMIC DNA; LONG OLIGONUCLEOTIDE; MULTIPLEX ASSAYS; NON-TARGET ORGANISM; OOMYCETES; PATHOGENIC MICROORGANISMS; PROBE-BASED; ROBUST DETECTION; SIMULTANEOUS DETECTION; SINGLE COMPOUND; SPECIFIC DETECTION; SPECIFIC TARGET DETECTION; WATER CIRCULATION SYSTEM;

DETECTORS; DNA; FUNGI; GENES; LADDER NETWORKS; LOCKS (FASTENERS); MICROORGANISMS; NUCLEIC ACIDS; OLIGONUCLEOTIDES; TARGETS;

PROBES;

BIOTIN; DETHIOBIOTIN; ENDONUCLEASE; ENDONUCLEASE IV; GENOMIC DNA; OLIGONUCLEOTIDE; UNCLASSIFIED DRUG;

ADAPTATION; BIOASSAY; DETECTION METHOD; DNA; HYBRIDIZATION; IDENTIFICATION METHOD; PATHOGEN; POLYMERASE CHAIN REACTION; PROBE;

ARTICLE; CONTROLLED STUDY; ENVIRONMENTAL FACTOR; FUNGUS; FUNGUS IDENTIFICATION; HORTICULTURE; MICROARRAY ANALYSIS; NONHUMAN; OOMYCETES; SENSITIVITY AND SPECIFICITY; WATER SAMPLING;

ANIMALS; DNA, FUNGAL; ENVIRONMENTAL MICROBIOLOGY; FUNGI; MOLECULAR DIAGNOSTIC TECHNIQUES; NUCLEIC ACID HYBRIDIZATION; OOMYCETES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

FUNGI; OOMYCETES;

EID: 67149137776     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00071-09     Document Type: Article

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