Cathepsin L digestion of nanobioconjugates upon endocytosis

ACS Nano

Volumn 3, Issue 9, 2009, Pages 2461-2467

  Sée, Violaine ^a   Free, Paul ^b   Cesbron, Yann ^b   Nativo, Paula ^b,c   Shaheen, Umbreen ^b   Rigden, Daniel J ^b   Spiller, David G ^a   Fernig, David G ^b   White, Michael R H ^a   Prior, Ian A ^c   Brust, Mathias ^b   Lounis, Brahim ^d   Levy, Raphaël ^b  

^a Centre for Cell Imaging   (United Kingdom)

^b UNIVERSITY OF LIVERPOOL   (United Kingdom)

^c Physiological Laboratory   (United Kingdom)

^d UNIVERSITÉ DE BORDEAUX   (France)

Author keywords

Bionanotechnology; Endocytosis; Gold nanoparticles; Nanomedicine; Peptide; Protease; Self assembled monolayer

Indexed keywords

BIOMOLECULES; MAMMALS; MEDICAL NANOTECHNOLOGY; NANOPARTICLES; PEPTIDES; SELF ASSEMBLED MONOLAYERS;

BIOCONJUGATED NANOPARTICLES; BIOINFORMATICS TOOLS; BIOLOGICAL MOLECULE; BIONANOTECHNOLOGY; DEGRADATION PROCESS; ENDOCYTOSIS; ENDOSOMAL COMPARTMENTS; PROTEASE;

GOLD NANOPARTICLES;

CATHEPSIN L; GOLD; METAL NANOPARTICLE; PEPTIDE; PROTEOME;

AMINO ACID SEQUENCE; ARTICLE; BIOLOGY; CHEMISTRY; ENDOCYTOSIS; ENDOSOME; HELA CELL; HUMAN; METABOLISM; MOLECULAR GENETICS; PH;

AMINO ACID SEQUENCE; CATHEPSIN L; COMPUTATIONAL BIOLOGY; ENDOCYTOSIS; ENDOSOMES; GOLD; HELA CELLS; HUMANS; HYDROGEN-ION CONCENTRATION; METAL NANOPARTICLES; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEOME;

EID: 70349542908     PISSN: 19360851     EISSN: 1936086X     Source Type: Journal    
DOI: 10.1021/nn9006994     Document Type: Article

References (36)

1. Selhuber-Unkel, C.; Zins, I.; Schubert, O.; Sonnichsen, C.; Oddershede, L. B. Quantitative Optical Trapping of Single Gold Nanorods. Nano Lett. 2008, 8, 2998-3003.
2. Lasne, D.; Blab, G. A.; Berciaud, S.; Heine, M.; Groc, L.; Choquet, D.; Cognet, L.; Lounis, B. Single Nanoparticle Photothermal Tracking (SNaPT) of 5-nm Gold Beads in Live Cells. Biophys. J. 2006, 97, 4598-4604.
3. Courty, S.; Luccardini, C.; Bellaiche, Y.; Cappello, G.; Dahan, M. Tracking Individual Kinesin Motors in Living Cells Using Single Quantum-Dot Imaging. Nano Lett. 2006, 6, 1491-1495. (Pubitemid 44195334)
4. Boyer, D.; Tamarat, P.; Maali, A.; Lounis, B.; Orrit, M. Photothermal Imaging of Nanometer-Sized Metal Particles Among Scatterers. Science 2002, 297, 1160-1163.
5. Skala, M. C.; Crow, M. J.; Wax, A.; Izatt, J. A. Photothermal Optical Coherence Tomography of Epidermal Growth Factor Receptor in Live Cells Using Immunotargeted Gold Nanospheres. Nano Lett. 2008, 8, 3461-3467.
6. Lindfors, K.; Kalkbrenner, T.; Stoller, P.; Sandoghdar, V. Detection and Spectroscopy of Gold Nanoparticles Using Supercontinuum White Light Confocal Microscopy. Phys. Rev. Lett. 2004, 93, 037401.
7. Hell, S. W. Far-Field Optical Nanoscopy. Science 2007, 376, 1153-1158.
8. Sperling, R. A.; Gil, P. R.; Zhang, F.; Zanella, M.; Parak, W. J. Biological Applications of Gold Nanoparticles. Chem. Soc. Rev. 2008, 37, 1896-1908.
9. Khan, J. A.; Pillai, B.; Das, T. K.; Singh, Y.; Maiti, S. Molecular Effects of Uptake of Gold Nanoparticles in HeLa Cells. ChemBioChem 2007, 8, 1237-1240.
10. Pan, Y.; Neuss, S.; Leifert, A.; Fischler, M.; Wen, F.; Simon, U.; Schmid, G.; Brandau, W.; Jahnen-Dechent, W. Size- Dependent Cytotoxicity of Gold Nanoparticles. Small 2007, 3, 1941-1949.
11. Tsoli, M.; Kuhn, H.; Brandau, W.; Esche, H.; Schmid, G. Cellular Uptake and Toxicity of Au(55) Clusters. Small 2005, 7, 841-844.
12. Liu, Y. L.; Shipton, M. K.; Ryan, J.; Kaufman, E. D.; Franzen, S.; Feldheim, D. L. Synthesis, Stability, and Cellular Internalization of Gold Nanoparticles Containing Mixed Peptidepoly(ethylene glycol) Monolayers. Anal. Chem. 2007, 79, 2221-2229.
13. Nativo, P.; Prior, I. A.; Brust, M. Uptake and Intracellular Fate of Surface-Modified Gold Nanoparticles. ACS Nano 2008, 2, 1639-1644.
14. Verma, A.; Uzun, O.; Hu, Y. H.; Hu, Y.; Han, H. S.; Watson, N.; Chen, S. L.; Irvine, D. J.; Stellacci, F. Surface-Structure- Regulated Cell-Membrane Penetration by Monolayer- Protected Nanoparticles. Nat. Mater. 2008, 7, 588-595.
15. Chithrani, B. D.; Chan, W. C. W. Elucidating the Mechanism of Cellular Uptake and Removal of Protein-Coated Gold Nanoparticles of Different Sizes and Shapes. Nano Lett. 2007, 7, 1542-1550.
16. Ryan, J. A.; Overton, K. W.; Speight, M. E.; Oldenburg, C. M.; Loo, L.; Robarge, W.; Franzen, S.; Feldheim, D. L. Cellular Uptake of Gold Nanoparticles Passivated with BSA-SV40 Large T Antigen Conjugates. Anal. Chem. 2007, 79, 9150-9159.
17. Hong, R.; Han, G.; Fernandez, J. M.; Kim, B. J.; Forbes, N. S.; Rotello, V. M. Glutathione-Mediated Delivery and Release Using Monolayer Protected Nanoparticle Carriers. J. Am. Chem. Soc. 2006, 728, 1078-1079.
18. Han, G.; Chari, N. S.; Verma, A.; Hong, R.; Martin, C. T.; Rotello, V. M. Controlled Recovery of the Transcription of Nanoparticle-Bound DNA by Intracellular Concentrations of Glutathione. Bioconjugate Chem. 2005, 76, 1356-1359.
19. Levy, R. Peptide-Capped Gold Nanoparticles: Towards Artificial Proteins. ChemBioChem 2006, 7, 1141-1145.
20. Levy, R.; Thanh, N. T. K.; Doty, R. C.; Hussain, I.; Nichols, R. J.; Schiffrin, D. J.; Brust, M.; Fernig, D. G. Rational and Combinatorial Design of Peptide Capping Ligands for Gold Nanoparticles. J. Am. Chem. Soc. 20 04, 726, 10076-10084.
21. Doty, R. C.; Tshikhudo, T. R.; Brust, M.; Fernig, D. G. Extremely Stable Water-Soluble Ag Nanoparticles. Chem. Mater. 2005, 77, 4630-4635.
22. Michalet, X.; Pinaud, F. F.; Bentolila, L. A.; Tsay, J. M.; Doose, S.; Li, J. J.; Sundaresan, G.; Wu, A. M.; Gambhir, S. S.; Weiss, S. Quantum Dots for Live Cells, In Vivo Imaging, and Diagnostics. Science 2005, 307, 538-544.
23. Sarikaya, M.; Tamerler, C.; Jen, A. K. Y.; Schulten, K.; Baneyx, F. Molecular Biomimetics: Nanotechnology through Biology. Nat. Mater. 2003, 2, 577-585.
24. Kuhn, S.; Hakanson, U.; Rogobete, L.; Sandoghdar, V. Enhancement of Single Molecule Fluorescence Using a Gold Nanoparticle as an Optical Nanoantenna. Phys. Rev. Lett. 2006, 97, 017402.
25. Dulkeith, E.; Ringler, M.; Klar, T. A.; Feldmann, J.; Javier, A. M.; Parak, W. J. Gold Nanoparticles Quench Fluorescence by Phase Induced Radiative Rate Suppression. Nano Lett. 2005, 5, 585-589.
26. Lee, S.; Cha, E. J.; Park, K.; Lee, S. Y.; Hong, J. K.; Sun, I. C.; Kim, S. Y.; Choi, K.; Kwon, I. C.; Kim, K.; Ahn, C. H. A Near- Infrared-Fluorescence- Quenched Gold-Nanoparticle Imaging Probe for In Vivo Drug Screening and Protease Activity Determination. Angew. Chem., Int. Ed. 2008, 47, 2804-2807.
27. Seglen, P. O.; Grinde, B.; Solheim, A. E. Inhibition of the Lysosomal Pathway of Protein-Degradation in Isolated Rat Hepatocytes by Ammonia, Methylamine, Chloroquine and Leupeptin. Eur. J. Biochem. 1979, 95, 215-225.
28. Wadia, J. S.; Stan, R. V.; Dowdy, S. F. Transducible TAT-HA Fusogenic Peptide Enhances Escape ofTAT-Fusion Proteins after Lipid Raft Macropinocytosis. Nat. Med. 2004, 70, 310-315.
29. Sandhu, K. K.; McIntosh, C. M.; Simard, J. M.; Smith, S. W.; Rotello, V. M. Gold Nanoparticle-Mediated Transfection of Mammalian Cells. Bioconjugate Chem. 2002, 73, 3-6.
30. Seleverstov, O.; Phang, J. M.; Zabirnyk, O. Semiconductor Nanocrystals in Autophagy Research: Methodology Improvement at Nanosized Scale. Methods Enzymol. 2009, 452, 277-296.
31. Pillay, C. S.; Elliott, E.; Dennison, C. Endolysosomal Proteolysis and Its Regulation. Biochem. J. 2002, 363, 417-429.
32. Kircher, M. F.; Weissleder, R.; Josephson, L. A Dual Fluorochrome Probe for Imaging Proteases. Bioconjugate Chem. 2004, 75, 242-248.
33. Berciaud, S.; Cognet, L.; Blab, G. A.; Lounis, B. Photothermal Heterodyne Imaging of Individual Non-fluorescent Nanoclusters and Nanocrystals. Phys. Rev. Lett. 2004, 93, 257402.
34. Puzer, L.; Cotrin, S. S.; Alves, M. F. M.; Egborge, T.; Araujo, M. S.; Juliano, M. A.; Juliano, L.; Bromme, D.; Carmona, A. K. Comparative Substrate Specificity Analysis of Recombinant Human Cathepsin V and Cathepsin L. Arch. Biochem. Biophys. 2004, 430, 274-283.
35. Portaro, F. C. V.; Santos, A. B. F.; Cezari, M. H. S.; Juliano, M. A.; Juliano, L.; Carmona, E. Probing the Specificity of Cysteine Proteinases at Subsites Remote from the Active Site: Analysis of P-4, P-3, P-2= and P-3= Variations in Extended Substrates. Biochem. J. 2000, 347, 123-129.
36. Menard, R.; Carmona, E.; Plouffe, C.; Bromme, D.; Konishi, Y.; Lefebvre, J.; Storer, A. C.The Specificity of the S(1)= Subsite of Cysteine Proteases. FEBS Lett. 1993, 328, 107-110.