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Volumn 10, Issue 5, 2000, Pages 511-519

Unusual N-glycosylation of a recombinant human erythropoietin expressed in a human lymphoblastoid cell line does not alter its biological properties

Author keywords

Biological activity; Electrospray ionization mass spectrometry; Glycosylation; Recombinant glycoprotein; Sialylation

Indexed keywords

AMINO ACID; GLYCAN; GLYCOSYLATED PROTEIN; RECOMBINANT ERYTHROPOIETIN;

EID: 0343134556     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/10.5.511     Document Type: Article
Times cited : (31)

References (45)
  • 2
    • 0021749885 scopus 로고
    • A dominant mutation to ricin resistance in Chinese hamster ovary cells induces UDP-GlcNAc:Glycopeptide β-4-N-acetylglucosaminyltransferase III activity
    • Campbell, C. and Stanley, P. (1984) A dominant mutation to ricin resistance in Chinese hamster ovary cells induces UDP-GlcNAc:glycopeptide β-4-N-acetylglucosaminyltransferase III activity. J. Biol. Chem., 259, 13370-13378.
    • (1984) J. Biol. Chem. , vol.259 , pp. 13370-13378
    • Campbell, C.1    Stanley, P.2
  • 3
    • 0031691319 scopus 로고    scopus 로고
    • Determination of the sialylation level and of the ratio α-(2→3)/α-(2→6) sialyl linkages of N-glycans by methylation and GC/MS analysis
    • Cointe, D., Leroy, Y. and Chirat, F. (1998) Determination of the sialylation level and of the ratio α-(2→3)/α-(2→6) sialyl linkages of N-glycans by methylation and GC/MS analysis. Carbohydr. Res., 311, 51-59.
    • (1998) Carbohydr. Res. , vol.311 , pp. 51-59
    • Cointe, D.1    Leroy, Y.2    Chirat, F.3
  • 4
    • 0020479688 scopus 로고
    • Characterization of the structural determinants required for the high affinity interaction of asparagine-linked oligosaccharides with immobilized Phaseolus vulgaris leukoagglutinating and erythroagglutinating lectins
    • Cummings, R.D. and Kornfeld, S. (1982) Characterization of the structural determinants required for the high affinity interaction of asparagine-linked oligosaccharides with immobilized Phaseolus vulgaris leukoagglutinating and erythroagglutinating lectins. J. Biol. Chem., 257, 11230-11234.
    • (1982) J. Biol. Chem. , vol.257 , pp. 11230-11234
    • Cummings, R.D.1    Kornfeld, S.2
  • 6
    • 0021908684 scopus 로고
    • The role of carbohydrate in erythropoietin action
    • Dordal, M.S., Wang, F.F. and Goldwasser, E. (1985) The role of carbohydrate in erythropoietin action. Endocrinology, 116, 2293-2299.
    • (1985) Endocrinology , vol.116 , pp. 2293-2299
    • Dordal, M.S.1    Wang, F.F.2    Goldwasser, E.3
  • 7
    • 0024270999 scopus 로고
    • Glycosylation at specific sites of erythropoietin is essential for biosynthesis, secretion and biological function
    • Dubè, S., Fisher, J.W. and Powell, J.S. (1988) Glycosylation at specific sites of erythropoietin is essential for biosynthesis, secretion and biological function. J. Biol. Chem., 263, 17516-17521.
    • (1988) J. Biol. Chem. , vol.263 , pp. 17516-17521
    • Dubè, S.1    Fisher, J.W.2    Powell, J.S.3
  • 8
    • 0024497455 scopus 로고
    • Survival of recombinant erythropoietin in the circulation: The role of carbohydrates
    • Fukuda, M.N., Sasaki, H., Lopez, L. and Fukuda, M. (1989) Survival of recombinant erythropoietin in the circulation: the role of carbohydrates. Blood, 73, 84-89.
    • (1989) Blood , vol.73 , pp. 84-89
    • Fukuda, M.N.1    Sasaki, H.2    Lopez, L.3    Fukuda, M.4
  • 9
    • 0023175723 scopus 로고
    • Fluorometric high-performance liquid chromatography of N-acetyl-and N-glycolylneuraminic acids and its application to their microdetermination in human and animal sera, glycoproteins and glycolipids
    • Hara, S., Takemori, Y., Yamaguchi, M., Nakamura, M. and Ohkura, Y. (1987) Fluorometric High-Performance Liquid Chromatography of N-acetyl-and N-glycolylneuraminic acids and its application to their microdetermination in human and animal sera, glycoproteins and glycolipids. Anal. Biochem., 164, 138-145.
    • (1987) Anal. Biochem. , vol.164 , pp. 138-145
    • Hara, S.1    Takemori, Y.2    Yamaguchi, M.3    Nakamura, M.4    Ohkura, Y.5
  • 10
    • 0026981076 scopus 로고
    • Simple in vivo bioassay without radioisotopes for recombinant human erythropoietins
    • Hayakawa, T., Wada, M., Mizuno, K., Abe, S., Miyashita, M. and Ueda, M. (1992) Simple in vivo bioassay without radioisotopes for recombinant human erythropoietins. Biologicals, 20, 243-251.
    • (1992) Biologicals , vol.20 , pp. 243-251
    • Hayakawa, T.1    Wada, M.2    Mizuno, K.3    Abe, S.4    Miyashita, M.5    Ueda, M.6
  • 11
    • 0026702568 scopus 로고
    • Role of sugar chains in the expression of the biological activity of human erythropoeitin
    • Higuchi, M., Oh-eda, M., Kuboniwa, H., Tomonoh, K., Shimonaka, Y. and Ochi, N. (1992) Role of sugar chains in the expression of the biological activity of human erythropoeitin. J. Biol. Chem., 267, 7703-7709.
    • (1992) J. Biol. Chem. , vol.267 , pp. 7703-7709
    • Higuchi, M.1    Oh-Eda, M.2    Kuboniwa, H.3    Tomonoh, K.4    Shimonaka, Y.5    Ochi, N.6
  • 12
    • 0025184040 scopus 로고
    • Sialylated carbohydrate chains of recombinant human glycoproteins expressed in Chinese hamster ovary cells contain traces of N-glycolylneuraminic acid
    • Hokke, C.H., Bergwerff, A.A., van Dedem, G.W.K., van Oostrum, J., Kamerling, J.P. and Vliegenthart, J.F.G. (1990) Sialylated carbohydrate chains of recombinant human glycoproteins expressed in Chinese hamster ovary cells contain traces of N-glycolylneuraminic acid. FEBS Lett., 275, 9-14.
    • (1990) FEBS Lett. , vol.275 , pp. 9-14
    • Hokke, C.H.1    Bergwerff, A.A.2    Van Dedem, G.W.K.3    Van Oostrum, J.4    Kamerling, J.P.5    Vliegenthart, J.F.G.6
  • 13
    • 0028957067 scopus 로고
    • Structural analysis of the sialylated N-and O-linked carbohydrate chains of recombinant human erythropoietin expressed in Chinese Hamster Ovary cells. Sialylation patterns and branch of N-acetyl-lactosamine units
    • Hokke, C.H., Bergwerff, A.A., van Dedem, G.W.K., Kamerling, J.P. and Vliegenthart, J.F.G. (1995) Structural analysis of the sialylated N-and O-linked carbohydrate chains of recombinant human erythropoietin expressed in Chinese Hamster Ovary cells. Sialylation patterns and branch of N-acetyl-lactosamine units. Eur. J. Biochem., 228, 981-1008.
    • (1995) Eur. J. Biochem. , vol.228 , pp. 981-1008
    • Hokke, C.H.1    Bergwerff, A.A.2    Van Dedem, G.W.K.3    Kamerling, J.P.4    Vliegenthart, J.F.G.5
  • 14
    • 0025646750 scopus 로고
    • Physicochemical and biological characterization of asialoerythropoietin. Suppressive effects of sialic acid in the expression of biological activity of human erythropoietin in vitro
    • Imai, N., Higuchi, M., Kawamura, A., Tomonoh, K., Oh-eda, M., Fujiwara, M., Shimonaka, Y. and Ochi, N. (1990) Physicochemical and biological characterization of asialoerythropoietin. Suppressive effects of sialic acid in the expression of biological activity of human erythropoietin in vitro. Eur. J. Biochem., 194, 457-462.
    • (1990) Eur. J. Biochem. , vol.194 , pp. 457-462
    • Imai, N.1    Higuchi, M.2    Kawamura, A.3    Tomonoh, K.4    Oh-Eda, M.5    Fujiwara, M.6    Shimonaka, Y.7    Ochi, N.8
  • 16
    • 0026526168 scopus 로고
    • Erythropoietin: Structure, control of production and function
    • Jelkmann, W. (1992) Erythropoietin: structure, control of production and function Physiol. Rev., 72, 449-489.
    • (1992) Physiol. Rev. , vol.72 , pp. 449-489
    • Jelkmann, W.1
  • 17
    • 0030902350 scopus 로고    scopus 로고
    • New sialic acids from biological sources identified by a comprehensive and sensitive approach: Liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) of SIA quinoxalinones
    • Klein, A., Diaz, S., Ferreira, I., Lamblin, G., Raussel, P. and Manzi, A.E. (1997) New sialic acids from biological sources identified by a comprehensive and sensitive approach: liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) of SIA quinoxalinones. Glycobiology, 7, 421-432.
    • (1997) Glycobiology , vol.7 , pp. 421-432
    • Klein, A.1    Diaz, S.2    Ferreira, I.3    Lamblin, G.4    Raussel, P.5    Manzi, A.E.6
  • 19
    • 0028284572 scopus 로고
    • Applications of electrospray mass spectrometry to erythropoietin N-and O-linked glycans
    • Linsley, K.B., Chan, S.-Y., Chan, S., Reinhold, B.B., Lisi, P.J. and Reinhold, V.N. (1994) Applications of electrospray mass spectrometry to erythropoietin N-and O-linked glycans. Anal. Biochem., 219, 207-217.
    • (1994) Anal. Biochem. , vol.219 , pp. 207-217
    • Linsley, K.B.1    Chan, S.-Y.2    Chan, S.3    Reinhold, B.B.4    Lisi, P.J.5    Reinhold, V.N.6
  • 20
    • 0025018353 scopus 로고
    • ELAM-1-dependent cell adhesion to vascularendothelium determined by a transfected human fucosyltransferase cDNA
    • Lowe, J.B., Stoolman, L.M., Nair, R.P., Larsen, R.D., Berhend, T.L. and Marks, R.M. (1990) ELAM-1-dependent cell adhesion to vascularendothelium determined by a transfected human fucosyltransferase cDNA. Cell, 63, 475-484.
    • (1990) Cell , vol.63 , pp. 475-484
    • Lowe, J.B.1    Stoolman, L.M.2    Nair, R.P.3    Larsen, R.D.4    Berhend, T.L.5    Marks, R.M.6
  • 21
    • 0029751344 scopus 로고    scopus 로고
    • Biological and physicochemical characterization of recombinant human erythropoietins fractionated by Mono Q column chromatography and their modification with sialyltransferase
    • Morimoto, K., Tsuda, E., Said Abdu, A., Uchida, E., Hatakeyama, S., Ueda, M. and Hayakawa, T. (1996) Biological and physicochemical characterization of recombinant human erythropoietins fractionated by Mono Q column chromatography and their modification with sialyltransferase. Glycogonjugate J., 13, 1013-1020.
    • (1996) Glycogonjugate J. , vol.13 , pp. 1013-1020
    • Morimoto, K.1    Tsuda, E.2    Said Abdu, A.3    Uchida, E.4    Hatakeyama, S.5    Ueda, M.6    Hayakawa, T.7
  • 22
    • 0027171780 scopus 로고
    • Protective effects of oligosaccharides in P-selectin-dependent lung injury
    • Mulligan, M.S., Paulson, J.C., De Frees, S., Zheng, Z.L., Lowe, J.B. and Ward, P.A. (1993) Protective effects of oligosaccharides in P-selectin-dependent lung injury. Nature, 364, 149-151.
    • (1993) Nature , vol.364 , pp. 149-151
    • Mulligan, M.S.1    Paulson, J.C.2    De Frees, S.3    Zheng, Z.L.4    Lowe, J.B.5    Ward, P.A.6
  • 23
    • 0023685081 scopus 로고
    • β-1,4-Mannosyl-glycoprotein β-1,4-N-acetylglucosaminyltransferase III activity in human B and T lymphocyte lines and in tonsillar B and T lymphocytes
    • Narasimhan, S., Lee, J.W., Cheung, R.K., Gelfand, E.W. and Schachter, H. (1988) β-1,4-Mannosyl-glycoprotein β-1,4-N-acetylglucosaminyltransferase III activity in human B and T lymphocyte lines and in tonsillar B and T lymphocytes. Biochem. Cell Biol., 66, 889-900.
    • (1988) Biochem. Cell Biol. , vol.66 , pp. 889-900
    • Narasimhan, S.1    Lee, J.W.2    Cheung, R.K.3    Gelfand, E.W.4    Schachter, H.5
  • 24
    • 0027849717 scopus 로고
    • Dependance for the proliferative response to erythropoietin on an established erythroid differentiation program in a human hematopoietic cell line. UT-7
    • Nicolis, S., Ottolenghi, S., Papayannopoulos, T., Baiocchi, M., Migliaccio, G., Adamson, J. and Migliaccio, A.R. (1993) Dependance for the proliferative response to erythropoietin on an established erythroid differentiation program in a human hematopoietic cell line. UT-7. Exp. Hematol., 21, 665-670.
    • (1993) Exp. Hematol. , vol.21 , pp. 665-670
    • Nicolis, S.1    Ottolenghi, S.2    Papayannopoulos, T.3    Baiocchi, M.4    Migliaccio, G.5    Adamson, J.6    Migliaccio, A.R.7
  • 25
    • 0027394760 scopus 로고
    • Structures of sialylated oligosaccharides of human erythropoietin expressed in recombinant BHK-21 cells
    • Nimtz, M., Martin, W., Wray, V., Klöppel, K.D., Augustin, J. and Conradt, H.S. (1993) Structures of sialylated oligosaccharides of human erythropoietin expressed in recombinant BHK-21 cells. Eur. J. Biochem., 213, 39-56.
    • (1993) Eur. J. Biochem. , vol.213 , pp. 39-56
    • Nimtz, M.1    Martin, W.2    Wray, V.3    Klöppel, K.D.4    Augustin, J.5    Conradt, H.S.6
  • 26
    • 0028798520 scopus 로고
    • Immunogenicity of N-glycolylneuraminic acid-containing carbohydrate chains of recombinant human erythropoietin expressed in Chinese hamster ovary cells
    • Noguchi, A., Mukuria, C.J., Suzuki, E. and Naiki, M. (1995) Immunogenicity of N-glycolylneuraminic acid-containing carbohydrate chains of recombinant human erythropoietin expressed in Chinese hamster ovary cells. J. Biochem., 117, 59-62.
    • (1995) J. Biochem. , vol.117 , pp. 59-62
    • Noguchi, A.1    Mukuria, C.J.2    Suzuki, E.3    Naiki, M.4
  • 27
    • 0023792838 scopus 로고
    • Optimization of the tetrazolium-based colorimetric assay for the measurement of cell number and cytotoxicity
    • Page, M., Bejaoui, N., Cinq-Mars, B. and Lemieux, P. (1988) Optimization of the tetrazolium-based colorimetric assay for the measurement of cell number and cytotoxicity. Int. J. Immunopharmacol., 10, 785-793.
    • (1988) Int. J. Immunopharmacol. , vol.10 , pp. 785-793
    • Page, M.1    Bejaoui, N.2    Cinq-Mars, B.3    Lemieux, P.4
  • 28
    • 0030757724 scopus 로고    scopus 로고
    • Glycopeptide profiling of human urinary erythropoietin by matrix-assisted laser desorption/ionization mass spectrometry
    • Rahbek-Nielsen, H., Roepstorff, P., Reischl, H., Wozny, M., Koll, H. and Haselbeck, A. (1997) Glycopeptide profiling of human urinary erythropoietin by matrix-assisted laser desorption/ionization mass spectrometry. J. Mass Spectrom., 32, 948-958.
    • (1997) J. Mass Spectrom. , vol.32 , pp. 948-958
    • Rahbek-Nielsen, H.1    Roepstorff, P.2    Reischl, H.3    Wozny, M.4    Koll, H.5    Haselbeck, A.6
  • 29
    • 0026467185 scopus 로고
    • Quantitative mapping of the N-linked sialyl oligosaccharides of recombinant erythropoietin: Combination of direct high-performance anion-exchange chromatography and 2-aminopyridine derivatization
    • Rice, K.G., Takahashi, N., Namiki, Y., Tran, A.D., Lisi, P.J. and Lee, Y.C. (1992) Quantitative mapping of the N-linked sialyl oligosaccharides of recombinant erythropoietin: combination of direct high-performance anion-exchange chromatography and 2-aminopyridine derivatization. Anal. Biochem., 206, 278-287.
    • (1992) Anal. Biochem. , vol.206 , pp. 278-287
    • Rice, K.G.1    Takahashi, N.2    Namiki, Y.3    Tran, A.D.4    Lisi, P.J.5    Lee, Y.C.6
  • 30
    • 0027917747 scopus 로고
    • Peptide mapping and evaluation of glycopeptide microheterogeneity derived from endoproteinase digestion of erythropoietin by affinity high-performance capillary electrophoresis
    • Rush, R.S., Derby, P.L., Strickland, T.W. and Rohde, M.F. (1993) Peptide mapping and evaluation of glycopeptide microheterogeneity derived from endoproteinase digestion of erythropoietin by affinity high-performance capillary electrophoresis. Anal. Chem., 65, 1834-1842.
    • (1993) Anal. Chem. , vol.65 , pp. 1834-1842
    • Rush, R.S.1    Derby, P.L.2    Strickland, T.W.3    Rohde, M.F.4
  • 33
    • 0023645520 scopus 로고
    • Carbohydrate structure of erythropoietin expressed in Chinese hamster ovary cells by a human erythropoietin cDNA
    • Sasaki, H., Bothner, B., Dell, A. and Fukuda, M. (1987) Carbohydrate structure of erythropoietin expressed in Chinese hamster ovary cells by a human erythropoietin cDNA. J. Biol. Chem., 262, 12059-12076.
    • (1987) J. Biol. Chem. , vol.262 , pp. 12059-12076
    • Sasaki, H.1    Bothner, B.2    Dell, A.3    Fukuda, M.4
  • 34
    • 0023743771 scopus 로고
    • Site specific glycosylation of human recombinant erythropoietin: Analysis of glycopeptides or peptides at each glycosylation site by fast atom bombardment mass spectrometry
    • Sasaki, H., Ochi, N., Dell, A. and Fukuda, M. (1988) Site specific glycosylation of human recombinant erythropoietin: analysis of glycopeptides or peptides at each glycosylation site by fast atom bombardment mass spectrometry. Biochemistry, 27, 8618-8626.
    • (1988) Biochemistry , vol.27 , pp. 8618-8626
    • Sasaki, H.1    Ochi, N.2    Dell, A.3    Fukuda, M.4
  • 35
    • 0021014033 scopus 로고
    • Control of branching during the biosynthesis of asparagine-linked oligosaccharides
    • Schachter, H., Narasimhan, S., Gleeson, P. and Vella, G. (1983) Control of branching during the biosynthesis of asparagine-linked oligosaccharides. Can. J. Biochem. Cell Biol., 61, 1049-1066.
    • (1983) Can. J. Biochem. Cell Biol. , vol.61 , pp. 1049-1066
    • Schachter, H.1    Narasimhan, S.2    Gleeson, P.3    Vella, G.4
  • 36
    • 0024562079 scopus 로고
    • The in vitro metabolism of recombinant human erythropoietin in the rat
    • Spivak, J.L. and Hogans, B. (1989) The in vitro metabolism of recombinant human erythropoietin in the rat. Blood, 73, 90-99.
    • (1989) Blood , vol.73 , pp. 90-99
    • Spivak, J.L.1    Hogans, B.2
  • 37
    • 0025854778 scopus 로고
    • A subclass of cell surface carbohydrates revealed by a CHO mutant with two glycosylation mutations
    • Stanley, P., Sundaram, S. and Sallustio, S. (1991) A subclass of cell surface carbohydrates revealed by a CHO mutant with two glycosylation mutations. Glycobiology, 1, 307-314.
    • (1991) Glycobiology , vol.1 , pp. 307-314
    • Stanley, P.1    Sundaram, S.2    Sallustio, S.3
  • 38
    • 0025918707 scopus 로고
    • Structures and functional roles of the sugar chains of human erythropoietins
    • Takeuchi, M. and Kobata, A. (1991) Structures and functional roles of the sugar chains of human erythropoietins. Glycobiology, 1, 337-346.
    • (1991) Glycobiology , vol.1 , pp. 337-346
    • Takeuchi, M.1    Kobata, A.2
  • 39
    • 0023849601 scopus 로고
    • Comparative study of the asparagine-linked sugar chains of human erythropoietins purified from urine and the culture medium of recombinant Chinese Hamster Ovary cells
    • Takeuchi, M., Takasaki, S., Miyazaki, H., Kaato, T., Hoshit, S., Kochibe, N. and Kobata, A. (1988) Comparative study of the asparagine-linked sugar chains of human erythropoietins purified from urine and the culture medium of recombinant Chinese Hamster Ovary cells. J. Biol. Chem., 263, 3657-3663.
    • (1988) J. Biol. Chem. , vol.263 , pp. 3657-3663
    • Takeuchi, M.1    Takasaki, S.2    Miyazaki, H.3    Kaato, T.4    Hoshit, S.5    Kochibe, N.6    Kobata, A.7
  • 40
    • 0025277342 scopus 로고
    • Role of sugar chains in the in vitro biological activity of human erythropoietin produced in recombinant Chinese hamster ovary cells
    • Takeuchi, M., Takasaki, S., Shimada, M. and Kobata, A. (1990) Role of sugar chains in the in vitro biological activity of human erythropoietin produced in recombinant Chinese hamster ovary cells. J. Biol. Chem., 265, 12127-12130.
    • (1990) J. Biol. Chem. , vol.265 , pp. 12127-12130
    • Takeuchi, M.1    Takasaki, S.2    Shimada, M.3    Kobata, A.4
  • 42
    • 0025214337 scopus 로고
    • The role of carbohydrate in recombinant human erythropoietin
    • Tsuda, E., Kawanishi, G., Ueda, M., Masuda, S. and Sasaki, R. (1990) The role of carbohydrate in recombinant human erythropoietin. Eur. J. Biochem., 188, 405-411.
    • (1990) Eur. J. Biochem. , vol.188 , pp. 405-411
    • Tsuda, E.1    Kawanishi, G.2    Ueda, M.3    Masuda, S.4    Sasaki, R.5
  • 43
    • 0028267570 scopus 로고
    • Structure determination of the intact major sialylated oligosaccharide chains of recombinant human erythropoietin expressed in Chinese hamster ovary cells
    • Watson, E., Bhide, A. and van Halbeek, H. (1994) Structure determination of the intact major sialylated oligosaccharide chains of recombinant human erythropoietin expressed in Chinese hamster ovary cells. Glycobiology, 4, 227-237.
    • (1994) Glycobiology , vol.4 , pp. 227-237
    • Watson, E.1    Bhide, A.2    Van Halbeek, H.3
  • 44
    • 0024383145 scopus 로고
    • Recombinant human erythropoietin produced by Namalwa cells
    • Yanagi, H., Yoshima, T., Ogawa, I. and Okamoto, M. (1989) Recombinant human erythropoietin produced by Namalwa cells. DNA, 8, 419-427.
    • (1989) DNA , vol.8 , pp. 419-427
    • Yanagi, H.1    Yoshima, T.2    Ogawa, I.3    Okamoto, M.4
  • 45
    • 0033023018 scopus 로고    scopus 로고
    • Gas-liquid chromatography of the heptafluorobutyrate derivatives of the O-methyl-glycosides on capillary columns: A method for the quantitative determination of the monosaccharide composition of glycoproteins and glycolipids
    • Zanetta, J.-P., Timmerman, P. and Leroy, Y. (1999) Gas-liquid chromatography of the heptafluorobutyrate derivatives of the O-methyl-glycosides on capillary columns: a method for the quantitative determination of the monosaccharide composition of glycoproteins and glycolipids. Glycobiology, 9, 255-266.
    • (1999) Glycobiology , vol.9 , pp. 255-266
    • Zanetta, J.-P.1    Timmerman, P.2    Leroy, Y.3


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