Recombinant destabilase-lysozyme: Synthesis de novo in E. coli and action mechanism of the enzyme expressed in Spodoptera frugiperda

Biochemistry (Moscow)

Volumn 69, Issue 7, 2004, Pages 776-781

  Zavalova, L L ^a   Baskova, I P ^b   Barsova, E V ^a   Snezhkov, E V ^a   Akopov, S B ^a   Lopatin, S A ^c  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b MOSCOW STATE UNIVERSITY   (Russian Federation)

^c INSTITUTE OF BIOENGINEERING   (Russian Federation)

Author keywords

Destabilase lysozyme; E. Coli expression; Glycosidase activity; Medicinal leech

Indexed keywords

CELL CULTURE; CELLS; DNA; ESCHERICHIA COLI; GENES; GENETIC ENGINEERING; IMMUNOLOGY; MICROORGANISMS; PH EFFECTS; POLYPEPTIDES;

DESTABILASE-ILSOZYME; E.COLI EXPRESSION; GLYCOSIDASE ACTIVITY; MEDICINAL LEECH;

ENZYMES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HIRUDINIDA; HIRUDO MEDICINALIS; INVERTEBRATA; LEPIDOPTERA; SPODOPTERA; SPODOPTERA FRUGIPERDA; STAPHYLOCOCCUS PHAGE 3A; UNIDENTIFIED BACULOVIRUS; VECTORS;

ESCHERICHIA COLI PROTEIN; FIBRIN DESTABILASE; LYSOZYME; N ACETYLGLUCOSAMINE; PROTEINASE; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARMYWORM; ARTICLE; CATALYSIS; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; LEECH; METABOLISM;

ACETYLGLUCOSAMINE; ANIMALS; CATALYSIS; CHROMATOGRAPHY, AFFINITY; ENDOPEPTIDASES; ESCHERICHIA COLI PROTEINS; HIRUDO MEDICINALIS; MURAMIDASE; RECOMBINANT PROTEINS; SPODOPTERA; SUBSTRATE SPECIFICITY;

EID: 4444294168     PISSN: 00062979     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BIRY.0000040203.37624.ef     Document Type: Article

References (27)

1. Zavalova, L. L., Baskova, I. P., Lukyanov, S. A., Sass, A. V., Snezhkov, E. Y., Akopov, S. B., Artamonova, I. I., Archipova, V. S., Nesmeyanov, V. A., Kozlov, D. G., Benevolensky, S. V., Kiseleva, V. I., Poverenny, A. M., and Sverdlov, E. D. (2000) Biochim. Biophys. Acta, 1478, 69-77.
2. Nilsen, I., and Myrnes, B. (2001) Gene, 269, 27-32.
3. Bachali, S., Jager, M., Hassanin, A., Shoentgen, F., Jolles, P., Fiala-Medioni, A., and Deutsch, J. (2002) J. Mol. Evol., 54, 652-664.
4. Nilsen, I., Overbo, K., Sandsdalen, E., Sandaker, E., Sletten, K., and Myrnes, B. (1999) FEES Lett., 464, 153-158.
5. Ito, Y., Yoshikawa, A., Hotani, T., Fukuda, S., Sugimura, K., and Imoto, T. (1999) Eur. J. Biochem., 259, 456-461.
6. Wilson, R., Ainscough, R., Andersen, K., Baynes, C., Berks, M., Bonfield, J., Burton, J., Connell, M., Copsey, T., Cooper, J., et al. (1994) Nature, 368, 32-38.
7. Zavalova, L., Lukyanov, S., Baskova, I., Snezhkov, E., Akopov, S., Berezhnoy, S., Bogdanova, E., Barsova, E., and Sverdlov, E. (1996) Mol. Gen. Genet., 253, 20-25.
8. Fradkov, A., Berezhnoy, S., Barsova, E., Zavalova, L., Lukyanov, S., Baskova, I., and Sverdlov, E. (1996) FEBS Lett., 390, 145-148.
9. Zavalova, L. L., Artamonova, I. I., Berezhnoy, S. N., Tagaev, A. A., Baskova, I. P., Andersen, J., Roepsdorff, P., and Egorov, Ts. A. (2003) Biochem. Biophys. Res. Commun., 306, 318-323.
10. Imoto, T., Johnson, L., North, A., Phillips, D., and Rupley, J. (1972) in The Enzymes (Boyer, P. D., ed.) Academic Press, New York, pp. 665-836.
11. Nurmykhambetova, S., Morozov, I., Chernov, I., and Rubtsov, P. (1996) Mol. Biol. (Moscow), 30, 111-120.
12. Kartasheva, N., Kuchkin, S., and Benevolensky, S. (1996) Yeast, 12, 1297-1300.
13. Laemmli, U. (1970) Nature. 227, 680-685.
14. Coligan, J., Kruisbeek, A., Margulies, D., Shevach, E., and Strober, W. (1998) Current Protocols in Immunology. John Wiley and Sons Inc., New York.
15. Harlow, E., and Lane, D. (1988) Antibodies. A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
16. Turkova, J. (1980) Affinity Chromatography [Russian translation], Mir, Moscow, pp. 188-194.
17. Bradford, M. (1976) Analyt. Biochem., 72, 248-254.
18. Lopatin, S., Ilyin, V., Pustobaev, V., Bezchetnikova, Z., Varlamov, V., and Davankov, V. (1995) Analyt. Biochem., 227, 285-288.
19. Koshiba, T., Hayashi, T., Miwako, I., Kumagai, I., Ikura, T., Kawano, K., Nitta, K., and Kuwajima, K. (1999) Protein Eng., 12, 429-435.
20. Wild, P., Gabrieli, A., Schraner, E., Pellegrini, A., Thomas, U., Frederik, A., Stuart, M., and von Fellenberg, R. (1997) Microscop. Res. Tech., 39, 297-304.
21. Pellegrini, A., Thomas, U., Wild, P., Schraner, E., and von Fellenberg, R. (2000) Microbiol. Res., 155, 69-77.
22. Muraki, M., Harata, K., Hayashi, Y., Machida, M., and Jigami, Y. (1991) Biochim. Biophys. Acta, 1079, 229-237.
23. During, K., Porsh, P., Mahn, A., Bringmann, G., and Giffers, W. (1999) FEBS Lett., 449, 93-100.
24. Hashimoto, Y., Yamada, K., Motoshima, H., Omura, T., Yamada, H., Yasukochi, T., Miki, T., Ueda, T., and Imoto, T. (1996) J. Biochem. (Tokyo), 119, 145-150.
25. Ibrahim, H., Matsuzaki, T., and Aoki, T. (2001) FEES Lett., 506, 27-32.
26. Ibrahim, H., Thomas, U., and Pellegrini, A. (2001) J. Biol. Chem., 276, 43767-43774.
27. Ibrahim, H., Aoki, T., and Pellegrini, A. (2002) Curr. Pharmaceut. Design, 8, 671-693.