Development of a multiple internal control for clinical diagnostic real-time amplification assays

FEMS Immunology and Medical Microbiology

Volumn 48, Issue 2, 2006, Pages 183-191

  Maaroufi, Younes ^a   De Bruyne, Jean Marc ^a   Duchateau, Valerie ^a   Scheen, Robert ^b   Crokaert, Francoise ^a,b  

^a JULES BORDET INSTITUTE   (Belgium)

^b SAINT PIERRE UNIVERSITY HOSPITAL   (Belgium)

Author keywords

False negative PCR results; Multiple internal control; Real time PCR

Indexed keywords

DNA;

ARTICLE; BLOOD SAMPLING; BONE MARROW BIOPSY; CANCER PATIENT; CONTROL SYSTEM; CONTROLLED STUDY; CYTOMEGALOVIRUS; EPSTEIN BARR VIRUS; FALSE NEGATIVE RESULT; GENE AMPLIFICATION; HEPATITIS B VIRUS; HUMAN; HUMAN TISSUE; LUNG LAVAGE; MICROORGANISM DETECTION; MULTIPLE INTERNAL CONTROL; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; PLASMID; POLYOMA VIRUS; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; STATISTICAL ANALYSIS; TOXOPLASMA GONDII; URINE;

ANIMALS; COMPUTER SYSTEMS; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA PRIMERS; DNA, VIRAL; EPSTEIN-BARR VIRUS INFECTIONS; FALSE NEGATIVE REACTIONS; HEPATITIS B; HEPATITIS B VIRUS; HERPESVIRUS 4, HUMAN; HUMANS; JC VIRUS; POLYMERASE CHAIN REACTION; POLYOMAVIRUS INFECTIONS; TOXOPLASMA; TOXOPLASMOSIS;

EID: 33750090058     PISSN: 09288244     EISSN: 1574695X     Source Type: Journal    
DOI: 10.1111/j.1574-695X.2006.00125.x     Document Type: Article

References (36)

1. Abdulmawjood A, Roth S & Bülte M (2002) Two methods for construction of internal amplification controls for the detection of Escherichia coli O157 by polymerase chain reaction. Mol Cell Probes 16: 335-339.
2. Abu Al-Soud W & Rådström P (1998) Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples. Appl Environ Microbiol 64: 3748-3753.
3. Abu Al-Soud W & Rådström P (2001) Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 39: 485-493.
4. Barkham T & Hoorfar J (2004) Internal amplification control for PCR should not be mandatory in the clinical medical environment. J Clin Microbiol 42: 3379-3380.
5. Beck E, Ludwig G, Auerswald EA, Reiss B & Schaller H (1982) Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19: 327-336.
6. Becker K, Roth R & Peters G (1998) Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene. J Clin Microbiol 36: 2548-2553.
7. Biel SS, Held TS, Landt O, Niedrig M, Gelderblom HR, Siegert W & Nitsche A (2000) Rapid quantification of human polyomavirus DNA in undiluted urine from patients after bone marrow transplantation. J Clin Microbiol 38: 3689-3695.
8. Choppa PC, Vojdani A, Tagle C, Andrin R & Magtoto L (1998) Multiplex PCR for the detection of Mycoplasma fermentans, M. hominis and M. penetrans in cell cultures and blood samples of patients with chronic fatigue syndrome. Mol Cell Probes 12: 301-308.
9. Cone RW, Hobson AC & Huang ML (1992) Coamplified positive control detects inhibition of polymerase chain reaction. J Clin Microbiol 30: 3185-3189.
10. Courtney BC, Smith MM & Henchal EA (1999) Development of internal controls for probe-based nucleic acid diagnostic assays. Anal Biochem 270: 249-256.
11. Cubero J, van der Wolf J, Van Beckhoven J & López MM (2002) An internal control for the diagnosis of crown gall by PCR. J Microbiol Methods 51: 387-392.
12. Finney DJ (1971) Probit Analysis, 3rd edn. Cambridge University Press, London.
13. Hartman LJ, Coyne SR & Norwood DA (2005) Development of a novel internal positive control for Taqman based assays. Mol Cell Probes 19: 51-59.
14. Henegariu O, Heerema NA, Dlouhy SR, Vance GH & Vogt PH (1997) Multiplex PCR: critical parameters and step-by-step protocol. Biotechniques 23: 504-511.
15. Hofmann MA (2003) Construction of an infectious chimeric classical swine fever virus containing the 5′UTR of bovine viral diarrhea virus, and its application as a universal internal positive control in real-time RT-PCR. J Virol Methods 114: 77-90.
16. Hoorfar J, Cook N, Malorny B, Wagner M, De Medici D, Abdulmawjood A & Fach P (2003) Making internal amplification control mandatory for diagnostic PCR. J Clin Microbiol 41: 5835.
17. Hoorfar J, Abdulmawjood A, Cook N, Malorny B, Wagner M & Fach P (2004) Practical considerations in design of internal amplification controls for diagnostic PCR assays. J Clin Microbiol 42: 1863-1868.
18. Kimura H, Morita M, Yabuta Y, Kazushima K, Kato K, Kojima S, Matsuyama T & Morishima T (1999) Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. J Clin Microbiol 37: 132-136.
19. King DP, Reid SM, Hutchings GH, Grierson SS, Wilkinson PJ, Dixon LK, Bastos AD & Drew TW (2003) Development of a TaqMan PCR assay with internal amplification control for the detection of African swine fever virus. J Virol Methods 107: 53-61.
20. Kreader CA (1996) Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Appl Environ Microbiol 62: 1102-1106.
21. Leb V, Stöcher M, Valentine-Thon E, Hölzl G, Kessler H, Stekel H & Berg J (2004) Fully automated, internally controlled quantification of hepatitis B virus DNA by real-time PCR by use of the MagNA Pure LC and Light Cycler instruments. J Clin Microbiol 42: 585-590.
22. Lin MH, Chen TC, Kuo TT, Tseng CC & Tseng CP (2000) Real-time PCR for quantitative detection of Toxoplasma gondii. J Clin Microbiol 38: 4121-4125.
23. -ΔΔCt method. Methods 25: 402-408.
24. Maaroufi Y, Ahariz N, Husson M & Crokaert F (2004) Comparison of different methods of isolation of DNA of commonly encountered Candida species and its quantitation by using a real-time PCR-based assay. J Clin Microbiol 42: 3159-3163.
25. Malorny B, Hoorfar J, Bunge C & Helmuth R (2003) Multicenter validation of the analytical accuracy of Salmonella PCR: toward an international standard. Appl Environ Microbiol 69: 290-296.
26. + T lymphocyte response to viral infection during fetal life. J Clin Invest 111: 1747-1755.
27. Niesters HGM (2004) Molecular and diagnostic clinical virology in real time. Clin Microbiol Infect 10: 5-11.
28. Raggam RB, Leitner E, Berg J, Mühlbauer G, Marth E & Kessler HH (2005) Single-run, parallel detection of DNA from three pneumonia-producing bacteria by real-time polymerase chain reaction. J Mol Diagn 7: 133-138.
29. Rosenstraus M, Wang Z, Chang SY, DeBonville D & Spadoro JP (1998) An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance. J Clin Microbiol 36: 191-197.
30. Sachadyn P & Kur J (1998) The construction and use of a PCR internal control. Mol Cell Probes 12: 259-262.
31. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Press, Cold Spring Harbor, NY.
32. Siebert PD & Larrick JW (1993) PCR MIMICS: competitive DNA fragments for use as internal standards in quantitative PCR. Biotechniques 14: 244-249.
33. Stöcher M, Leb V, Hölzl G & Berg J (2002) A simple approach to the generation of heterologous competitive internal controls for real-time PCR assays on the LightCycler. J Clin Virol 25: S47-S53.
34. Stöcher M, Leb V & Berg J (2003) A convenient approach to the generation of multiple internal control DNA for a panel of real-time PCR assays. J Virol Methods 108: 1-8.
35. Stöcher M, Hölzl G, Stekel H & Berg J (2004) Automated detection of five human herpes virus DNAs by a set of LightCycler PCRs complemented with a single multiple internal control. J Clin Virol 29: 171-178.
36. Wang Z, Chang SY, MacMullen G, Huang D, Kwok S & Spadoro J (1994) Use of internal control quantitative standards to quantify target nucleic acid sequences by PCR. 12. Program and Abstracts of the San Diego Conference: the Genetic Revolution. American Society for Clinical Chemistry, Washington DC.