Progenitor cell therapy for heart disease

Experimental Cell Research

Volumn 315, Issue 18, 2009, Pages 3077-3085

  Gonzales, Christine ^a   Pedrazzini, Thierry ^a  

^a University of Lausanne Medical Center   (Switzerland)

Author keywords

Cardiovascular progenitors; Cell therapy; Heart; Stem cells

Indexed keywords

ANGIOGENESIS; BONE MARROW CELL; CARDIAC GRAFT REJECTION; CELL DIFFERENTIATION; CELL REGENERATION; EMBRYONIC STEM CELL; ENDOTHELIUM CELL; EXCITATION CONTRACTION COUPLING; HEART DISEASE; HEART MUSCLE CELL; HEART VENTRICLE REMODELING; HUMAN; MULTIPOTENT STEM CELL; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; REVIEW; STEM CELL MOBILIZATION; STEM CELL TRANSPLANTATION; TERATOMA;

IPS;

EID: 70349786300     PISSN: 00144827     EISSN: 10902422     Source Type: Journal    
DOI: 10.1016/j.yexcr.2009.09.006     Document Type: Review

References (86)

1. Hodges P. Heart failure: epidemiologic update. Crit. Care Nurs. Q. 32 (2009) 24-32
2. Sun Y. Myocardial repair/remodelling following infarction: roles of local factors. Cardiovasc. Res. 81 (2009) 482-490
3. Taylor D.O., Edwards L.B., Aurora P., Christie J.D., Dobbels F., Kirk R., Rahmel A.O., Kucheryavaya A.Y., and Hertz M.I. Registry of the International Society for Heart and Lung Transplantation: twenty-fifth official adult heart transplant report-2008. J. Heart Lung Transplant. 27 (2008) 943-956
4. Augoustides J.G., and Riha H. Recent progress in heart failure treatment and heart transplantation. J. Cardiothorac. Vasc. Anesth. (2009)
5. Beltrami A.P., Urbanek K., Kajstura J., Yan S.M., Finato N., Bussani R., Nadal-Ginard B., Silvestri F., Leri A., Beltrami C.A., and Anversa P. Evidence that human cardiac myocytes divide after myocardial infarction. N. Engl. J. Med. 344 (2001) 1750-1757
6. Anversa P., and Nadal-Ginard B. Myocyte renewal and ventricular remodelling. Nature 415 (2002) 240-243
7. Hsieh P.C., Segers V.F., Davis M.E., MacGillivray C., Gannon J., Molkentin J.D., Robbins J., and Lee R.T. Evidence from a genetic fate-mapping study that stem cells refresh adult mammalian cardiomyocytes after injury. Nat. Med. 13 (2007) 970-974
8. Meilhac S.M., Esner M., Kelly R.G., Nicolas J.F., and Buckingham M.E. The clonal origin of myocardial cells in different regions of the embryonic mouse heart. Dev. Cell 6 (2004) 685-698
9. Kelly R.G., and Buckingham M.E. The anterior heart-forming field: voyage to the arterial pole of the heart. Trends Genet. 18 (2002) 210-216
10. Buckingham M., Meilhac S., and Zaffran S. Building the mammalian heart from two sources of myocardial cells. Nat. Rev., Genet. 6 (2005) 826-835
11. Kelly R.G., Brown N.A., and Buckingham M.E. The arterial pole of the mouse heart forms from Fgf10-expressing cells in pharyngeal mesoderm. Dev. Cell 1 (2001) 435-440
12. Laugwitz K.L., Moretti A., Lam J., Gruber P., Chen Y., Woodard S., Lin L.Z., Cai C.L., Lu M.M., Reth M., Platoshyn O., Yuan J.X., Evans S., and Chien K.R. Postnatal isl1+ cardioblasts enter fully differentiated cardiomyocyte lineages. Nature 433 (2005) 647-653
13. Cai C.L., Liang X., Shi Y., Chu P.H., Pfaff S.L., Chen J., and Evans S. Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart. Dev. Cell 5 (2003) 877-889
14. Snider P., Olaopa M., Firulli A.B., and Conway S.J. Cardiovascular development and the colonizing cardiac neural crest lineage. Sci. World J. 7 (2007) 1090-1113
15. Cai C.L., Martin J.C., Sun Y., Cui L., Wang L., Ouyang K., Yang L., Bu L., Liang X., Zhang X., Stallcup W.B., Denton C.P., McCulloch A., Chen J., and Evans S.M. A myocardial lineage derives from Tbx18 epicardial cells. Nature 454 (2008) 104-108
16. Zhou B., Ma Q., Rajagopal S., Wu S.M., Domian I., Rivera-Feliciano J., Jiang D., von G.A., Ikeda S., Chien K.R., and Pu W.T. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454 (2008) 109-113
17. Srivastava D. Making or breaking the heart: from lineage determination to morphogenesis. Cell 126 (2006) 1037-1048
18. Laugwitz K.L., Moretti A., Caron L., Nakano A., and Chien K.R. Islet1 cardiovascular progenitors: a single source for heart lineages?. Development 135 (2008) 193-205
19. Harvey R.P. NK-2 homeobox genes and heart development. Dev. Biol. 178 (1996) 203-216
20. Charron F., and Nemer M. GATA transcription factors and cardiac development. Semin. Cell Dev. Biol. 10 (1999) 85-91
21. Ema M., Takahashi S., and Rossant J. Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors. Blood 107 (2006) 111-117
22. Moretti A., Caron L., Nakano A., Lam J.T., Bernshausen A., Chen Y., Qyang Y., Bu L., Sasaki M., Martin-Puig S., Sun Y., Evans S.M., Laugwitz K.L., and Chien K.R. Multipotent embryonic isl1+ progenitor cells lead to cardiac, smooth muscle, and endothelial cell diversification. Cell 127 (2006) 1151-1165
23. Sun Y., Liang X., Najafi N., Cass M., Lin L., Cai C.L., Chen J., and Evans S.M. Islet 1 is expressed in distinct cardiovascular lineages, including pacemaker and coronary vascular cells. Dev. Biol. 304 (2007) 286-296
24. Wu S.M., Fujiwara Y., Cibulsky S.M., Clapham D.E., Lien C.L., Schultheiss T.M., and Orkin S.H. Developmental origin of a bipotential myocardial and smooth muscle cell precursor in the mammalian heart. Cell 127 (2006) 1137-1150
25. Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., and Jones J.M. Embryonic stem cell lines derived from human blastocysts. Science 282 (1998) 1145-1147
26. Reubinoff B.E., Pera M.F., Fong C.Y., Trounson A., and Bongso A. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat. Biotechnol. 18 (2000) 399-404
27. Karlsson O., Thor S., Norberg T., Ohlsson H., and Edlund T. Insulin gene enhancer binding protein Isl-1 is a member of a novel class of proteins containing both a homeo- and a Cys-His domain. Nature 344 (1990) 879-882
28. Tsuchida T., Ensini M., Morton S.B., Baldassare M., Edlund T., Jessell T.M., and Pfaff S.L. Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes. Cell 79 (1994) 957-970
29. Thor S., Andersson S.G., Tomlinson A., and Thomas J.B. A LIM-homeodomain combinatorial code for motor-neuron pathway selection. Nature 397 (1999) 76-80
30. Christoforou N., Miller R.A., Hill C.M., Jie C.C., McCallion A.S., and Gearhart J.D. Mouse ES cell-derived cardiac precursor cells are multipotent and facilitate identification of novel cardiac genes. J. Clin. Invest. 118 (2008) 894-903
31. Kattman S.J., Huber T.L., and Keller G.M. Multipotent flk-1+ cardiovascular progenitor cells give rise to the cardiomyocyte, endothelial, and vascular smooth muscle lineages. Dev. Cell 11 (2006) 723-732
32. Yang L., Soonpaa M.H., Adler E.D., Roepke T.K., Kattman S.J., Kennedy M., Henckaerts E., Bonham K., Abbott G.W., Linden R.M., Field L.J., and Keller G.M. Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature 453 (2008) 524-528
33. Grinnemo K.H., Kumagai-Braesch M., Mansson-Broberg A., Skottman H., Hao X., Siddiqui A., Andersson A., Stromberg A.M., Lahesmaa R., Hovatta O., Sylven C., Corbascio M., and Dellgren G. Human embryonic stem cells are immunogenic in allogeneic and xenogeneic settings. Reprod. Biomed. Online 13 (2006) 712-724
34. Grinnemo K.H., Genead R., Kumagai-Braesch M., Andersson A., Danielsson C., Mansson-Broberg A., Dellgren G., Stromberg A.M., Ekberg H., Hovatta O., Sylven C., and Corbascio M. Costimulation blockade induces tolerance to HESC transplanted to the testis and induces regulatory T-cells to HESC transplanted into the heart. Stem Cells 26 (2008) 1850-1857
35. Kolossov E., Bostani T., Roell W., Breitbach M., Pillekamp F., Nygren J.M., Sasse P., Rubenchik O., Fries J.W., Wenzel D., Geisen C., Xia Y., Lu Z., Duan Y., Kettenhofen R., Jovinge S., Bloch W., Bohlen H., Welz A., Hescheler J., Jacobsen S.E., and Fleischmann B.K. Engraftment of engineered ES cell-derived cardiomyocytes but not BM cells restores contractile function to the infarcted myocardium. J. Exp. Med. 203 (2006) 2315-2327
36. Nemir M., Croquelois A., Pedrazzini T., and Radtke F. Induction of cardiogenesis in embryonic stem cells via downregulation of Notch1 signaling. Circ. Res. 98 (2006) 1471-1478
37. Laflamme M.A., Chen K.Y., Naumova A.V., Muskheli V., Fugate J.A., Dupras S.K., Reinecke H., Xu C., Hassanipour M., Police S., O'Sullivan C., Collins L., Chen Y., Minami E., Gill E.A., Ueno S., Yuan C., Gold J., and Murry C.E. Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts. Nat. Biotechnol. 25 (2007) 1015-1024
38. van Laake L.W., Passier R., Monshouwer-Kloots J., Verkleij A.J., Lips D.J., Freund C., den O.K., Ward-van O.D., Korving J., Tertoolen L.G., van Echteld C.J., Doevendans P.A., and Mummery C.L. Human embryonic stem cell-derived cardiomyocytes survive and mature in the mouse heart and transiently improve function after myocardial infarction. Stem Cell Res. 1 (2007) 9-24
39. Leor J., Gerecht S., Cohen S., Miller L., Holbova R., Ziskind A., Shachar M., Feinberg M.S., Guetta E., and Itskovitz-Eldor J. Human embryonic stem cell transplantation to repair the infarcted myocardium. Heart 93 (2007) 1278-1284
40. Tomescot A., Leschik J., Bellamy V., Dubois G., Messas E., Bruneval P., Desnos M., Hagege A.A., Amit M., Itskovitz J., Menasche P., and Puceat M. Differentiation in vivo of cardiac committed human embryonic stem cells in postmyocardial infarcted rats. Stem Cells 25 (2007) 2200-2205
41. Menard C., Hagege A.A., Agbulut O., Barro M., Morichetti M.C., Brasselet C., Bel A., Messas E., Bissery A., Bruneval P., Desnos M., Puceat M., and Menasche P. Transplantation of cardiac-committed mouse embryonic stem cells to infarcted sheep myocardium: a preclinical study. Lancet 366 (2005) 1005-1012
42. Takahashi K., and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126 (2006) 663-676
43. Takahashi K., Tanabe K., Ohnuki M., Narita M., Ichisaka T., Tomoda K., and Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131 (2007) 861-872
44. Yu J., Vodyanik M.A., Smuga-Otto K., Antosiewicz-Bourget J., Frane J.L., Tian S., Nie J., Jonsdottir G.A., Ruotti V., Stewart R., Slukvin I.I., and Thomson J.A. Induced pluripotent stem cell lines derived from human somatic cells. Science 318 (2007) 1917-1920
45. Nakagawa M., Koyanagi M., Tanabe K., Takahashi K., Ichisaka T., Aoi T., Okita K., Mochiduki Y., Takizawa N., and Yamanaka S. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat. Biotechnol. 26 (2008) 101-106
46. Zhang J., Wilson G.F., Soerens A.G., Koonce C.H., Yu J., Palecek S.P., Thomson J.A., and Kamp T.J. Functional cardiomyocytes derived from human induced pluripotent stem cells. Circ. Res. 104 (2009) e30-e41
47. Yu J., Hu K., Smuga-Otto K., Tian S., Stewart R., Slukvin I.I., and Thomson J.A. Human induced pluripotent stem cells free of vector and transgene sequences. Science 324 (2009) 797-801
48. Kaji K., Norrby K., Paca A., Mileikovsky M., Mohseni P., and Woltjen K. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature 458 (2009) 771-775
49. Beltrami A.P., Barlucchi L., Torella D., Baker M., Limana F., Chimenti S., Kasahara H., Rota M., Musso E., Urbanek K., Leri A., Kajstura J., Nadal-Ginard B., and Anversa P. Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell 114 (2003) 763-776
50. Tillmanns J., Rota M., Hosoda T., Misao Y., Esposito G., Gonzalez A., Vitale S., Parolin C., Yasuzawa-Amano S., Muraski J., De A.A., Lecapitaine N., Siggins R.W., Loredo M., Bearzi C., Bolli R., Urbanek K., Leri A., Kajstura J., and Anversa P. Formation of large coronary arteries by cardiac progenitor cells. Proc. Natl. Acad. Sci. U. S. A. 105 (2008) 1668-1673
51. Dawn B., Stein A.B., Urbanek K., Rota M., Whang B., Rastaldo R., Torella D., Tang X.L., Rezazadeh A., Kajstura J., Leri A., Hunt G., Varma J., Prabhu S.D., Anversa P., and Bolli R. Cardiac stem cells delivered intravascularly traverse the vessel barrier, regenerate infarcted myocardium, and improve cardiac function. Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 3766-3771
52. Oh H., Bradfute S.B., Gallardo T.D., Nakamura T., Gaussin V., Mishina Y., Pocius J., Michael L.H., Behringer R.R., Garry D.J., Entman M.L., and Schneider M.D. Cardiac progenitor cells from adult myocardium: homing, differentiation, and fusion after infarction. Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 12313-12318
53. Matsuura K., Nagai T., Nishigaki N., Oyama T., Nishi J., Wada H., Sano M., Toko H., Akazawa H., Sato T., Nakaya H., Kasanuki H., and Komuro I. Adult cardiac Sca-1-positive cells differentiate into beating cardiomyocytes. J. Biol. Chem. 279 (2004) 11384-11391
54. Rosenblatt-Velin N., Lepore M.G., Cartoni C., Beermann F., and Pedrazzini T. FGF-2 controls the differentiation of resident cardiac precursors into functional cardiomyocytes. J. Clin. Invest. 115 (2005) 1724-1733
55. Martin C.M., Meeson A.P., Robertson S.M., Hawke T.J., Richardson J.A., Bates S., Goetsch S.C., Gallardo T.D., and Garry D.J. Persistent expression of the ATP-binding cassette transporter, Abcg2, identifies cardiac SP cells in the developing and adult heart. Dev. Biol. 265 (2004) 262-275
56. Pfister O., Mouquet F., Jain M., Summer R., Helmes M., Fine A., Colucci W.S., and Liao R. CD31 but not CD31 cardiac side population cells exhibit functional cardiomyogenic differentiation. Circ. Res. 97 (2005) 52-61
57. Ott H.C., Matthiesen T.S., Brechtken J., Grindle S., Goh S.K., Nelson W., and Taylor D.A. The adult human heart as a source for stem cells: repair strategies with embryonic-like progenitor cells. Nat. Clin. Pract. Cardiovasc. Med. 4 Suppl. 1 (2007) S27-S39
58. Urbanek K., Quaini F., Tasca G., Torella D., Castaldo C., Nadal-Ginard B., Leri A., Kajstura J., Quaini E., and Anversa P. Intense myocyte formation from cardiac stem cells in human cardiac hypertrophy. Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 10440-10445
59. Quaini F., Urbanek K., Beltrami A.P., Finato N., Beltrami C.A., Nadal-Ginard B., Kajstura J., Leri A., and Anversa P. Chimerism of the transplanted heart. N. Engl. J. Med. 346 (2002) 5-15
60. Bearzi C., Rota M., Hosoda T., Tillmanns J., Nascimbene A., De A.A., Yasuzawa-Amano S., Trofimova I., Siggins R.W., Lecapitaine N., Cascapera S., Beltrami A.P., D'Alessandro D.A., Zias E., Quaini F., Urbanek K., Michler R.E., Bolli R., Kajstura J., Leri A., and Anversa P. Human cardiac stem cells. Proc. Natl. Acad. Sci. U. S. A. 104 (2007) 14068-14073
61. Pouly J., Bruneval P., Mandet C., Proksch S., Peyrard S., Amrein C., Bousseaux V., Guillemain R., Deloche A., Fabiani J.N., and Menasche P. Cardiac stem cells in the real world. J. Thorac. Cardiovasc. Surg. 135 (2008) 673-678
62. Messina E., De A.L., Frati G., Morrone S., Chimenti S., Fiordaliso F., Salio M., Battaglia M., Latronico M.V., Coletta M., Vivarelli E., Frati L., Cossu G., and Giacomello A. Isolation and expansion of adult cardiac stem cells from human and murine heart. Circ. Res. 95 (2004) 911-921
63. Smith R.R., Barile L., Cho H.C., Leppo M.K., Hare J.M., Messina E., Giacomello A., Abraham M.R., and Marban E. Regenerative potential of cardiosphere-derived cells expanded from percutaneous endomyocardial biopsy specimens. Circulation 115 (2007) 896-908
64. Andersen D.C., Andersen P., Schneider M., Jensen H.B., and Sheikh S.P. Murine "Cardiospheres" are not a source of stem cells with cardiomyogenic potential. Stem Cells 27 (2009) 1571-1581
65. Goumans M.J., de Boer T.P., Smits A.M., van Laake L.W., van V.P., Metz C.H., Korfage T.H., Kats K.P., Hochstenbach R., Pasterkamp G., Verhaar M.C., van der Heyden M.A., de K.D., Mummery C.L., van Veen T.A., Sluijter J.P., and Doevendans P.A. TGF-beta1 induces efficient differentiation of human cardiomyocyte progenitor cells into functional cardiomyocytes in vitro. Stem Cell Res. 1 (2007) 138-149
66. Orlic D., Kajstura J., Chimenti S., Jakoniuk I., Anderson S.M., Li B., Pickel J., McKay R., Nadal-Ginard B., Bodine D.M., Leri A., and Anversa P. Bone marrow cells regenerate infarcted myocardium. Nature 410 (2001) 701-705
67. Murry C.E., Soonpaa M.H., Reinecke H., Nakajima H., Nakajima H.O., Rubart M., Pasumarthi K.B., Virag J.I., Bartelmez S.H., Poppa V., Bradford G., Dowell J.D., Williams D.A., and Field L.J. Haematopoietic stem cells do not transdifferentiate into cardiac myocytes in myocardial infarcts. Nature 428 (2004) 664-668
68. Balsam L.B., Wagers A.J., Christensen J.L., Kofidis T., Weissman I.L., and Robbins R.C. Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium. Nature 428 (2004) 668-673
69. Nygren J.M., Jovinge S., Breitbach M., Sawen P., Roll W., Hescheler J., Taneera J., Fleischmann B.K., and Jacobsen S.E. Bone marrow-derived hematopoietic cells generate cardiomyocytes at a low frequency through cell fusion, but not transdifferentiation. Nat. Med. 10 (2004) 494-501
70. Badorff C., Brandes R.P., Popp R., Rupp S., Urbich C., Aicher A., Fleming I., Busse R., Zeiher A.M., and Dimmeler S. Transdifferentiation of blood-derived human adult endothelial progenitor cells into functionally active cardiomyocytes. Circulation 107 (2003) 1024-1032
71. Young P.P., Vaughan D.E., and Hatzopoulos A.K. Biologic properties of endothelial progenitor cells and their potential for cell therapy. Prog. Cardiovasc. Dis. 49 (2007) 421-429
72. Narmoneva D.A., Vukmirovic R., Davis M.E., Kamm R.D., and Lee R.T. Endothelial cells promote cardiac myocyte survival and spatial reorganization: implications for cardiac regeneration. Circulation 110 (2004) 962-968
73. Psaltis P.J., Zannettino A.C., Worthley S.G., and Gronthos S. Concise review: mesenchymal stromal cells: potential for cardiovascular repair. Stem Cells 26 (2008) 2201-2210
74. van der Bogt K.E., Schrepfer S., Yu J., Sheikh A.Y., Hoyt G., Govaert J.A., Velotta J.B., Contag C.H., Robbins R.C., and Wu J.C. Comparison of transplantation of adipose tissue- and bone marrow-derived mesenchymal stem cells in the infarcted heart. Transplantation 87 (2009) 642-652
75. Lipinski M.J., Biondi-Zoccai G.G., Abbate A., Khianey R., Sheiban I., Bartunek J., Vanderheyden M., Kim H.S., Kang H.J., Strauer B.E., and Vetrovec G.W. Impact of intracoronary cell therapy on left ventricular function in the setting of acute myocardial infarction: a collaborative systematic review and meta-analysis of controlled clinical trials. J. Am. Coll. Cardiol. 50 (2007) 1761-1767
76. Rubart M., and Field L.J. Stem cell differentiation: cardiac repair. Cells Tissues Organs 188 (2008) 202-211
77. Murry C.E., Field L.J., and Menasche P. Cell-based cardiac repair: reflections at the 10-year point. Circulation 112 (2005) 3174-3183
78. Ceradini D.J., and Gurtner G.C. Homing to hypoxia: HIF-1 as a mediator of progenitor cell recruitment to injured tissue. Trends Cardiovasc. Med. 15 (2005) 57-63
79. Zaruba M.M., Theiss H.D., Vallaster M., Mehl U., Brunner S., David R., Fischer R., Krieg L., Hirsch E., Huber B., Nathan P., Israel L., Imhof A., Herbach N., Assmann G., Wanke R., Mueller-Hoecker J., Steinbeck G., and Franz W.M. Synergy between CD26/DPP-IV inhibition and G-CSF improves cardiac function after acute myocardial infarction. Cell Stem Cell 4 (2009) 313-323
80. Segers V.F., Tokunou T., Higgins L.J., MacGillivray C., Gannon J., and Lee R.T. Local delivery of protease-resistant stromal cell derived factor-1 for stem cell recruitment after myocardial infarction. Circulation 116 (2007) 1683-1692
81. Krause K., Jaquet K., Schneider C., Haupt S., Lioznov M.V., Otte K.M., and Kuck K.H. Percutaneous intramyocardial stem cell injection in patients with acute myocardial infarction: first-in-man study. Heart 95 (2009) 1145-1152
82. Frantz S., Bauersachs J., and Ertl G. Post-infarct remodelling: contribution of wound healing and inflammation. Cardiovasc. Res. 81 (2009) 474-481
83. Martens T.P., Godier A.F., Parks J.J., Wan L.Q., Koeckert M.S., Eng G.M., Hudson B.I., Sherman W., and Vunjak-Novakovic G. Percutaneous cell delivery into the heart using hydrogels polymerizing in situ. Cell Transplant 18 (2009) 297-304
84. Menasche P. Skeletal myoblasts as a therapeutic agent. Prog. Cardiovasc. Dis. 50 (2007) 7-17
85. Noiseux N., Gnecchi M., Lopez-Ilasaca M., Zhang L., Solomon S.D., Deb A., Dzau V.J., and Pratt R.E. Mesenchymal stem cells overexpressing Akt dramatically repair infarcted myocardium and improve cardiac function despite infrequent cellular fusion or differentiation. Mol. Ther. 14 (2006) 840-850
86. Lu G., Haider H.K., Jiang S., and Ashraf M. Sca-1+ stem cell survival and engraftment in the infarcted heart: dual role for preconditioning-induced connexin-43. Circulation 119 (2009) 2587-2596