Expression and purification of active recombinant equine lysozyme in Escherichia coli

Protein Engineering, Design and Selection

Volumn 22, Issue 11, 2009, Pages 649-654

  Časaite, Vida ^a   Bružyte, Simona ^a   Bukauskas, Virginijus ^b   Šetkus, Arunas ^b   Morozova Roche, Ludmilla A ^c   Mekys, Rolandas ^a  

^a VILNIUS UNIVERSITY   (Lithuania)

^b CENTER FOR PHYSICAL SCIENCES AND TECHNOLOGY   (Lithuania)

^c UMEÅ UNIVERSITY   (Sweden)

Author keywords

Amyloid fibrils; Equine lysozyme; Heterologous expression; Inclusion body

Indexed keywords

ACIDIC PH; AMYLOID FIBRIL; AMYLOID FIBRILS; ELEVATED TEMPERATURE; EQUINE LYSOZYME; FUNCTIONAL PROPERTIES; HETEROLOGOUS EXPRESSION; INCLUSION BODIES; INCLUSION BODY; OLIGOMERISATION; PURIFIED PROTEIN; REFOLDING; SYNTHETIC GENES; TEV PROTEASE;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CALCIUM; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; ELECTROLUMINESCENCE; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GLYCOPROTEINS; OLIGOMERIZATION; PROTEIN FOLDING; PURIFICATION;

ENZYMES;

AMYLOID; ARGININE; CALCIUM BINDING PROTEIN; EQUINE LYSOZYME; LYSOZYME; NICKEL; POLYHISTIDINE TAG; PROTEINASE; RECOMBINANT PROTEIN; RECOMBINAT EQUINE LYSOZYME; SEPHAROSE; UNCLASSIFIED DRUG;

ACIDITY; AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CELL INCLUSION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; INCUBATION TIME; NONHUMAN; OLIGOMERIZATION; PH; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; TEMPERATURE;

AMYLOID; ANIMALS; ESCHERICHIA COLI; HORSES; MURAMIDASE; PLASMIDS; RECOMBINANT PROTEINS;

EQUIDAE; ESCHERICHIA COLI; TOBACCO ETCH VIRUS;

EID: 70350464365     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp048     Document Type: Article

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