Stress responses to heterologous membrane protein expression in Escherichia coli

Biotechnology Letters

Volumn 31, Issue 11, 2009, Pages 1775-1782

  Xu, Lucy Y ^a   Link, A James ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

G protein coupled receptors; Heterologous protein expression; Sigma factor; Stress response

Indexed keywords

CO-EXPRESSION; E. COLI; G PROTEIN-COUPLED RECEPTORS; HEAT-SHOCK; HETEROLOGOUS PROTEIN EXPRESSION; MEMBRANE PROTEINS; PHYSIOLOGICAL MECHANISMS; REAL-TIME DYNAMICS; REPORTER GENE; SIGMA FACTOR; STRESS RESPONSE;

ESCHERICHIA COLI; FLOW CYTOMETRY; GENES; PROTEINS; TRANSCRIPTION;

PHYSIOLOGICAL MODELS;

ADENOSINE TRIPHOSPHATE DEPENDENT PROTEINASE; CANNABINOID 1 RECEPTOR; ESCHERICHIA COLI PROTEIN; FTSH PROTEIN, E COLI; RECOMBINANT PROTEIN; SIGMA FACTOR;

ARTICLE; CYTOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METABOLISM; PHYSIOLOGICAL STRESS; REPORTER GENE;

ATP-DEPENDENT PROTEASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; HUMANS; RECEPTOR, CANNABINOID, CB1; RECOMBINANT PROTEINS; SIGMA FACTOR; STRESS, PHYSIOLOGICAL; TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI;

EID: 70350289424     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0075-5     Document Type: Article

References (27)

1. Bartfai T, Benovic JL, Bockaert J et al (2004) The state of GPCR research in 2004. Nat Rev Drug Discov 3:574-626.
2. Cha HJ, Srivastava R, Vakharia VM, Rao G, Bentley WE (1999) Green fluorescent protein as a noninvasive stress probe in resting Escherichia coli cells. Appl Environ Microbiol 65:409-414.
3. Cherezov V, Rosenbaum DM, Hanson MA et al (2007) High-resolution crystal structure of an engineered human beta(2)-adrenergic G protein-coupled receptor. Science 318:1258-1265.
4. Daley DO, Rapp M, Granseth E et al (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308:1321-1323.
5. Funabashi H, Haruyama T, Mie M et al (2002) Non-destructive monitoring of rpoS promoter activity as stress marker for evaluating cellular physiological status. J Biotechnol 95:85-93.
6. Gruber TM, Gross CA (2003) Multiple sigma subunits and the partitioning of bacterial transcription space. Annu Rev Microbiol 57:441-466.
7. Guzman LM, Belin D, Carson MJ, Beckwith J (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose P-Bad promoter. J Bacteriol 177:4121-4130.
8. Harle C, Kim I, Angerer A, Braun V (1995) Signal transfer through 3 compartments-transcription initiation of the Escherichia coli ferric citrate transport-system from the cell-surface. EMBO J 14:1430-1438.
9. Ito K, Akiyama Y (2005) Cellular functions, mechanism of action, and regulation of FtsH protease. Annu Rev Microbiol 59:211-231.
10. Kostrzynska M, Leung KT, Lee H, Trevors JT (2002) Green fluorescent protein-based biosensor for detecting SOS-inducing activity of genotoxic compounds. J Microbiol Methods 48:43-51.
11. Kundu TK, Kusano S, Ishihama A (1997) Promoter selectivity of Escherichia coli RNA polymerase sigma(F) holoenzyme involved in transcription of flagellar and chemotaxis genes. J Bacteriol 179:4264-4269.
12. Link AJ, Skretas G, Strauch EM, Chari NS, Georgiou G (2008) Efficient expression of membrane-integrated and detergent-soluble G protein-coupled receptors in E. coli. Protein Sci 17:1857-1863.
13. Lu CH, Albano CR, Bentley WE, Rao GV (2005) Quantitative and kinetic study of oxidative stress regulons using green fluorescent protein. Biotechnol Bioeng 89:574-587.
14. Palczewski K, Kumasaka T, Hori T et al (2000) Crystal structure of rhodopsin: a G protein-coupled receptor. Science 289:739-745.
15. Phillips PC, Otto SP, Whitlock MC (2000) Beyond the average: the evolutionary importance of gene interactions and variability of epistatic effects in epistasis and the evolutionary process. Oxford University Press, New York.
16. Rasmussen SGF, Choi HJ, Rosenbaum DM et al (2007) Crystal structure of the human beta(2) adrenergic G-protein-coupled receptor. Nature 450:383-387.
17. Reitzer L, Schneider BL (2001) Metabolic context and possible physiological themes of sigma(54)-dependent genes in Escherichia coli. Microbiol Mol Biol Rev 65:422-444.
18. Ruiz N, Silhavy TJ (2005) Sensing external stress: watchdogs of the Escherichia coli cell envelope. Curr Opin Microbiol 8:122-126.
19. Santini CL, Bernadac A, Zhang M et al (2001) Translocation of jellyfish green fluorescent protein via the Tat system of Escherichia coli and change of its periplasmic localization in response to osmotic up-shock. J Biol Chem 276:8159-8164.
20. Skerra A (1994) Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli. Gene 151:131-135.
21. Tomoyasu T, Gamer J, Bukau B et al (1995) Escherichia coli FtsH is a membrane-bound, ATP-dependent protease which degrades the heat-shock transcription factor sigma(32). EMBO J 14:2551-2560.
22. Wagner S, Bader ML, Drew D, de Gier JW (2006) Rationalizing membrane protein overexpression. Trends Biotechnol 24:364-371.
23. Wagner S, Baars L, Ytterberg AJ et al (2007) Consequences of membrane protein overexpression in Escherichia coli. Mol Cell Proteomics 6:1527-1550.
24. Wang QP, Kaguni JM (1989) A novel sigma-factor is involved in expression of the Rpoh gene of Escherichia coli. J Bacteriol 171:4248-4253.
25. Weber H, Polen T, Heuveling J, Wendisch VF, Hengge R (2005) Genome-wide analysis of the general stress response network in Escherichia coli: sigma(S)-dependent genes, promoters, and sigma factor selectivity. J Bacteriol 187:1591-1603.
26. Zaslaver A, Bren A, Ronen M et al (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3:623-628.
27. Zhao K, Liu MZ, Burgess RR (2005) The global transcriptional response of Escherichia coli to induced sigma(32) protein involves sigma(32) regulon activation followed by inactivation and degradation of sigma(32) in vivo. J Biol Chem 280:17758-17768.