The effect of cell concentration on alpha 2,3-sialyltransferase activity in attachment culture of a human erythropoietin-producing Chinese hamster ovary cell line

Biotechnology and Bioprocess Engineering

Volumn 14, Issue 4, 2009, Pages 406-413

  Kim, Hyoung Jin ^a   Kim, Hong Jin ^a  

^a Chung Ang University   (South Korea)

Author keywords

2,3 sialyltransferase; Ammonium; Chinese hamster ovary; Erythropoietin; Glycoprotein; Terminal sialylation

Indexed keywords

AMMONIUM; AMMONIUM CONCENTRATIONS; AREA-BASED; BIOLOGICAL ACTIVITIES; CELL ATTACHMENTS; CELL CONCENTRATIONS; CHINESE HAMSTER OVARY; CHINESE HAMSTER OVARY CELLS; CHO CELL; CULTURE MEDIUM; ERYTHROPOIETIN; GLYCANS; HUMAN ERYTHROPOIETIN; IN-VIVO; KEY ENZYMES; RECOMBINANT HUMAN ERYTHROPOIETIN (RHEPO); SIALIC ACIDS; TERMINAL SIALYLATION;

AMMONIUM COMPOUNDS; CARBOXYLIC ACIDS; CELL MEMBRANES; CONCENTRATION (PROCESS); ENZYMES; GLYCOPROTEINS;

CELL CULTURE;

ALPHA 2,3 SIALYLTRANSFERASE; AMMONIA; AMMONIUM CHLORIDE; RECOMBINANT ERYTHROPOIETIN; SIALYLTRANSFERASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CELL ADHESION; CELL CONTACT; CELL COUNT; CELL DENSITY; CELL PROLIFERATION; CHO CELL; CONCENTRATION (PARAMETERS); CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; FEMALE; GROWTH RATE; NONHUMAN; PROTEIN SYNTHESIS; SIALYLATION;

CRICETULUS GRISEUS;

EID: 70349648072     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-009-0050-y     Document Type: Article

References (31)

1. Grabenhorst, E., P. Schlenke, S. Pohl, M. Nimtz, and H. S. Conradt (1999) Genetic engineering of recombinant glycoproteins and the glycosylation pathway in mammalian host cells. Glycoconj. J. 16: 81-97.
2. Kim, I. K., D. J. Lim, and H. W. Park (2008) Effects of synthetic lysophosphatidylcholines on suspension cultures of the Chinese hamster ovary DG44 cells in protein-free media. Biotechnol. Bioprocess Eng. 13: 396-400.
3. Lee, J. C., D. Y. Kim, D. J. Oh, and H. N. Chang (2008) Long-term operation of depth filter perfusion systems (DFPS) for monoclonal antibody production using recombinant CHO cells: Effect of temperature, pH, and dissolved oxygen. Biotechnol. Bioprocess Eng. 13: 401-409.
4. Varki, A. (1993) Biological roles of oligosaccharides: All of the theories are correct. Glycobiology 3: 97-130.
5. Kim, H. J. and H. J. Kim (2006) Glycosylation variant analysis of recombinant human tissue plasminogen activator produced in urea-cycle-enzyme-expressing Chinese hamster ovary (CHO) cell line. J. Biosci. Bioeng. 102: 447-451.
6. Paccalet, T., M. Bardor, C. Rihouey, F. Delmas, C. Chevalier, M. A. D'Aoust, L. Faye, L. Vezina, V. Gomord, and P. Lerouge (2007) Engineering of a sialic acid synthesis pathway in transgenic plants by expression of bacterial Neu5Ac-synthesizing enzymes. Plant Biotechnol. J. 5: 16-25.
7. Shah, M. M., K. Fujiyama, C. R. Flynn, and L. Joshi (2003) Sialylated endogenous glycoconjugates in plant cells. Nat. Biotechnol. 21: 1470-1471.
8. Chen, M., X. Liu, Z. Wang, J. Song, Q. Qi, and P. G. Wang (2005) Modification of plant N-glycans processing: The future of producing therapeutic protein by transgenic plants. Med. Res. Rev. 25: 343-360.
9. Restelli, V., M. D. Wang, N. Huzel, M. Ethier, H. Perreault, and M. Butler (2006) The effect of dissolved oxygen on the production and the glycosylation profile of recombinant human erythropoietin produced from CHO cells. Biotechnol. Bioeng. 94: 481-494.
10. Cointe, D., R. Beliard, S. Jorieux, Y. Leroy, A. Glacet, A. Verbert, D. Bourel, and F. Chirat (2000) Unusual N-glycosylation of a recombinant human erythropoietin expressed in a human lymphoblastoid cell line does not alter its biological properties. Glycobiology 10: 511-519.
11. Davis, J. M., T. Arakawa, T. W. Strickland, and D. A. Yphantis (1987) Characterization of recombinant human erythropoietin produced in Chinese hamster ovary cells. Biochemistry 26: 2633-2638.
12. Yamaguchi, K., K. Akai, G. Kawanishi, M. Ueda, S. Masuda, and R. Sasaki (1991) Effects of site-directed removal of N-glycosylation sites in human erythropoietin on its production and biological properties. J. Biol. Chem. 266: 20434-20439.
13. Fukuda, M. N., H. Sasaki, L. Lopez, and M. Fukuda (1989) Survival of recombinant erythropoietin in the circulation: The role of carbohydrates. Blood 73: 84-89.
14. Gawlitzek, M., T. Ryll, J. Lofgren, and M. B. Sliwkowski (2000) Ammonium alters N-glycan structures of recombinant TNFR-IgG: Degradative versus biosynthetic mechanisms. Biotechnol. Bioeng. 68: 637-646.
15. Goochee, C. F., M. J. Gramer, D. C. Andersen, J. B. Bahr, and J. R. Rasmussen (1991) The oligosaccharides of glycoproteins: Bioprocess factors affecting oligosaccharide structure and their effect on glycoprotein properties. Biotechnology 9: 1347-1355.
16. Schneider, M., I. W. Marison, and U. von Stockar (1996) The importance of ammonia in mammalian cell culture. J. Biotechnol. 46: 161-185.
17. Kim, N., Y. Lee, H. Kim, J. Choi, J. Kim, K. H. Chang, J. H. Kim, and H. J. Kim (2004) Enhancement of erythropoietin production from Chinese hamster ovary (CHO) cells by introduction of the urea cycle enzymes, carbamoyl phosphate synthetase I, and ornithine transcarbamylase. J. Microbiol. Biotechnol. 14: 844-851.
18. Baker, K. N., M. H. Rendall, A. E. Hills, M. Hoare, R. B. Freedman, and D. C. James (2001) Metabolic control of recombinant protein N-glycan processing in NS0 and CHO cells. Biotechnol. Bioeng. 73: 188-202.
19. Hills, A. E., A. Patel, P. Boyd, and D. C. James (2001) Metabolic control of recombinant monoclonal antibody N-glycosylation in GS-NS0 cells. Biotechnol. Bioeng. 75: 239-251.
20. Park, H., I. H. Kim, I. Y. Kim, K. H. Kim, and H. J. Kim (2000) Expression of carbamoyl phosphate synthetase I and ornithine transcarbamoylase genes in Chinese hamster ovary dhfr-cells decreases accumulation of ammonium ion in culture media. J. Biotechnol. 81: 129-140.
21. Wakisaka, S., T. Tachiki, H. C. Sung, H. Kumagai, T. Tochikura, and S. Matsui (1987) A rapid assay method for ammonia using glutamine synthetase from glutamate-producing bacteria. Anal. Biochem. 163: 117-122.
22. Kim, H. J. and H. J. Kim (2007) The glycosylation and pharmacokinetics of CTLA4Ig produced in rice cells. Biol. Pharm. Bull. 30: 1913-1917.
23. Ohta, Y., Y. Tsukada, and T. Sugimori (1989) Purification and properties of neuraminidase isozymes in Arthrobacter ureafaciens mutant. J. Biochem. 106: 1086-1089.
24. Basset, C., V. Durand, N. Mimassi, Y. L. Pennec, P. Youinou, and M. Dueymes (2000) Enhanced sialyltransferase activity in B lymphocytes from patients with primary Sjogren's syndrome. Scand. J. Immunol. 51: 307-311.
25. Kim, N. Y., J. H. Kim, and H. J. Kim (2005) Effect of low adapted temperature and medium composition on growth and erythropoietin (EPO) production by Chinese hamster ovary cells. Arch. Pharm. Res. 28: 220-226.
26. Kim, H. J., S. J. Lee, and H. J. Kim (2008) Antibody-based enzyme-linked lectin assay (ABELLA) for the sialylated recombinant human erythropoietin present in culture supernatant. J. Pharm. Biomed. Anal. 48: 716-721.
27. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
28. Yang, M. and M. Butler (2000) Effects of ammonia on CHO cell growth, erythropoietin production, and glycosylation. Biotechnol. Bioeng. 68: 370-380.
29. Hwang, S. O. and G. M. Lee (2008) Nutrient deprivation induces autophagy as well as apoptosis in Chinese hamster ovary cell culture. Biotechnol. Bioeng. 99: 678-685.
30. Andersen, D. C. and C. F. Goochee (1995) The effect of ammonia on the O-linked glycosylation of granulocytecolony-stimulating factor produced by chinese-hamster ovary cells. Biotechnol. Bioeng. 47: 96-105.
31. Borys, M. C., D. I. Linzer, and E. T. Papoutsakis (1994) Ammonia affects the glycosylation patterns of recombinant mouse placental lactogen-I by chinese hamster ovary cells in a pH-dependent manner. Biotechnol. Bioeng. 43: 505-514.