A simple silica-based method for metagenomic DNA extraction from soil and sediments

Molecular Biotechnology

Volumn 40, Issue 1, 2008, Pages 13-17

  Rojas Herrera, R ^a   Narvaez Zapata J ^b   Zamudio Maya, M ^a   Mena Martinez M E ^c  

^a UNIVERSIDAD AUTÓNOMA DE YUCATÁN   (Mexico)

^b INSTITUTO POLITÉCNICO NACIONAL   (Mexico)

^c CENTRO DE INVESTIGACIÓN Y ASISTENCIA EN TECNOLOGÍA Y DISEÑO DEL ESTADO DE JALISCO   (Mexico)

Author keywords

DGGE; Metagenomic DNA; Microbial community; Sediments; Soils

Indexed keywords

ADSORPTION; BACTERIOLOGY; BODY FLUIDS; CESIUM; CESIUM COMPOUNDS; COSMIC RAYS; DNA; ETHANOL; GENES; LACTIC ACID; MAGNESIUM; NUCLEIC ACIDS; PHENOLS; SEDIMENTATION; SEDIMENTOLOGY; SEDIMENTS; SILICA; SOILS;

BACTERIAL DIVERSITIES; BACTERIAL GENOMES; BOVINE SERUM ALBUMINS; CESIUM CHLORIDES; DGGE; ETHANOL PRECIPITATIONS; FIRMICUTES; LACTIC ACID BACTERIA; MAGNESIUM CONCENTRATIONS; METAGENOMIC DNA; METAGENOMIC DNAS; MICROBIAL COMMUNITY; PCR-DGGE; SOIL AND SEDIMENTS; SPECIFIC PRIMERS;

ORGANIC ACIDS;

SILICON DIOXIDE; DNA; PRIMER DNA;

ARTICLE; BACTERIAL GENOME; DNA EXTRACTION; POLYMERASE CHAIN REACTION; SEDIMENT; SOIL; SPECIES DIVERSITY; CHEMISTRY; GENOMICS; ISOLATION AND PURIFICATION; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BACTERIA (MICROORGANISMS); BOVINAE; FIRMICUTES;

BASE SEQUENCE; DNA; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENOMICS; GEOLOGIC SEDIMENTS; POLYMERASE CHAIN REACTION; SILICON DIOXIDE; SOIL;

EID: 55949087075     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-008-9061-8     Document Type: Article

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