Introduction of a (poly)histidine tag in L-lactate dehydrogenase produces a mixture of active and inactive molecules

Analytical Biochemistry

Volumn 295, Issue 2, 2001, Pages 257-261

  Halliwell, Catherine M ^a   Morgan, Gareth ^a   Ou, Chung Pei ^a   Cass, Anthony E G ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Affinity chromatography; Bacillus stearothermophilus; Misfolding

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; BACTERIOLOGY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION;

BACILLUS STEAROTHERMOPHILUS; C TERMINUS; HIS-TAG; L-LACTATE; MISFOLDING; UNBOUND FRACTIONS; WILD TYPES;

SIZE EXCLUSION CHROMATOGRAPHY;

ADENOSINE PHOSPHATE; LACTATE DEHYDROGENASE; POLYHISTIDINE; RESIN; SEPHAROSE;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; GEL PERMEATION CHROMATOGRAPHY; GEOBACILLUS STEAROTHERMOPHILUS; MOLECULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING;

GEOBACILLUS STEAROTHERMOPHILUS;

EID: 0035881213     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5182     Document Type: Article

References (20)

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