Purification of recombinant mandelate racemase: Improved catalytic activity

Protein Expression and Purification

Volumn 69, Issue 1, 2010, Pages 39-46

  Narmandakh, Ariun ^a   Bearne, Stephen L ^a,b  

^a DALHOUSIE UNIVERSITY   (Canada)

^b DALHOUSIE UNIVERSITY   (Canada)

Author keywords

Immobilized metal ion affinity chromatography; Mandelate racemase; Misfolding; Polyhistidine tag; StrepII tag; TIM barrel proteins

Indexed keywords

HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; ISOMERASE; MANDELATE RACEMASE; OLIGOPEPTIDE; RECOMBINANT PROTEIN; STREPTAVIDIN; THROMBIN;

ARTICLE; BIOCATALYSIS; CHEMISTRY; CIRCULAR DICHROISM; DRUG EFFECT; ENZYMOLOGY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN MULTIMERIZATION; PROTEIN PROCESSING; PROTEIN SECONDARY STRUCTURE; PSEUDOMONAS PUTIDA;

BIOCATALYSIS; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HISTIDINE; KINETICS; OLIGOPEPTIDES; PROTEIN MULTIMERIZATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN STRUCTURE, SECONDARY; PSEUDOMONAS PUTIDA; RACEMASES AND EPIMERASES; RECOMBINANT PROTEINS; STREPTAVIDIN; THROMBIN;

PSEUDOMONAS PUTIDA;

EID: 70350568651     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.06.022     Document Type: Article

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