A new single-step quantitative pathogen detection system: Template-tagging followed by multiplex asymmetric PCR using common primers and CE-SSCP

Electrophoresis

Volumn 30, Issue 15, 2009, Pages 2728-2736

  Gi, Won Shin ^a   Yang, Sook Cho ^b   Hee, Sung Hwang ^a   Oh, Mi Hwa ^c   Hong, Gil Nam ^a   Jin, Hyun Park ^b   Gyoo, Yeol Jung ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b Food Science Inc ^*  (South Korea)

^c Ewha Womans University   (South Korea)

Author keywords

CE SSCP; Common primer; Diagnosis; Pathogen; Template tagging

Indexed keywords

DIAGNOSIS; DISEASE CONTROL; PATIENT TREATMENT; POLYMERASE CHAIN REACTION;

16S RIBOSOMAL RNA; BACTERIAL INFECTIONS; CE-SSCP; COMMON PRIMER; DETECTION AND QUANTIFICATIONS; DETECTION SYSTEM; MULTIPLEX DETECTIONS; PATHOGEN DETECTION; SINGLE-STEP; TEMPLATE-TAGGING;

PATHOGENS;

COMPLEMENTARY DNA; PRIMER RNA; RIBOSOME RNA; RNA 16S; BACTERIAL RNA; PRIMER DNA;

ARTICLE; BACTERIAL INFECTION; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DNA ISOLATION; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; HUMAN; KLEBSIELLA PNEUMONIAE; MULTIPLEX POLYMERASE CHAIN REACTION; PATHOGENESIS; PROTEUS MIRABILIS; REVERSE TRANSCRIPTION; SEPTICEMIA; SERRATIA MARCESCENS; STENOTROPHOMONAS MALTOPHILIA; STREPTOCOCCUS PYOGENES; BACTERIUM; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SINGLE STRAND CONFORMATION POLYMORPHISM; STATISTICAL MODEL;

BACTERIA; BACTERIAL INFECTIONS; DNA PRIMERS; ELECTROPHORESIS, CAPILLARY; LINEAR MODELS; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; REPRODUCIBILITY OF RESULTS; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY;

EID: 70350786628     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200900074     Document Type: Article

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