A kinetically trapped intermediate of FK506 binding protein forms in vitro: Chaperone machinery dominates protein folding in vivo

Protein Expression and Purification

Volumn 51, Issue 1, 2007, Pages 80-95

  Wear, Martin A ^a   Patterson, Alan ^a   Walkinshaw, Malcolm D ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

FK506 binding protein; Hexa histidine tags; Peptidyl prolyl isomerase; Rapamycin; Refolding

Indexed keywords

6HL FKBP12 FUSION PROTEIN; 6HL-FKBP12 FUSION PROTEIN; FK 506 BINDING PROTEIN; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; OLIGOPEPTIDE; PEPTIDYLPROLYL ISOMERASE; RAPAMYCIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; HUMAN; KINETICS; METABOLISM; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN RENATURATION;

HISTIDINE; HUMANS; KINETICS; OLIGOPEPTIDES; PEPTIDYLPROLYL ISOMERASE; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN RENATURATION; RECOMBINANT FUSION PROTEINS; SIROLIMUS; TACROLIMUS BINDING PROTEIN 1A; TACROLIMUS BINDING PROTEINS;

EID: 34547601001     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.06.019     Document Type: Article

References (43)

1. Terpe K. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 60 (2003) 523-533
2. Waugh D.S. Making the most of affinity tags. Trends Biotechnol. 23 (2005) 316-320
3. Hunt I. From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein Expr. Purif. 40 (2005) 1-22
4. Doyle S.A. Screening for the expression of soluble recombinant protein in Escherichia coli. Methods Mol. Biol. 310 (2005) 115-121
5. Doyle S.A. High-throughput cloning for proteomics research. Methods Mol. Biol. 310 (2005) 107-113
6. Wu J., and Filutowicz M. Hexahistidine (His6)-tag dependent protein dimerization: a cautionary tale. Acta Biochim. Pol. 46 (1999) 591-599
7. Halliwell C.M., Morgan G., Ou C.P., and Cass A.E. Introduction of a (poly)histidine tag in L-lactate dehydrogenase produces a mixture of active and inactive molecules. Anal Biochem. 295 (2001) 257-261
8. Hartl F.U., and Hayer-Hartl M. Molecular chaperones in the cytosol: from nascent chain to folded protein. Science 295 (2002) 1852-1858
9. Fanghanel J., and Fischer G. Insights into the catalytic mechanism of peptidyl prolyl cis/trans isomerases. Front. Biosci. 9 (2004) 3453-3478
10. Harding M.W., Galat A., Uehling D.E., and Schreiber S.L. A receptor for the immunosuppressant FK506 is a cis-trans peptidyl-prolyl isomerase. Nature 341 (1989) 758-760
11. Galat A. Peptidylprolyl cis/trans isomerases (immunophilins): biological diversity-targets-functions. Curr. Top. Med. Chem. 3 (2003) 1315-1347
12. Schmelzle T., and Hall M.N. TOR a central controller of cell growth. Cell 103 (2000) 253-262
13. Jacinto E., and Hall M.N. Tor signalling in bugs, brain and brawn. Nat. Rev. Mol. Cell Biol. 4 (2003) 117-126
14. Gaburjakova M., Gaburjakova J., Reiken S., Huang F., Marx S.O., Rosemblit N., and Marks A.R. FKBP12 binding modulates ryanodine receptor channel gating. J. Biol. Chem. 276 (2001) 16931-16935
15. Lehnart S.E., Terrenoire C., Reiken S., Wehrens X.H., Song L.S., Tillman E.J., Mancarella S., Cromilas J., Lederer W.J., Kass R.S., and Marks A.R. Stabilization of cardiac ryanodine receptor prevents intracellular calcium leak and arrhythmias. Proc. Natl. Acad. Sci. USA (2006)
16. Huang F., Shan J., Reiken S., Wehrens X.H., and Marks A.R. Analysis of calstabin2 (FKBP12.6)-ryanodine receptor interactions: rescue of heart failure by calstabin2 in mice. Proc. Natl. Acad. Sci. USA 103 (2006) 3456-3461
17. Michnick S.W., Rosen M.K., Wandless T.J., Karplus M., and Schreiber S.L. Solution structure of FKBP, a rotamase enzyme and receptor for FK506 and rapamycin. Science 252 (1991) 836-839
18. VanDuyne G.D., Standaert R.F., Karplus P.A., Schreiber S.L., and Clardy J. Atomic structures of the human immunophilin FKBP-12 complexes with FK506 and rapamycin. J. Mol. Biol. 229 (1993) 105-124
19. Burkhard P., Taylor P., and Walkinshaw M.D. X-ray structures of small ligand-FKBP complexes provide an estimate for hydrophobic interaction energies. J. Mol. Biol. 295 (2000) 953-962
20. Dornan J., Taylor P., and Walkinshaw M.D. Structures of immunophilins and their ligand complexes. Curr. Top Med. Chem. 3 (2003) 1392-1409
21. Egan D.A., Logan T.M., Liang H., Matayoshi E., Fesik S.W., and Holzman T.F. Equilibrium denaturation of recombinant human FK binding protein in urea. Biochemistry 32 (1993) 1920-1927
22. Veeraraghavan S., Holzman T.F., and Nall B.T. Autocatalyzed protein folding. Biochemistry 35 (1996) 10601-10607
23. Main E.R., Fulton K.F., and Jackson S.E. Context-dependent nature of destabilizing mutations on the stability of FKBP12. Biochemistry 37 (1998) 6145-6153
24. Fulton K.F., Main E.R., Daggett V., and Jackson S.E. Mapping the interactions present in the transition state for unfolding/folding of FKBP12. J. Mol. Biol. 291 (1999) 445-461
25. Main E.R., Fulton K.F., and Jackson S.E. Folding pathway of FKBP12 and characterisation of the transition state. J. Mol. Biol. 291 (1999) 429-444
26. Fulton K.F., Jackson S.E., and Buckle A.M. Energetic and structural analysis of the role of tryptophan 59 in FKBP12. Biochemistry 42 (2003) 2364-2372
27. Scholz C., Zarnt T., Kern G., Lang K., Burtscher H., Fischer G., and Schmid F.X. Autocatalytic folding of the folding catalyst FKBP12. J. Biol. Chem. 271 (1996) 12703-12707
28. Fischer G., Bang H., Berger E., and Schellenberger A. Conformational specificity of chymotrypsin toward proline-containing substrates. Biochim. Biophys. Acta 791 (1984) 87-97
29. Fischer G., Wittmann-Liebold B., Lang K., Kiefhaber T., and Schmid F.X. Cyclophilin and peptidyl-prolyl cis-trans isomerase are probably identical proteins. Nature 337 (1989) 476-478
30. Kofron J.L., Kuzmic P., Kishore V., Colon-Bonilla E., and Rich D.H. Determination of kinetic constants for peptidyl-prolyl cis-trans isomerases by an improved spectrophotometric assay. Biochemistry 30 (1991) 6127-6134
31. Dornan J., Page A.P., Taylor P., Wu S., Winter A.D., Husi H., and Walkinshaw M.D. Biochemical and structural characterization of a divergent loop cyclophilin from Caenorhabditis elegans. J. Biol. Chem. 274 (1999) 34877-34883
32. Pace C.N. Determination and analysis of urea and guanidine hydrochloride denaturation curves. Methods Enzymol. 131 (1986) 266-280
33. Schagger H., and vonJagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166 (1987) 368-379
34. Brown R.E., Jarvis K.L., and Hyland K.J. Protein measurement using bicinchoninic acid: elimination of interfering substances. Anal. Biochem. 180 (1989) 136-139
35. Harrison R.K., and Stein R.L. Substrate specificities of the peptidyl prolyl cis-trans isomerase activities of cyclophilin and FK-506 binding protein: evidence for the existence of a family of distinct enzymes. Biochemistry 29 (1990) 3813-3816
36. Harrison R.K., and Stein R.L. Mechanistic studies of peptidyl prolyl cis-trans isomerase: evidence for catalysis by distortion. Biochemistry 29 (1990) 1684-1689
37. Park S.T., Aldape R.A., Futer O., DeCenzo M.T., and Livingston D.J. PPIase catalysis by human FK506-binding protein proceeds through a conformational twist mechanism. J. Biol. Chem. 267 (1992) 3316-3324
38. Kino T., Hatanaka H., Miyata S., Inamura N., Nishiyama M., Yajima T., Goto T., Okuhara M., Kohsaka M., Aoki H., et al. FK-506, a novel immunosuppressant isolated from a Streptomyces. II. Immunosuppressive effect of FK-506 in vitro. J. Antibiot. Tokyo 40 (1987) 1256-1265
39. Graziani F., Aldegheri L., and Terstappen G.C. High throughput scintillation proximity assay for the identification of FKBP-12 ligands. J. Biomol. Screen. 4 (1999) 3-7
40. Sun F., Li P., Ding Y., Wang L., Bartlam M., Shu C., Shen B., Jiang H., Li S., and Rao Z. Design and structure-based study of new potential FKBP12 inhibitors. Biophys. J. 85 (2003) 3194-3201
41. Korepanova A., Douglas C., Leyngold I., and Logan T.M. N-terminal extension changes the folding mechanism of the FK506-binding protein. Protein Sci. 10 (2001) 1905-1910
42. Timerman A.P., Onoue H., Xin H.B., Barg S., Copello J., Wiederrecht G., and Fleischer S. Selective binding of FKBP12.6 by the cardiac ryanodine receptor. J. Biol. Chem. 271 (1996) 20385-20391
43. 2O. Biochemistry 32 (1993) 5583-5590