Application of a real-time polymerase chain reaction with internal positive control for detection and quantification of enterovirus in cerebrospinal fluid

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 21, Issue 7, 2002, Pages 532-536

  Monpoeho, S ^a   Coste Burel, M ^a   Costa Mattioli, M ^a   Besse, B ^a   Chomel, J ^b   Billaudel, S ^a   Ferre V ^a  

^a HÔTEL DIEU   (France)

^b Teaching Hospital   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ADOLESCENT; ADULT; ARTICLE; CELL CULTURE; CEREBROSPINAL FLUID; CEREBROSPINAL FLUID EXAMINATION; CHILD; CONTROLLED STUDY; ENTEROVIRUS; EPIDEMIC; HUMAN; INFANT; MENINGITIS; MICROBIOLOGICAL EXAMINATION; NEWBORN; NONHUMAN; PRIORITY JOURNAL; RELIABILITY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SAMPLING; VALIDATION PROCESS; VIRUS DETECTION; VIRUS INFECTION;

ADOLESCENT; ADULT; CHILD; CHILD, PRESCHOOL; DISEASE OUTBREAKS; ENTEROVIRUS; ENTEROVIRUS INFECTIONS; FALSE NEGATIVE REACTIONS; FEMALE; FRANCE; HUMANS; INFANT; INFANT, NEWBORN; MALE; MENINGITIS, VIRAL; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRAL LOAD; VIRUS CULTIVATION;

EID: 0036053184     PISSN: 09349723     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10096-002-0766-5     Document Type: Article

References (25)

1. Aseptic meningitis in infants <2 years of age: Diagnosis and etiology
2. Viral meningitis
3. Role of the virology laboratory in diagnosis and management of patients with central nervous system disease
4. Comparison of use of cerebrospinal fluid, serum, and throat swab specimens in diagnosis of enteroviral acute neurological infection by a rapid RNA detection PCR assay
5. Detection by PCR of enteroviruses in cerebrospinal fluid during a summer outbreak of aseptic meningitis in Switzerland
6. Reverse-transcription polymerase chain reaction detection of the enteroviruses
7. Rapid detection of enterovirus in clinical specimens using PCR and microwell capture hybridization assay
8. Polymerase chain reaction for the laboratory diagnosis of aseptic meningitis and encephalitis
9. Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay
10. A one-step RT-PCR assay using an enzyme-linked detection system for the diagnosis of enterovirus meningitis
11. Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens
12. Quantitative PCR: Theoretical considerations with practical implications
13. Evaluation of the Roche Amplicor enterovirus PCR assay in the diagnosis of enteroviral central nervous system infections
14. Amplicor enterovirus polymerase chain reaction in patients with aseptic meningitis: A sensitive test limited by amplification inhibitors
15. Quantification of enterovirus RNA in sludge samples using single tube real-time RT-PCR
16. Typing of viruses by combinations of antiserum pools: Application to typing of enteroviruses (coxsackie and echo)
17. High-throughput real-time reverse transcription-PCR quantitation of hepatitis C virus RNA
18. One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses
19. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat
20. Enzymatic RNA amplification of the enteroviruses
21. Diagnosis of enteroviral central nervous system infection by polymerase chain reaction during a large community outbreak
22. Persistent meningo-encephalitis caused by echovirus type 11 in a child with hypogammaglobulinemia
23. Persistent enterovirus infection in culture-negative meningoencephalitis: Demonstration by enzymatic RNA amplification
24. Circulation of enteroviruses and persistence of meningitis cases in the winter of 1999-2000
25. Potential cost savings through rapid diagnosis of enteroviral meningitis